[논문]Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip

Roh, Hyewon; Kim, Hwisoo; Park, Je-Kyun

doi:10.3390/bios11120506

[해외논문] Construction of a Fibroblast-Associated Tumor Spheroid Model Based on a Collagen Drop Array Chip 원문보기

Biosensors, v.11 no.12, 2021년, pp.506 -

Roh, Hyewon (Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea) , Kim, Hwisoo (hyewonr@kaist.ac.kr (H.R.)) , Park, Je-Kyun (hwiss@kaist.ac.kr (H.K.))

Abstract ▼ AI-Helper

Spheroid, a 3D aggregate of tumor cells in a spherical shape, has overcome the limitations of conventional 3D cell models to accurately mimic the in-vivo environment of a human body. The spheroids are cultured with other primary cells and embedded in collagen drops using hang drop plates and low-attachment well plates to construct a spheroid–hydrogel model that better mimics the cell–cell and cell–extracellular matrix (ECM) interactions. However, the conventional methods of culturing and embedding spheroids into ECM have several shortcomings. The procedure of transferring a single spheroid at a time by manual pipetting results in well-to-well variation and even loss or damage of the spheroid. Based on the previously introduced droplet contact-based spheroid transfer technique, we present a poly(dimethylsiloxane) and resin-based drop array chip and a pillar array chip with alignment stoppers, which enhances the alignment between the chips for uniform placement of spheroids. This method allows the facile and stable transfer of the spheroid array and even eliminates the need for a stereomicroscope while handling the cell models. The novel platform demonstrates a homogeneous and time-efficient construction and diverse analysis of an array of fibroblast-associated glioblastoma multiforme spheroids that are embedded in collagen.

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참고문헌 (30)

1. Yamada K.M. Cukierman E. Modeling tissue morphogenesis and cancer in 3D Cell 2007 130 601 610 10.1016/j.cell.2007.08.006 17719539

상세보기
2. Swartz M.A. Iida N. Roberts E.W. Sangaletti S. Wong M.H. Yull F.E. Coussens L.M. DeClerck Y.A. Tumor microenvironment complexity: Emerging roles in cancer therapy Cancer Res. 2012 72 2473 2480 10.1158/0008-5472.CAN-12-0122 22414581

상세보기
3. Ko J. Ahn J. Kim S. Lee Y. Lee J. Park D. Jeon N.L. Tumor spheroid-on-a-chip: A standardized microfluidic culture platform for investigating tumor angiogenesis Lab Chip 2019 19 2822 2833 10.1039/C9LC00140A 31360969

상세보기
4. Huh D. Hamilton G.A. Ingber D.E. From 3D cell culture to organs-on-chips Trends Cell Biol. 2011 21 745 754 10.1016/j.tcb.2011.09.005 22033488

상세보기
5. Ong S.M. Zhang C. Toh Y.C. Kim S.H. Foo H.L. Tan C.H. van Noort D. Park S. Yu H. A gel-free 3D microfluidic cell culture system Biomaterials 2008 29 3237 3244 10.1016/j.biomaterials.2008.04.022 18455231

상세보기
6. Loessner D. Stok K.S. Lutolf M.P. Hutmacher D.W. Clements J.A. Rizzi S.C. Bioengineered 3D platform to explore cell-ECM interactions and drug resistance of epithelial ovarian cancer cells Biomaterials 2010 31 8494 8506 10.1016/j.biomaterials.2010.07.064 20709389

상세보기
7. Kim M.S. Yeon J.H. Park J.-K. A microfluidic platform for 3-dimensional cell culture and cell-based assays Biomed. Microdevices 2007 9 25 34 10.1007/s10544-006-9016-4 17103048

상세보기
8. Fennema E. Rivron N. Rouwkema J. van Blitterswijk C. de Boer J. Spheroid culture as a tool for creating 3D complex tissues Trends Biotechnol. 2013 31 108 115 10.1016/j.tibtech.2012.12.003 23336996

상세보기
9. Monteiro M.V. Gaspar V.M. Ferreira L.P. Mano J.F. Hydrogel 3D In vitro tumor models for screening cell aggregation mediated drug response Biomater. Sci. 2020 8 1855 1864 10.1039/C9BM02075F 32091033

상세보기
10. Mehta G. Hsiao A.Y. Ingram M. Luker G.D. Takayama S. Opportunities and challenges for use of tumor spheroids as models to test drug delivery and efficacy J. Control. Release 2012 164 192 204 10.1016/j.jconrel.2012.04.045 22613880

