[논문]Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes

Petela, Naomi J; Gonzalez Llamazares, Andres; Dixon, Sarah; Hu, Bin; Lee, Byung-Gil; Metson, Jean; Seo, Heekyo; Ferrer-Harding, Antonio; Voulgaris, Menelaos; Gligoris, Thomas; Collier, James; Oh, Byung-Ha; Lö; we, Jan; Nasmyth, Kim A

doi:10.7554/elife.67268

[해외논문] Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes 원문보기

eLife, v.10, 2021년, pp.e67268 -

Petela, Naomi J (Department of Biochemistry, University of Oxford Oxford United Kingdom) , Gonzalez Llamazares, Andres (MRC Laboratory of Molecular Biology Cambridge United Kingdom) , Dixon, Sarah (Department of Biochemistry, University of Oxford Oxford United Kingdom) , Hu, Bin (Institute of Medical Sciences, University of Aberdeen Aberdeen United Kingdom) , Lee, Byung-Gil (MRC Laboratory of Molecular Biology Cambridge United Kingdom) , Metson, Jean (Department of Biochemistry, University of Oxford Oxford United Kingdom) , Seo, Heekyo (Department of Biological Sciences, KAIST Institute for the Biocentury, Cancer Metastasis Control Center, Korea Advanced Institute of Science and Technology Daejeon Republic of Korea) , Ferrer-Harding, Antonio (Department of Biochemistry, University of Oxford Oxford United Kingdom) , Voulgaris, Menelaos (Department of Biochemistry, Univers) , Gligoris, Thomas , Collier, James , Oh, Byung-Ha , Löwe, Jan , Nasmyth, Kim A

Abstract ▼ AI-Helper

Cohesin’s association with and translocation along chromosomal DNAs depend on an ATP hydrolysis cycle driving the association and subsequent release of DNA. This involves DNA being ‘clamped’ by Scc2 and ATP-dependent engagement of cohesin’s Smc1 and Smc3 head domains. Scc2’s replacement by Pds5 abrogates cohesin’s ATPase and has an important role in halting DNA loop extrusion. The ATPase domains of all SMC proteins are separated from their hinge dimerisation domains by 50-nm-long coiled coils, which have been observed to zip up along their entire length and fold around an elbow, thereby greatly shortening the distance between hinges and ATPase heads. Whether folding exists in vivo or has any physiological importance is not known. We present here a cryo-EM structure of the apo form of cohesin that reveals the structure of folded and zipped-up coils in unprecedented detail and shows that Scc2 can associate with Smc1’s ATPase head even when it is fully disengaged from that of Smc3. Using cysteine-specific crosslinking, we show that cohesin’s coiled coils are frequently folded in vivo, including when cohesin holds sister chromatids together. Moreover, we describe a mutation (SMC1D588Y) within Smc1’s hinge that alters how Scc2 and Pds5 interact with Smc1’s hinge and that enables Scc2 to support loading in the absence of its normal partner Scc4. The mutant phenotype of loading without Scc4 is only explicable if loading depends on an association between Scc2/4 and cohesin’s hinge, which in turn requires coiled coil folding.

참고문헌 (68)

Beckouët F Srinivasan M Roig MB Chan KL Scheinost JC Batty P Hu B Petela N Gligoris T Smith AC Strmecki L Rowland BD Nasmyth K 2016 Releasing activity disengages cohesin's Smc3/Scc1 Interface in a Process Blocked by Acetylation Molecular Cell 61 563 574 10.1016/j.molcel.2016.01.026 26895425

상세보기
Bürmann F Lee BG Than T Sinn L O'Reilly FJ Yatskevich S Rappsilber J Hu B Nasmyth K Löwe J 2019 A folded conformation of MukBEF and cohesin Nature Structural & Molecular Biology 26 227 236 10.1038/s41594-019-0196-z 30833788

상세보기
Chan KL Roig MB Hu B Beckouët F Metson J Nasmyth K 2012 Cohesin's DNA exit gate is distinct from its entrance gate and is regulated by acetylation Cell 150 961 974 10.1016/j.cell.2012.07.028 22901742

상세보기
Chan KL Gligoris T Upcher W Kato Y Shirahige K Nasmyth K Beckouët F 2013 Pds5 promotes and protects cohesin acetylation PNAS 110 13020 13025 10.1073/pnas.1306900110 23878248

