[논문]Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating

Hwang, Young Eun; Im, Seonghun; Kim, Hyun; Sohn, Jung-Hoon; Cho, Byung-Kwan; Cho, Ju Hyun; Sung, Bong Hyun; Kim, Sun Chang

doi:10.3390/ijms222111915

[해외논문] Adhesive Antimicrobial Peptides Containing 3,4-Dihydroxy-L-Phenylalanine Residues for Direct One-Step Surface Coating 원문보기

International journal of molecular sciences, v.22 no.21, 2021년, pp.11915 -

Hwang, Young Eun (Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea) , Im, Seonghun (s0ag@kaist.ac.kr (Y.E.H.)) , Kim, Hyun (bcho@kaist.ac.kr (B.-K.C.)) , Sohn, Jung-Hoon (Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea) , Cho, Byung-Kwan (lsh01573@gmail.com (S.I.)) , Cho, Ju Hyun (sohn4090@kribb.re.kr (J.-H.S.)) , Sung, Bong Hyun (Division of Applied Life Science (BK21Four), Research Institute of Life Sciences, Gyeongsang National University, Jinju 52828, Korea) , Kim, Sun Chang (hyun.kim@gnu.ac.kr (H.K.))

Abstract ▼ AI-Helper

Bacterial colonization and transmission via surfaces increase the risk of infection. In this study, we design and employ novel adhesive antimicrobial peptides to prevent bacterial contamination of surfaces. Repeats of 3,4-dihydroxy-L-phenylalanine (DOPA) were added to the C-terminus of NKC, a potent synthetic antimicrobial peptide, and the adhesiveness and antibacterial properties of the resulting peptides are evaluated. The peptide is successfully immobilized on polystyrene, titanium, and polydimethylsiloxane surfaces within 10 min in a one-step coating process with no prior surface functionalization. The antibacterial effectiveness of the NKC-DOPA5-coated polystyrene, titanium, and polydimethylsiloxane surfaces is confirmed by complete inhibition of the growth of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus within 2 h. The stability of the peptide coated on the substrate surface is maintained for 84 days, as confirmed by its bactericidal activity. Additionally, the NKC-DOPA5-coated polystyrene, titanium, and polydimethylsiloxane surfaces show no cytotoxicity toward the human keratinocyte cell line HaCaT. The antimicrobial properties of the peptide-coated surfaces are confirmed in a subcutaneous implantation animal model. The adhesive antimicrobial peptide developed in this study exhibits potential as an antimicrobial surface-coating agent for efficiently killing a broad spectrum of bacteria on contact.

Keyword

참고문헌 (41)

1. Hancock R.E.W. Sahl H.G. Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies Nat. Biotechnol. 2006 24 1551 1557 10.1038/nbt1267 17160061

상세보기
2. Lai Y. Gallo R.L. AMPed up immunity: How antimicrobial peptides have multiple roles in immune defense Trends Immunol. 2009 30 131 141 10.1016/j.it.2008.12.003 19217824

상세보기
3. Glukhov E. Stark M. Burrows L.L. Deber C.M. Basis for selectivity of cationic antimicrobial peptides for bacterial versus mammalian membranes J. Biol. Chem. 2005 280 33960 33967 10.1074/jbc.M507042200 16043484

상세보기
4. Yu K. Lo J.C. Yan M. Yang X. Brooks D.E. Hancock R.E. Lange D. Kizhakkedathu J.N. Anti-adhesive antimicrobial peptide coating prevents catheter associated infection in a mouse urinary infection model Biomaterials 2017 116 69 81 10.1016/j.biomaterials.2016.11.047 27914268

상세보기
5. Song B. Zhang E. Han X. Zhu H. Shi Y. Cao Z. Engineering and Application Perspectives on Designing an Antimicrobial Surface ACS Appl. Mater. Interfaces 2020 12 21330 21341 10.1021/acsami.9b19992 32011846

