[논문]Trichomonas vaginalis induces apoptosis via ROS and ER stress response through ER–mitochondria crosstalk in SiHa cells

Gao, Fei Fei; Quan, Juan-Hua; Lee, Min A.; Ye, Wei; Yuk, Jae-Min; Cha, Guang-Ho; Choi, In-Wook; Lee, Young-Ha

doi:10.1186/s13071-021-05098-2

[해외논문] Trichomonas vaginalis induces apoptosis via ROS and ER stress response through ER–mitochondria crosstalk in SiHa cells 원문보기

Parasites & vectors, v.14 no.1, 2021년, pp.603 -

Gao, Fei Fei (Brain Korea 21 FOUR Project for Medical Science, Chungnam National University College of Medicine, Daejeon, 35015 Korea) , Quan, Juan-Hua (Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001 China) , Lee, Min A. (Department of Obstetrics and Gynecology, Chungnam National University, DeaJeon, 35015 Korea) , Ye, Wei (Department of Obstetrics and Gynecology, The Affiliated Hospital of Guangdong Medical University, Zhanjiang, 524001 China) , Yuk, Jae-Min (Brain Korea 21 FOUR Project for Medical Science, Chungnam National University College of Medicine, Daejeon, 35015 Korea) , Cha, Guang-Ho (Department of Medical Science and Department of Infection Biology, Chungnam National University College of Medicine, 6 Munhwa-dong, Jung-gu, Daejeon, 35015 Korea) , Choi, In-Wook (Department of Medical Science and Department of Infection Biology, Chungnam National University College of Medicine, 6 Munhwa-dong, Jung-gu, Daejeon, 35015 Korea) , Lee, Young-Ha (Brain Korea 21 FOUR Project for Medical Science, Chungnam National University College of Medici)

Abstract ▼ AI-Helper

BackgroundTrichomonas vaginalis causes lesions on the cervicovaginal mucosa in women; however, its pathogenesis remains unclear. We have investigated the involvement of the endoplasmic reticulum (ER) in the induction of apoptosis by T. vaginalis and its molecular mechanisms in human cervical cancer SiHa cells.MethodsApoptosis, reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP), ER stress response and Bcl-2 family protein expression were evaluated using immunocytochemistry, flow cytometry, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide dye staining and western blotting.ResultsTrichomonas vaginalis induced mitochondrial ROS production, apoptosis, the ER stress response and mitochondrial dysfunction, such as MMP depolarization and an imbalance in Bcl-2 family proteins, in SiHa cells in a parasite burden- and infection time-dependent manner. Pretreatment with N-acetyl cysteine (ROS scavenger) or 4-phenylbutyric acid (4-PBA; ER stress inhibitor) significantly alleviated apoptosis, mitochondrial ROS production, mitochondrial dysfunction and ER stress response in a dose-dependent manner. In addition, T. vaginalis induced the phosphorylation of apoptosis signal regulating kinase 1 (ASK1) and c-Jun N-terminal kinases (JNK) in SiHa cells, whereas 4-PBA or SP600125 (JNK inhibitor) pretreatment significantly attenuated ASK1/JNK phosphorylation, mitochondrial dysfunction, apoptosis and ER stress response in SiHa cells, in a dose-dependent manner. Furthermore, T. vaginalis excretory/secretory products also induced mitochondrial ROS production, apoptosis and the ER stress response in SiHa cells, in a time-dependent manner.ConclusionsTrichomonas vaginalis induces apoptosis through mitochondrial ROS and ER stress responses, and also promotes ER stress-mediated mitochondrial apoptosis via the IRE1/ASK1/JNK/Bcl-2 family protein pathways in SiHa cells. These data suggest that T. vaginalis-induced apoptosis is affected by ROS and ER stress response via ER–mitochondria crosstalk.Graphical AbstractSupplementary InformationThe online version contains supplementary material available at 10.1186/s13071-021-05098-2.

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