[논문]L-Cysteine Increases the Transformation Efficiency of Chinese Cabbage ( Brassica rapa ssp. pekinensis )

Sivanandhan, Ganeshan; Moon, Jiae; Sung, Chaemin; Bae, Solhee; Yang, Zhi Hong; Jeong, So Young; Choi, Su Ryun; Kim, Sang-Gyu; Lim, Yong Pyo

doi:10.3389/fpls.2021.767140

[해외논문] L-Cysteine Increases the Transformation Efficiency of Chinese Cabbage ( Brassica rapa ssp. pekinensis ) 원문보기

Frontiers in plant science, v.12, 2021년, pp.767140 -

Sivanandhan, Ganeshan (Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Science, Chungnam National University , Daejeon , South Korea) , Moon, Jiae (Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Science, Chungnam National University , Daejeon , South Korea) , Sung, Chaemin (Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Science, Chungnam National University , Daejeon , South Korea) , Bae, Solhee (Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Science, Chungnam National University , Daejeon , South Korea) , Yang, Zhi Hong (Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Science, Chungnam National University , Daejeon , South Korea) , Jeong, So Young , Choi, Su Ryun , Kim, Sang-Gyu , Lim, Yong Pyo

Abstract ▼ AI-Helper

Successful Agrobacterium-mediated transformations of Chinese cabbage have been limited owing to the plant’s recalcitrant nature, genomic background and explant necrosis upon infection, which hinders the transfer of T-DNA region into the Chinese cabbage. Consequently, in the current experiment, a stable Agrobacterium tumefaciens-mediated transformation method for Chinese cabbage cv. Kenshin established by employing important anti-oxidants in the co-cultivation and subsequent regeneration media. Four-day-old in vitro derived cotyledon explants were infected with A. tumefaciens strain GV3101 harboring the vector pCAMIBA1303. Cotyledon explants exposed to an Agrobacterium suspension (OD600 of approximately 0.6) for 10 min and then incubated for 3 days co-cultivation in Murashige and Skoog medium containing an L-cysteine + AgNO3 combination exhibited the highest β-glucuronidase (GUS) expression (94%) and explant regeneration efficiency (76%). After 3 days, the cotyledon explants were subjected to three selection cycles with gradually increasing hygromycin B concentrations (10 to 12 mg/L). The incorporation and expression of hptII in T0 transformed plants were verified by polymerase chain reaction and Southern blot analyses. These transgenic plants (T0) were fertile and morphologically normal. Using the present protocol, a successful transformation efficiency of 14% was achieved, and this protocol can be applied for genome editing and functional studies to improve Chinese cabbage traits.

Keyword

참고문헌 (34)

Antony Ceasar S. Ignacimuthu S. ( 2011 ). Agrobacterium -mediated transformation of finger millet ( Eleusine coracana (L.) Gaertn.) using shoot apex explants. Plant Cell Rep. 30 1759 ？ 1770 . 10.1007/s00299-011-1084-0 21584677

상세보기
Bakshi S. Sadhukhan A. Mishra S. Sahoo L. ( 2011 ). Improved Agrobacterium -mediated transformation of cowpea via sonication and vacuum infiltration. Plant Cell Rep. 30 2281 ？ 2292 . 10.1007/s00299-011-1133-8 21853337

상세보기
Baskar V. Gangadhar B. H. Park S. W. Nile S. H. ( 2016 ). A simple and efficient Agrobacterium tumefaciens -mediated plant transformation of Brassica rapa ssp. pekinensis . 3 Biotech 6 88 ？ 94 .

상세보기
Bhatt R. Asopa P. P. Jain R. Kothari-Chajer A. Kothari S. L. Kachhwaha S. ( 2021 ). Optimization of Agrobacterium mediated genetic transformation in Paspalum scrobiculatum L. (Kodo Millet). Agronomy 11 1104 ？ 1118 . 10.3390/agronomy11061104

상세보기
Burnett L. A. M. Yarrow S. Huang B. ( 1994 ). Enhancement of shoot regeneration from cotyledon explants of Brassica rapa ssp. oleifera through pretreatment with auxin and cytokinin and use of ethylene inhibitors. Plant Cell Tissue Organ. Cult. 37 253 ？ 256 .