상세보기
Chao WC Murayama Y Muñoz S Costa A Uhlmann F Singleton MR 2015 Structural studies reveal the functional modularity of the Scc2-Scc4 cohesin loader Cell Reports 12 719 725 10.1016/j.celrep.2015.06.071 26212329

상세보기
Chapard C Jones R van Oepen T Scheinost JC Nasmyth K 2019 Sister DNA entrapment between juxtaposed smc heads and kleisin of the cohesin complex Molecular Cell 75 224 237 10.1016/j.molcel.2019.05.023 31201089

상세보기
Ciosk R Shirayama M Shevchenko A Tanaka T Toth A Shevchenko A Nasmyth K 2000 Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins Molecular Cell 5 243 254 10.1016/S1097-2765(00)80420-7 10882066

상세보기
Collier JE Lee BG Roig MB Yatskevich S Petela NJ Metson J Voulgaris M Gonzalez Llamazares A Löwe J Nasmyth KA 2020 Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3 eLife 9 e59560 10.7554/eLife.59560 32930661

상세보기
Dauban L Montagne R Thierry A Lazar-Stefanita L Bastié N Gadal O Cournac A Koszul R Beckouët F 2020 Regulation of Cohesin-Mediated chromosome folding by Eco1 and other partners Molecular Cell 77 1279 1293 10.1016/j.molcel.2020.01.019 32032532

상세보기
Davidson IF Bauer B Goetz D Tang W Wutz G Peters JM 2019 DNA loop extrusion by human cohesin Science 366 1338 1345 10.1126/science.aaz3418 31753851

상세보기
Diebold-Durand ML Lee H Ruiz Avila LB Noh H Shin HC Im H Bock FP Bürmann F Durand A Basfeld A Ham S Basquin J Oh BH Gruber S 2017 Structure of Full-Length SMC and rearrangements required for chromosome organization Molecular Cell 67 334 347 10.1016/j.molcel.2017.06.010 28689660

상세보기
Dujon B Sherman D Fischer G Durrens P Casaregola S Lafontaine I De Montigny J Marck C Neuvéglise C Talla E Goffard N Frangeul L Aigle M Anthouard V Babour A Barbe V Barnay S Blanchin S Beckerich JM Beyne E Bleykasten C Boisramé A Boyer J Cattolico L Confanioleri F De Daruvar A Despons L Fabre E Fairhead C Ferry-Dumazet H Groppi A Hantraye F Hennequin C Jauniaux N Joyet P Kachouri R Kerrest A Koszul R Lemaire M Lesur I Ma L Muller H Nicaud JM Nikolski M Oztas S Ozier-Kalogeropoulos O Pellenz S Potier S Richard GF Straub ML Suleau A Swennen D Tekaia F Wésolowski-Louvel M Westhof E Wirth B Zeniou-Meyer M Zivanovic<

상세보기
Elbatsh AMO Haarhuis JHI Petela N Chapard C Fish A Celie PH Stadnik M Ristic D Wyman C Medema RH Nasmyth K Rowland BD 2016 Cohesin releases DNA through asymmetric ATPase-Driven ring opening Molecular Cell 61 575 588 10.1016/j.molcel.2016.01.025 26895426

상세보기
Emsley P Lohkamp B Scott WG Cowtan K 2010 Features and development of coot Acta Crystallographica. Section D, Biological Crystallography 66 10.1107/S0907444910007493 20383002

상세보기
Ganji M Shaltiel IA Bisht S Kim E Kalichava A Haering CH Dekker C 2018 Real-time imaging of DNA loop extrusion by condensin Science 360 102 105 10.1126/science.aar7831 29472443

상세보기
Gligoris TG Scheinost JC Bürmann F Petela N Chan KL Uluocak P Beckouët F Gruber S Nasmyth K Löwe J 2014 Closing the cohesin ring: structure and function of its Smc3-kleisin interface Science 346 963 967 10.1126/science.1256917 25414305

상세보기
Goddard TD Huang CC Meng EC Pettersen EF Couch GS Morris JH Ferrin TE 2018 UCSF ChimeraX: meeting modern challenges in visualization and analysis Protein Science 27 14 25 10.1002/pro.3235 28710774

상세보기
Golfier S Quail T Kimura H Brugués J 2020 Cohesin and condensin extrude DNA loops in a cell cycle-dependent manner eLife 9 e53885 10.7554/eLife.53885 32396063

