[논문]Longitudinal Intravital Imaging of Tumor-Infiltrating Lymphocyte Motility in Breast Cancer Models

Park, Inwon; Hong, Sujung; Seok, Joon; Lucia, Stephani Edwina; Song, Eunjoo; Kim, Mingyo; Kong, Eunji; Seo, Howon; Hwang, Yoonha; Ahn, Soyeon; Kim, Seonghye; Jang, Dong-Hyun; Lee, Jae Hyuk; Park, Su-Hyung; Kim, Pilhan; Jo, You Hwan

doi:10.4048/jbc.2021.24.e40

[국내논문] Longitudinal Intravital Imaging of Tumor-Infiltrating Lymphocyte Motility in Breast Cancer Models 원문보기

Journal of breast cancer= 한국유방암학회지, v.24, 2021년, pp.463 - 473

Park, Inwon (Department of Emergency Medicine, Seoul National University Bundang Hospital (SNUBH), Seongnam, Korea .) , Hong, Sujung (Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea .) , Seok, Joon (Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea .) , Lucia, Stephani Edwina (KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea .) , Song, Eunjoo (Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea .) , Kim, Mingyo (Division of Rheumatology, Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju, Korea .) , Kong, Eunji (KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST) , Seo, Howon , Hwang, Yoonha , Ahn, Soyeon , Kim, Seonghye , Jang, Dong-Hyun , Lee, Jae Hyuk , Park, Su-Hyung , Kim, Pilhan , Jo, You Hwan

Abstract ▼ AI-Helper

Immunoreactive dynamics of tumor-infiltrating lymphocytes (TILs) within the tumor microenvironment in breast cancer are not well understood. This study aimed to investigate the spatiotemporal cellular dynamics of TILs in breast cancer models. Breast cancer cells were implanted into the dorsal skinfold chamber of BALB/c nude mice, and T lymphocytes were adoptively transferred. Longitudinal intravital imaging was performed, and the spatiotemporal dynamics of TILs were assessed. In the 4T1 model, TILs progressively exhibited increased motility, and their motility inside the tumor was significantly higher than that outside the tumor. In the MDA-MB-231 model, the motility of TILs progressively decreased after an initial increase. TIL motility in the MDA-MB-231 and MCF-7 models differed significantly, suggesting an association between programmed death-ligand 1 expression levels and TIL motility, which warrants further investigation. Furthermore, intravital imaging of TILs can be a useful method for addressing dynamic interactions between TILs and breast cancer cells.

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DOI : 10.4048/jbc.2021.24.e40 [무료]
한국유방암학회 : 저널
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