상세보기
Cho H. S. Cao J. Ren J. P. Earle E. D. ( 2001 ). Control of Lepidopteran insect pests in transgenic Chinese cabbage ( Brassica rapa ssp. pekinensis ) transformed with a synthetic Bacillus thuringiensis cry1C gene. Plant Cell Rep. 20 1 ？ 7 . 10.1007/s002990000278 30759906

상세보기
Dan Y. ( 2008 ). Biological functions of antioxidants in plant transformation. In Vitro Cell. Dev. Biol.Plant 44 149 ？ 161 . 10.1007/s11627-008-9110-9

상세보기
Enriquez-Obregon G. A. Prieto-Samsonov D. L. De La Riva G. A. Perez M. Selman-Housein G. Vazquez-Padron R. I. ( 1999 ). Agrobacterium -mediated Japonica rice transformation: a procedure assisted by an antinecrotic treatment. Plant Cell Tissue Organ. Cult. 59 159 ？ 168 .
Frame B. R. Shou H. Chikwamba R. K. Zhang Z. Xiang C. Fonger T. M. ( 2002 ). Agrobacterium tumefaciens -mediated transformation of maize embryos using a standard binary vector system. Plant Physiol. 129 13 ？ 22 . 10.1104/pp.000653 12011333

상세보기
Heath M. C. ( 2000 ). Hypersensitive response-related death. Plant Mol. Biol. 44 321 ？ 334 . 10.1023/A:1026592509060 11199391

상세보기
Jefferson R. A. Kavanagh T. A. Bevan M. W. ( 1987 ). GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6 3901 ？ 3907 . 10.1002/j.1460-2075.1987.tb02730.x 3327686

상세보기
Jun S. Kwon S. Y. Pack K. Y. Paek K. H. ( 1995 ). Agrobacterium -mediated transformation and regeneration of fertile transgenic plants of chinese cabbage ( Brassica campestris ssp. pekinensis cv. ‘spring flavor’). Plant Cell Rep. 14 620 ？ 625 . 10.1007/BF00232725 24194308

상세보기
Karthikeyan A. Pandian S. K. Ramesh M. ( 2011 ). Agrobacterium -mediated transformation of leaf base derived callus tissues of popular indica rice ( Oryza sativa L. sub sp. indica cv. ADT 43). Plant Sci. 181 258 ？ 268 . 10.1016/j.plantsci.2011.05.011 21763536

상세보기
Kim H. S. Park Y. H. Nam H. Lee Y. M. Song K. Choi C. ( 2014 ). Overexpression of the Brassica rapa transcription factor WRKY12 results in reduced soft rot symptoms caused by Pectobacterium carotovorum in Arabidopsis and Chinese cabbage. Plant Biol. 16 973 ？ 981 . 10.1111/plb.12149 24552622

상세보기
Konagaya K. I. Tsuda M. Okuzaki A. Ando S. Tabei Y. ( 2013 ). Application of the acetolactate synthase gene as a cisgenic selectable marker for Agrobacterium -mediated transformation in Chinese cabbage ( Brassica rapa ssp. pekinensis ). Plant Biotechnol. 30 125 ？ 133 . 10.5511/plantbiotechnology.13.0124a

상세보기
Ku J. J. Park Y. H. Park Y. D. ( 2006 ). A non-antibiotic selection system uses the phosphomannose-isomerase ( PMI ) gene for Agrobacterium -mediated transformation of Chinese cabbage. J. Plant Biol. 49 115 ？ 122 . 10.1007/BF03031007

원문보기 상세보기
Kuta D. D. Tripathi L. ( 2005 ). Agrobacterium -induced hypersensitive necrotic reaction in plant cells: a resistance response against Agrobacterium -mediated DNA transfer. Afr. J. Biotechnol. 4 752 ？ 757 .
Lee M. K. Kim H. S. Kim J. S. Kim S. H. Park Y. D. ( 2004 ). Agrobacterium -mediated transformation system for large-scale producion of transgenic chinese cabbage ( Brassica rapa L. ssp. pekinensis ) plants for insertional mutagenesis. J. Plant Biol. 47 300 ？ 306 . 10.1007/BF03030544

원문보기 상세보기
Li G. Yue L. Li F. Zhang S. Zhang H. Qian W. ( 2018 ). Research Progress on Agrobacterium tumefaciens -based Transgenic Technology in Brassica rapa . Hortic. Plant J. 4 126 ？ 132 . 10.1016/j.hpj.2018.03.006

상세보기
Li X. Li H. Zhao Y. Zong P. Zhan Z. Piao Z. ( 2021 ). Establishment of a simple and efficient Agrobacterium -mediated genetic transformation system to Chinese Cabbage ( Brassica rapa L. ssp. pekinensis ). Hortic. Plant J. 7 117 ？ 128 . 10.1016/j.hpj.2021.01.006