상세보기
Gruber S Arumugam P Katou Y Kuglitsch D Helmhart W Shirahige K Nasmyth K 2006 Evidence that loading of cohesin onto chromosomes involves opening of its SMC hinge Cell 127 523 537 10.1016/j.cell.2006.08.048 17081975

상세보기
Guacci V Stricklin J Bloom MS Gu X Bhatter M Koshland D 2015 A novel mechanism for the establishment of sister chromatid cohesion by the ECO1 acetyltransferase Molecular Biology of the Cell 26 117 133 10.1091/mbc.e14-08-1268 25378582

상세보기
Haering CH Löwe J Hochwagen A Nasmyth K 2002 Molecular architecture of SMC proteins and the yeast cohesin complex Molecular Cell 9 773 788 10.1016/S1097-2765(02)00515-4 11983169

상세보기
Haering CH Schoffnegger D Nishino T Helmhart W Nasmyth K Löwe J 2004 Structure and stability of cohesin's Smc1-kleisin interaction Molecular Cell 15 951 964 10.1016/j.molcel.2004.08.030 15383284

상세보기
Haering CH Farcas AM Arumugam P Metson J Nasmyth K 2008 The cohesin ring concatenates sister DNA molecules Nature 454 297 301 10.1038/nature07098 18596691

상세보기
Hassler M Shaltiel IA Haering CH 2018 Towards a unified model of SMC complex function Current Biology 28 R1266 R1281 10.1016/j.cub.2018.08.034 30399354

상세보기
Higashi TL Eickhoff P Sousa JS Locke J Nans A Flynn HR Snijders AP Papageorgiou G O'Reilly N Chen ZA O'Reilly FJ Rappsilber J Costa A Uhlmann F 2020 A Structure-Based mechanism for DNA entry into the cohesin ring Molecular Cell 79 917 933 10.1016/j.molcel.2020.07.013 32755595

상세보기
Hinshaw SM Makrantoni V Kerr A Marston AL Harrison SC 2015 Structural evidence for Scc4-dependent localization of cohesin loading eLife 4 e06057 10.7554/eLife.06057 26038942

상세보기
Hinshaw SM Makrantoni V Harrison SC Marston AL 2017 The kinetochore receptor for the cohesin loading complex Cell 171 72 84 10.1016/j.cell.2017.08.017 28938124

상세보기
Hu B Itoh T Mishra A Katoh Y Chan KL Upcher W Godlee C Roig MB Shirahige K Nasmyth K 2011 ATP hydrolysis is required for relocating cohesin from sites occupied by its Scc2/4 loading complex Current Biology 21 12 24 10.1016/j.cub.2010.12.004 21185190

상세보기
Hu B Petela N Kurze A Chan K-L Chapard C Nasmyth K 2015 Biological chromodynamics: a general method for measuring protein occupancy across the genome by calibrating ChIP-seq Nucleic Acids Research 21 gkv670 10.1093/nar/gkv670

상세보기
Hu B 2018 ATP hydrolysis is required for relocating cohesin from sites occupied by its Scc2/4 loading complex Current Biology : CB
Huang J Hsu JM Laurent BC 2004 The RSC nucleosome-remodeling complex is required for cohesin's association with chromosome arms Molecular Cell 13 739 750 10.1016/S1097-2765(04)00103-0 15023343

상세보기
Huis in 't Veld PJ Herzog F Ladurner R Davidson IF Piric S Kreidl E Bhaskara V Aebersold R Peters JM 2014 Characterization of a DNA exit gate in the human cohesin ring Science 346 968 972 10.1126/science.1256904 25414306

상세보기
Kikuchi S Borek DM Otwinowski Z Tomchick DR Yu H 2016 Crystal structure of the cohesin loader Scc2 and insight into cohesinopathy PNAS 113 12444 12449 10.1073/pnas.1611333113 27791135

상세보기
Kim Y Shi Z Zhang H Finkelstein IJ Yu H 2019 Human cohesin compacts DNA by loop extrusion Science 366 1345 1349 10.1126/science.aaz4475 31780627

상세보기
Kueng S Hegemann B Peters BH Lipp JJ Schleiffer A Mechtler K Peters JM 2006 Wapl controls the dynamic association of cohesin with chromatin Cell 127 955 967 10.1016/j.cell.2006.09.040 17113138

