[논문]Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes

Chandel, Ankush; Mann, Ross; Kaur, Jatinder; Norton, Sally; Edwards, Jacqueline; Spangenberg, German; Sawbridge, Timothy

doi:10.3389/fmicb.2021.784796

Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes 원문보기

Frontiers in microbiology, v.12, 2021년, pp.784796 -

Chandel, Ankush (Agriculture Victoria Research, AgriBio, Centre for AgriBioscience , Bundoora, VIC , Australia) , Mann, Ross (Agriculture Victoria Research, AgriBio, Centre for AgriBioscience , Bundoora, VIC , Australia) , Kaur, Jatinder (Agriculture Victoria Research, AgriBio, Centre for AgriBioscience , Bundoora, VIC , Australia) , Norton, Sally (Agriculture Victoria Research, Australian Grains Genebank , Horsham, VIC , Australia) , Edwards, Jacqueline (Agriculture Victoria Research, AgriBio, Centre for AgriBioscience , Bundoora, VIC , Australia) , Spangenberg, German (Agriculture Victoria Research, AgriBio, Centre for AgriBioscience , Bundoora, VIC , Australia) , Sawbridge, Timothy (Agriculture Victoria Research, AgriBio, Centre for AgriBioscience , Bundoora, VIC , Australia)

Abstract ▼ AI-Helper

Global seed vaults are important, as they conserve plant genetic resources for future breeding to improve crop yield and quality and to overcome biotic and abiotic stresses. However, little is known about the impact of standard storage procedures, such as seed drying and cold storage on the seed bacterial community, and the ability to recover seed-associated bacteria after storage. In this study, soybean [Glycine max (L.) Merr.] seeds were analyzed to characterize changes in the bacterial community composition and culturability under varying storage conditions. The G. max bacterial microbiome was analyzed from undried seed, dried seed, and seed stored for 0, 3, 6, and 14months. Storage temperatures consisted of −20°C, 4°C, and room temperature (RT), with −20°C being commonly used in seed storage vaults globally. The seed microbiome of G. max was dominated by Gammaproteobacteria under all conditions. Undried seed was dominated by Pantoea (33.9%) and Pseudomonas (51.1%); however, following drying, the abundance of Pseudomonas declined significantly (0.9%), Pantoea increased significantly (73.6%), and four genera previously identified including Pajaroellobacter, Nesterenkonia, env.OPS_17, and Acidibacter were undetectable. Subsequent storage at RT, 4, or −20°C maintained high-abundance Genera at the majority of time points, although RT caused greater fluctuations in abundances. For many of the low-abundance Genera, storage at −20°C resulted in their gradual disappearance, whereas storage at 4°C or RT resulted in their more rapid disappearance. The changes in seed bacterial composition were reflected by cultured bacterial taxa obtained from the stored G. max seed. The main taxa were largely culturable and had similar relative abundance, while many, but not all, of the low-abundance taxa were also culturable. Overall, these results indicate that the initial seed drying affects the seed bacterial composition, suggesting that microbial isolation prior to seed drying is recommended to conserve these microbes. The standard seed storage condition of −20°C is most suitable for conservation of the bacterial seed microbiome, as this storage temperature slows down the loss of seed bacterial diversity over longer time periods, particularly low-abundance taxa.

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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Implications of Seed Vault Storage Strategies for Conservation of Seed Bacterial Microbiomes 원문보기

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