상세보기
Liu W. Yang Y. Liu Q. ( 2018 ). Establishment of an efficient regeneration system using heading leaves of Chinese cabbage ( Brassica rapa L.) and its application in genetic transformation. Hortic. Environ. Biotechnol. 59 583 ？ 596 . 10.1007/s13580-018-0064-5

상세보기
Liu Y. Zhang Z. Fu J. Wang G. Wang J. Liu Y. ( 2017 ). Transcriptome analysis of Maize immature embryos reveals the roles of cysteine in improving Agrobacterium infection efficiency. Front. Plant Sci. 8 : 1778 . 10.3389/fpls.2017.01778 29089955

상세보기
Min B. W. Cho Y. N. Song M. J. Noh T. K. Kim B. K. Chae W. K. ( 2007 ). Successful genetic transformation of Chinese cabbage using phosphomannose isomerase as a selection marker. Plant Cell Rep. 26 337 ？ 344 . 10.1007/s00299-006-0247-x 17021847

상세보기
Mukhopadhyay A. Arumugam N. Nandakumar P. B. A. Pradhan A. K. Gupta V. Pental D. ( 1992 ). Agrobacterium -mediated genetic transformation of oilseed Brassica campestris : Transformation frequency is strongly influenced by the mode of shoot regeneration. Plant Cell Rep. 11 506 ？ 513 . 10.1007/BF00236266 24213158

상세보기
Murashige T. Skoog F. ( 1962 ). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15 473 ？ 497 . 10.1111/j.1399-3054.1962.tb08052.x

상세보기
Olhoft P. Lin K. Galbraith J. Nielsen N. Somers D. ( 2001 ). The role of thiol compounds in increasing Agrobacterium -mediated transformation of soybean cotyledonary-node cells. Plant Cell Rep. 20 731 ？ 737 . 10.1007/s002990100388

상세보기
Sambrook J. Russell D. W. ( 2001 ). Molecular Cloning: A Laboratory Manual. New York, NY : Cold Spring Harbor Press .
Sgamma T. Thomas B. Muleo R. ( 2015 ). Ethylene inhibitor silver nitrate enhances regeneration and genetic transformation of Prunus avium (L.) cv Stella. Plant Cell Tissue Organ Cult. 120 79 ？ 88 . 10.1007/s11240-014-0581-6

상세보기
Sivanandhan G. Arunachalam C. Vasudevan V. Kapildev G. Sulaiman A. A. Selvaraj N. ( 2016 ). Factors affecting Agrobacterium -mediated transformation in Hybanthus enneaspermus (L.) F. Muell. Plant Biotechnol. Rep. 10 49 ？ 60 . 10.1007/s11816-016-0385-8

상세보기
Sivanandhan G. Choi S. B. Moon J. Choi S. R. Kim S. G. Park Y. D. ( 2019 ). High frequency in vitro regeneration of Chinese cabbage (cv. Kenshin) from hypocotyl and cotyledon Explants. Hortic. Sci.Technol. 37 640 ？ 650 .
Sivanandhan G. Kapil Dev G. Theboral J. Selvaraj N. Ganapathi A. Manickavasagam M. ( 2015 ). Sonication, vacuum infiltration and thiol compounds enhance the Agrobacterium -mediated transformation frequency of Withania somnifera (L.) Dunal. PLoS One 10 : e0124693 . 10.1371/journal.pone.0124693 25927703

상세보기
Vanjildorj E. Song S. Y. Yang Z. H. Choi J. E. Noh Y. S. Park S. ( 2009 ). Enhancement of tolerance to soft rot disease in the transgenic Chinese cabbage ( Brassica rapa L. ssp. pekinensis ) inbred line. Kenshin. Plant Cell Rep. 28 1581 ？ 1591 . 10.1007/s00299-009-0757-4 19680657

상세보기
Velasco P. Rodriguez V. M. Francisco M. Cartea M. E. Soengas P. ( 2017 ). “ Genetics and Breeding of Brassica Crops ,” in Glucosinolates , eds Merillon J. M. Ramawat K. G. ( Cham : Springer International Publishing ), 61 ？ 86 . 10.1007/978-3-319-25462-3_2
Zhang F. L. Takahata Y. Watanabe M. Xu J. B. ( 2000 ). Agrobacterium -mediated transformation of cotyledonary explants of Chinese cabbage ( Brassica campestris L. ssp. pekinensis ). Plant Cell Rep. 19 569 ？ 575 . 10.1007/s002990050775 30754819

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증