상세보기
Kurze A Michie KA Dixon SE Mishra A Itoh T Khalid S Strmecki L Shirahige K Haering CH Löwe J Nasmyth K 2011 A positively charged channel within the Smc1/Smc3 hinge required for sister chromatid cohesion The EMBO Journal 30 364 378 10.1038/emboj.2010.315 21139566

상세보기
Langmead B Salzberg SL 2012 Fast gapped-read alignment with bowtie 2 Nature Methods 9 357 359 10.1038/nmeth.1923 22388286

상세보기
Lee BG Roig MB Jansma M Petela N Metson J Nasmyth K Löwe J 2016 Crystal structure of the cohesin gatekeeper Pds5 and in complex with kleisin Scc1 Cell Reports 14 2108 2115 10.1016/j.celrep.2016.02.020 26923598

상세보기
Lee BG Merkel F Allegretti M Hassler M Cawood C Lecomte L O'Reilly FJ Sinn LR Gutierrez-Escribano P Kschonsak M Bravo S Nakane T Rappsilber J Aragon L Beck M Löwe J Haering CH 2020 Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism Nature Structural & Molecular Biology 27 743 751 10.1038/s41594-020-0457-x 32661420

상세보기
Lopez-Serra L Kelly G Patel H Stewart A Uhlmann F 2014 The Scc2-Scc4 complex acts in sister chromatid cohesion and transcriptional regulation by maintaining nucleosome-free regions Nature Genetics 46 1147 1151 10.1038/ng.3080 25173104

상세보기
Mishra A Hu B Kurze A Beckouët F Farcas AM Dixon SE Katou Y Khalid S Shirahige K Nasmyth K 2010 Both interaction surfaces within cohesin's hinge domain are essential for its stable chromosomal association Current Biology 20 279 289 10.1016/j.cub.2009.12.059 20153193

상세보기
Muir KW Li Y Weis F Panne D 2020 The structure of the cohesin ATPase elucidates the mechanism of SMC-kleisin ring opening Nature Structural & Molecular Biology 27 233 239 10.1038/s41594-020-0379-7 32066964

상세보기
Muñoz S Minamino M Casas-Delucchi CS Patel H Uhlmann F 2019 A role for chromatin remodeling in cohesin loading onto chromosomes Molecular Cell 74 664 673 10.1016/j.molcel.2019.02.027 30922844

상세보기
Murayama Y Uhlmann F 2015 DNA entry into and exit out of the cohesin ring by an interlocking gate mechanism Cell 163 1628 1640 10.1016/j.cell.2015.11.030 26687354

상세보기
Nasmyth KA 1982 Molecular genetics of yeast mating type Annual Review of Genetics 16 439 500 10.1146/annurev.ge.16.120182.002255 6760802

상세보기
Nicol JW Helt GA Blanchard SG Raja A Loraine AE 2009 The integrated genome browser: free software for distribution and exploration of genome-scale datasets Bioinformatics 25 2730 2731 10.1093/bioinformatics/btp472 19654113

상세보기
Paldi F Alver B Robertson D Schalbetter SA Kerr A Kelly DA Baxter J Neale MJ Marston AL 2020 Convergent genes shape budding yeast pericentromeres Nature 582 119 123 10.1038/s41586-020-2244-6 32494069

상세보기
Petela NJ Gligoris TG Metson J Lee BG Voulgaris M Hu B Kikuchi S Chapard C Chen W Rajendra E Srinivisan M Yu H Löwe J Nasmyth KA 2018 Scc2 is a potent activator of cohesin's ATPase that Promotes Loading by Binding Scc1 without Pds5 Molecular Cell 70 1134 1148 10.1016/j.molcel.2018.05.022 29932904

상세보기
Punjani A Rubinstein JL Fleet DJ Brubaker MA 2017 cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination Nature Methods 14 290 296 10.1038/nmeth.4169 28165473

상세보기
Rolef Ben-Shahar T Heeger S Lehane C East P Flynn H Skehel M Uhlmann F 2008 Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion Science 321 563 566 10.1126/science.1157774 18653893

상세보기
Rosenthal PB Henderson R 2003 Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy Journal of Molecular Biology 333 721 745 10.1016/j.jmb.2003.07.013 14568533

상세보기
Shi Z Gao H Bai XC Yu H 2020 Cryo-EM structure of the human cohesin-NIPBL-DNA complex Science 368 1454 1459 10.1126/science.abb0981 32409525