상세보기
6. Konai M.M. Bhattacharjee B. Ghosh S. Haldar J. Recent Progress in Polymer Research to Tackle Infections and Antimicrobial Resistance Biomacromolecules 2018 19 1888 1917 10.1021/acs.biomac.8b00458 29718664

상세보기
7. Vreuls C. Zocchi G. Garitte G. Archambeau C. Martial J. Van de Weerdt C. Biomolecules in multilayer film for antimicrobial and easy-cleaning stainless steel surface applications Biofouling 2010 26 645 656 10.1080/08927014.2010.506678 20645194

상세보기
8. Shi J. Liu Y. Wang Y. Zhang J. Zhao S.F. Yang G.L. Biological and immunotoxicity evaluation of antimicrobial peptide-loaded coatings using a layer-by-layer process on titanium Sci. Rep. 2015 5 16336 10.1038/srep16336 26548760

상세보기
9. Saha A. Nir S. Reches M. Amphiphilic Peptide with Dual Functionality Resists Biofouling Langmuir 2020 36 4201 4206 10.1021/acs.langmuir.9b03997 32192338

상세보기
10. Parreira P. Monteiro C. Graca V. Gomes J. Maia S. Gomes P. Goncalves I.C. Martins M.C.L. Surface Grafted MSI-78A Antimicrobial Peptide has High Potential for Gastric Infection Management Sci. Rep. 2019 9 18212 10.1038/s41598-019-53918-4 31796755

상세보기
11. He J.C. Chen J.J. Hu G.S. Wang L. Zheng J. Zhan J.Z. Zhu Y.C. Zhong C.T. Shi X.T. Liu S. Immobilization of an antimicrobial peptide on silicon surface with stable activity by click chemistry J. Mater. Chem. B 2018 6 68 74 10.1039/C7TB02557B 32254194

상세보기
12. Godoy-Gallardo M. Mas-Moruno C. Yu K. Manero J.M. Gil F.J. Kizhakkedathu J.N. Rodriguez D. Antibacterial Properties of hLf1–11 Peptide onto Titanium Surfaces: A Comparison Study Between Silanization and Surface Initiated Polymerization Biomacromolecules 2015 16 483 496 10.1021/bm501528x 25545728

상세보기
13. Gao G.Z. Lange D. Hilpert K. Kindrachuk J. Zou Y.Q. Cheng J.T.J. Kazemzadeh-Narbat M. Yu K. Wang R.Z. Straus S.K. The biocompatibility and biofilm resistance of implant coatings based on hydrophilic polymer brushes conjugated with antimicrobial peptides Biomaterials 2011 32 3899 3909 10.1016/j.biomaterials.2011.02.013 21377727

상세보기
14. Wei T. Zhan W.J. Cao L.M. Hu C.M. Qu Y.C. Yu Q. Chen H. Multifunctional and Regenerable Antibacterial Surfaces Fabricated by a Universal Strategy ACS Appl. Mater. Interfaces 2016 8 30048 30057 10.1021/acsami.6b11187 27759376

상세보기
15. Li M. Mitra D. Kang E.T. Lau T. Chiong E. Neoh K.G. Thiol-ol Chemistry for Grafting of Natural Polymers to Form Highly Stable and Efficacious Antibacterial Coatings ACS Appl. Mater. Int. 2017 9 1847 1857 10.1021/acsami.6b10240 27991755

상세보기
16. Qi H.S. Zhang C. Guo H.S. Zheng W.W. Yang J. Zhou X. Zhang L. Bioinspired Multifunctional Protein Coating for Antifogging, Self-Cleaning, and Antimicrobial Properties ACS Appl. Mater. Int. 2019 11 24504 24511 10.1021/acsami.9b03522

상세보기
17. Sundaram H.S. Han X. Nowinski A.K. Ella-Menye J.R. Wimbish C. Marek P. Senecal K. Jiang S.Y. One-Step Dip Coating of Zwitterionic Sulfobetaine Polymers on Hydrophobic and Hydrophilic Surfaces ACS Appl. Mater. Interfaces 2014 6 6664 6671 10.1021/am500362k 24730392

