[논문]Clinical Trial: Magnetoplasmonic ELISA for Urine-based Active Tuberculosis Detection and Anti-Tuberculosis Therapy Monitoring

Kim, Jeonghyo; Tran, Van Tan; Oh, Sangjin; Jang, Minji; Lee, Dong Kun; Hong, Jong Chul; Park, Tae Jung; Kim, Hwa-Jung; Lee, Jaebeom

doi:10.1021/acscentsci.1c00948

[해외논문] Clinical Trial: Magnetoplasmonic ELISA for Urine-based Active Tuberculosis Detection and Anti-Tuberculosis Therapy Monitoring 원문보기

ACS central science, v.7 no.11, 2021년, pp.1898 - 1907

Kim, Jeonghyo (Department of Chemistry , Chungnam National University , Daejeon 34134 , Republic of Korea) , Tran, Van Tan (Department of Chemistry , Chungnam National University , Daejeon 34134 , Republic of Korea) , Oh, Sangjin (Department of Chemistry , Chungnam National University , Daejeon 34134 , Republic of Korea) , Jang, Minji (Department of Cogno-Mechatronics Engineering , Pusan National University , Busan 46241 , Republic of Korea) , Lee, Dong Kun (Department of Otolaryngology, Head and Neck Surgery, College of Medicine , Dong-A University , Busan 49201 , Republic of Korea) , Hong, Jong Chul (Hana Otorhinolaryngology Hospital , Ulsan 44694 , Republic of Korea) , Park, Tae Jung (Department of Chemistry , Chung-Ang University , Seoul 06974 , Republic of Korea) , Kim, Hwa-Jung , Lee, Jaebeom

Abstract ▼ AI-Helper

The coronavirus disease 2019 (COVID-19) pandemic has proved the importance of fast and widespread diagnostic testing to prevent serious epidemics timely. The first-line weapon against rapidly transmitted disease is a quick and massive screening test to isolate patients immediately, preventing dissemination. Here, we described magnetoplasmonic nanozymes (MagPlas NZs), i.e., hierarchically coassembled Fe3O4-Au superparticles, that are capable of integrating magnetic enrichment and catalytic amplification, thereby the assay can be streamlined amenable to high-throughput operation and achieve ultrahigh sensitivity. Combining this advantage with conventional enzyme-linked immunosorbent assay (ELISA), we propose a MagPlas ELISA for urine-based tuberculosis (TB) diagnosis and anti-TB therapy monitoring, which enables fast (<3 h), and highly sensitive (up to pM with naked-eyes, < 10 fM with plate reader) urinary TB antigen detection. A clinical study with a total of 297 urine samples showed robust sensitivity for pulmonary tuberculosis (85.0%) and extra-pulmonary tuberculosis (52.8%) patients with high specificity (96.7% and 96.9%). Furthermore, this methodology offers a great promise of noninvasive therapeutic response monitoring, which is impracticable in the gold-standard culture method. The MagPlas ELISA showed high sensitivity comparable to the PCR assay while retaining a simple and cheap ELISA concept, thus it could be a promising point-of-care test for TB epidemic control and possibly applied to other acute infections.Multifunctional Fe3O4−Au nanozyme-integrated ELISA enables fast and highly sensitive urinary tuberculosis biomarker detection and noninvasive therapeutic response monitoring in patients’ urine.[FIG OMISSION]

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