상세보기
Sievers F Wilm A Dineen D Gibson TJ Karplus K Li W Lopez R McWilliam H Remmert M Söding J Thompson JD Higgins DG 2011 Fast, scalable generation of high-quality protein multiple sequence alignments using clustal omega Molecular Systems Biology 7 539 10.1038/msb.2011.75 21988835

상세보기
Soh YM Bürmann F Shin HC Oda T Jin KS Toseland CP Kim C Lee H Kim SJ Kong MS Durand-Diebold ML Kim YG Kim HM Lee NK Sato M Oh BH Gruber S 2015 Molecular basis for SMC rod formation and its dissolution upon DNA binding Molecular Cell 57 290 303 10.1016/j.molcel.2014.11.023 25557547

상세보기
Srinivasan M Scheinost J Petela N Gligoris T Wissler M Ogushi S Collier J Voulgaris M Kurze A Chan K-L Hu B Costanzo V Nasmyth K 2018 The cohesin ring uses its hinge to organize DNA using non-topological as well as topological mechanisms bioRxiv 10.1101/197848
Srinivasan M Petela NJ Scheinost JC Collier J Voulgaris M B Roig M Beckouët F Hu B Nasmyth KA 2019 Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1 eLife 8 e44736 10.7554/eLife.44736 31225797

상세보기
Tóth A Ciosk R Uhlmann F Galova M Schleiffer A Nasmyth K 1999 Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication Genes & Development 13 320 333 10.1101/gad.13.3.320 9990856

상세보기
Turk D 2013 MAIN software for density averaging, model building, structure refinement and validation Acta Crystallographica Section D Biological Crystallography 69 1342 1357 10.1107/S0907444913008408 23897458

상세보기
Uhlmann F 2001 Chromosome cohesion and segregation in mitosis and meiosis Current Opinion in Cell Biology 13 754 761 10.1016/S0955-0674(00)00279-9 11698193

상세보기
Unal E Heidinger-Pauli JM Kim W Guacci V Onn I Gygi SP Koshland DE 2008 A molecular determinant for the establishment of sister chromatid cohesion Science 321 566 569 10.1126/science.1157880 18653894

상세보기
Wagner T Merino F Stabrin M Moriya T Antoni C Apelbaum A Hagel P Sitsel O Raisch T Prumbaum D Quentin D Roderer D Tacke S Siebolds B Schubert E Shaikh TR Lill P Gatsogiannis C Raunser S 2019 SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM Communications Biology 2 218 10.1038/s42003-019-0437-z 31240256

상세보기
Waterhouse A Bertoni M Bienert S Studer G Tauriello G Gumienny R Heer FT de Beer TAP Rempfer C Bordoli L Lepore R Schwede T 2018 SWISS-MODEL: homology modelling of protein structures and complexes Nucleic Acids Research 46 W296 W303 10.1093/nar/gky427 29788355

상세보기
White CL Suto RK Luger K 2001 Structure of the yeast nucleosome core particle reveals fundamental changes in internucleosome interactions The EMBO Journal 20 5207 5218 10.1093/emboj/20.18.5207 11566884

상세보기
Wilkinson ME Kumar A Casañal A 2019 Methods for merging data sets in electron cryo-microscopy Acta Crystallographica Section D Structural Biology 75 782 791 10.1107/S2059798319010519 31478901

상세보기
Wutz G Várnai C Nagasaka K Cisneros DA Stocsits RR Tang W Schoenfelder S Jessberger G Muhar M Hossain MJ Walther N Koch B Kueblbeck M Ellenberg J Zuber J Fraser P Peters JM 2017 Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins The EMBO Journal 36 3573 3599 10.15252/embj.201798004 29217591

상세보기
Zhang K 2016 Gctf: real-time CTF determination and correction Journal of Structural Biology 193 1 12 10.1016/j.jsb.2015.11.003 26592709

상세보기
Zheng SQ Palovcak E Armache JP Verba KA Cheng Y Agard DA 2017 MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy Nature Methods 14 331 332 10.1038/nmeth.4193 28250466

상세보기
Zivanov J Nakane T Forsberg BO Kimanius D Hagen WJ Lindahl E Scheres SH 2018 New tools for automated high-resolution cryo-EM structure determination in RELION-3 eLife 7 e42166 10.7554/eLife.42166 30412051

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