상세보기
18. Cheng H. Yue K. Kazemzadeh-Narbat M. Liu Y.H. Khalilpour A. Li B.Y. Zhang Y.S. Annabi N. Khademhosseini A. Mussel-Inspired Multifunctional Hydrogel Coating for Prevention of Infections and Enhanced Osteogenesis ACS Appl. Mater. Interfaces 2017 9 11428 11439 10.1021/acsami.6b16779 28140564

상세보기
19. Li Y.R. Qin M. Li Y. Cao Y. Wang W. Single Molecule Evidence for the Adaptive Binding of DOPA to Different Wet Surfaces Langmuir 2014 30 4358 4366 10.1021/la501189n 24716607

상세보기
20. Lyu Q. Hsueh N. Chai C.L.L. The Chemistry of Bioinspired Catechol(amine)-Based Coatings ACS Biomater. Sci. Eng. 2019 5 2708 2724 10.1021/acsbiomaterials.9b00281 33405603

상세보기
21. Lim K.Y. Chua R.R.Y. Bow H. Tambyah P.A. Hadinoto K. Leong S.S.J. Development of a catheter functionalized by a polydopamine peptide coating with antimicrobial and antibiofilm properties Acta Biomater. 2015 15 127 138 10.1016/j.actbio.2014.12.015 25541344

상세보기
22. Kim D.J. Lee Y.W. Park M.K. Shin J.R. Lim K.J. Cho J.H. Kim S.C. Efficacy of the designer antimicrobial peptide SHAP1 in wound healing and wound infection (vol 46, pg 2333, 2014) Amino Acids 2014 46 2345 10.1007/s00726-014-1804-1

상세보기
23. Yang K.S. Sung B.H. Park M.K. Lee J.H. Lim K.J. Park S.C. Kim S.L. Kim H.K. Sohn J.H. Kim H.M. Recombinant lipase engineered with amphipathic and coiled-coil peptides ACS Catal. 2015 5 5016 5025 10.1021/cs502079g

상세보기
24. Park I.Y. Cho J.H. Kim K.S. Kim Y.B. Kim M.S. Kim S.C. Helix stability confers salt resistance upon helical antimicrobial peptides J. Biol. Chem. 2004 279 13896 13901 10.1074/jbc.M311418200 14718539

상세보기
25. Chen X. Gao Y. Wang Y. Pan G. Mussel-inspired peptide mimicking: An emerging strategy for surface bioengineering of medical implants Smart Mater. Med. 2021 2 299 304 10.1016/j.smaim.2020.10.005

상세보기
26. Zhi Z.L. Su Y.J. Xi Y.W. Tian L. Xu M. Wang Q.Q. Padidan S. Li P. Huang W. Dual-Functional Polyethylene Glycol-b-polyhexanide Surface Coating with in Vitro and in Vivo Antimicrobial and Antifouling Activities ACS Appl. Mater. Interfaces 2017 9 10383 10397 10.1021/acsami.6b12979 28263055

상세보기
27. Riordan L. Smith E.F. Mills S. Hudson J. Stapley S. Nikoi N.D. Edmondson S. Blair J. Peacock A.F.A. Scurr D. Directly bonding antimicrobial peptide mimics to steel and the real world applications of these materials Mat. Sci. Eng. C-Mater. 2019 102 299 304 10.1016/j.msec.2019.03.064

상세보기
28. Valiei A. Okshevsky M. Lin N. Tufenkji N. Anodized Aluminum with Nanoholes Impregnated with Quaternary Ammonium Compounds Can Kill Pathogenic Bacteria within Seconds of Contact ACS Appl. Mater. Interfaces 2018 10 41207 41214 10.1021/acsami.8b17634 30395430

상세보기
29. Mitra D. Li M. Kang E.T. Neoh K.G. Transparent Copper-Based Antibacterial Coatings with Enhanced Efficacy against Pseudomonas aeruginosa ACS Appl. Mater. Interfaces 2019 11 73 83 10.1021/acsami.8b09640 30525426

상세보기
30. Zhu X.Y. Tang L. Wee K.H. Zhao Y.H. Bai R.B. Immobilization of silver in polypropylene membrane for anti-biofouling performance Biofouling 2011 27 773 786 10.1080/08927014.2011.603830 21781019

상세보기
31. Chen J.X. Howell C. Haller C.A. Patel M.S. Ayala P. Moravec K.A. Dai E.B. Liu L.Y. Sotiri I. Aizenberg M. An immobilized liquid interface prevents device associated bacterial infection in vivo Biomaterials 2017 113 80 92 10.1016/j.biomaterials.2016.09.028 27810644

상세보기
32. Bowler P.G. Duerden B.I. Armstrong D.G. Wound microbiology and associated approaches to wound management Clin. Microbiol. Rev. 2001 14 244 269 10.1128/CMR.14.2.244-269.2001 11292638

상세보기
33. Lajoie M.J. Rovner A.J. Goodman D.B. Aerni H.R. Haimovich A.D. Kuznetsov G. Mercer J.A. Wang H.H. Carr P.A. Mosberg J.A. Genomically recoded organisms expand biological functions Science 2013 342 357 360 10.1126/science.1241459 24136966

상세보기
34. Fredens J. Wang K. de la Torre D. Funke L.F.H. Robertson W.E. Christova Y. Chia T. Schmied W.H. Dunkelmann D.L. Beranek V. Total synthesis of Escherichia coli with a recoded genome Nature 2019 569 514 518 10.1038/s41586-019-1192-5 31092918

상세보기
35. Wiegand I. Hilpert K. Hancock R.E. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances Nat. Protoc. 2008 3 163 175 10.1038/nprot.2007.521 18274517

상세보기
36. Shin Y.M. Jun I. Lim Y.M. Rhim T. Shin H. Bio-inspired Immobilization of Cell-Adhesive Ligands on Electrospun Nanofibrous Patches for Cell Delivery Macromol. Mater. Eng. 2013 298 555 564 10.1002/mame.201200217

상세보기
37. Alves D. Pereira M.O. Bio-Inspired Coating Strategies for the Immobilization of Polymyxins to Generate Contact-Killing Surfaces Macromol. Biosci. 2016 16 1450 1460 10.1002/mabi.201600122 27345452

상세보기
38. Chen X.Y. Gao C.Y. Influences of surface coating of PLGA nanoparticles on immune activation of macrophages J. Mater. Chem. B 2018 6 2065 2077 10.1039/C7TB03080K 32254430

상세보기
39. He S. Zhou P. Wang L.X. Xiong X.L. Zhang Y.F. Deng Y. Wei S.C. Antibiotic-decorated titanium with enhanced antibacterial activity through adhesive polydopamine for dental/bone implant J. R. Soc. Interface 2014 11 20140169 10.1098/rsif.2014.0169 24647910

상세보기
40. Li X.Y. Gao P. Tan J.Y. Xiong K.Q. Maitz M.F. Pan C.J. Wu H.K. Chen Y. Yang Z.L. Huang N. Assembly of metal phenolic/catecholamine networks for synergistically anti-inflammatory, antimicrobial, and anticoagulant coatings ACS Appl. Mater. Interfaces 2018 10 40844 40853 10.1021/acsami.8b14409 30403339

상세보기
41. Chen R.X. Willcox M.D.P. Ho K.K.K. Smyth D. Kumar N. Antimicrobial peptide melimine coating for titanium and its in vivo antibacterial activity in rodent subcutaneous infection models Biomaterials 2016 85 142 151 10.1016/j.biomaterials.2016.01.063 26871890

상세보기

LOADING...

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증