상세보기
11. Lee J.M. Park D.Y. Yang L. Kim E.J. Ahrberg C.D. Lee K.B. Chung B.G. Generation of uniform-sized multicellular tumor spheroids using hydrogel microwells for advanced drug screening Sci. Rep. 2018 8 17145 10.1038/s41598-018-35216-7 30464248

상세보기
12. Charoen K.M. Fallica B. Colson Y.L. Zaman M.H. Grinstaff M.W. Embedded multicellular spheroids as a biomimetic 3D cancer model for evaluating drug and drug-device combinations Biomaterials 2014 35 2264 2271 10.1016/j.biomaterials.2013.11.038 24360576

상세보기
13. Truong H.H. de Sonneville J. Ghotra V.P.S. Xiong J. Price L. Hogendoorn P.C.W. Spaink H.H. van de Water B. Danen E.H.J. Automated microinjection of cell-polymer suspensions in 3D ECM scaffolds for high-throughput quantitative cancer invasion screens Biomaterials 2012 33 181 188 10.1016/j.biomaterials.2011.09.049 22018386

상세보기
14. Jeong S.Y. Lee J.H. Shin Y. Chung S. Kuh H.J. Co-culture of tumor spheroids and fibroblasts in a collagen matrix-incorporated microfluidic chip mimics reciprocal activation in solid tumor microenvironment PLoS ONE 2016 11 e0159013 10.1371/journal.pone.0159013 27391808

상세보기
15. Chaudhuri O. Koshy S.T. Da Cunha C.B. Shin J.W. Verbeke C.S. Allison K.H. Mooney D.J. Extracellular matrix stiffness and composition jointly regulate the induction of malignant phenotypes in mammary epithelium Nat. Mater. 2014 13 970 978 10.1038/nmat4009 24930031

상세보기
16. Weaver V.M. Petersen O.W. Wang F. Larabell C.A. Briand P. Damsky C. Bissell M.J. Reversion of the malignant phenotype of human breast cells in three- dimensional culture and in vivo by integrin blocking antibodies J. Cell Biol. 1997 137 231 245 10.1083/jcb.137.1.231 9105051

상세보기
17. Lang S.H. Sharrard R.M. Stark M. Villette J.M. Maitland N.J. Prostate epithelial cell lines form spheroids with evidence of glandular differentiation in three-dimensional Matrigel cultures Br. J. Cancer 2001 85 590 599 10.1054/bjoc.2001.1967 11506501

상세보기
18. Li Y. Kumacheva E. Hydrogel microenvironments for cancer spheroid growth and drug screening Sci. Adv. 2018 4 eaas8998 10.1126/sciadv.aas8998 29719868

상세보기
19. Nashimoto Y. Hayashi T. Kunita I. Nakamasu A. Torisawa Y.S. Nakayama M. Takigawa-Imamura H. Kotera H. Nishiyama K. Miura T. Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device Integr. Biol. 2017 9 506 518 10.1039/C7IB00024C 28561127

상세보기
20. Liao W. Wang J. Xu J. You F. Pan M. Xu X. Weng J. Han X. Li S. Li Y. High-throughput three-dimensional spheroid tumor model using a novel stamp-like tool J. Tissue Eng. 2019 10 204173141988918 10.1177/2041731419889184

상세보기
21. Han C. Takayama S. Park J. Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system Sci. Rep. 2015 5 11891 10.1038/srep11891 26144552

상세보기
22. Kang E. Choi Y.Y. Jun Y. Chung B.G. Lee S.H. Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval Lab Chip 2010 10 2651 2654 10.1039/c0lc00005a 20740239

상세보기
23. Kim H. Cho C.H. Park J.-K. High-throughput culture and embedment of spheroid array using droplet contact-based spheroid transfer Biomicrofluidics 2018 12 044109 10.1063/1.5039965 30867862

상세보기
24. Kim H. Roh H. Kim H. Park J.-K. Droplet contact-based spheroid transfer technique as a multi-step assay tool for spheroid arrays Lab Chip 2021 21 4155 4165 10.1039/D1LC00581B 34515264

상세보기
25. Kelm J.M. Timmins N.E. Brown C.J. Fussenegger M. Nielsen L.K. Method for generation of homogeneous multicellular tumor spheroids applicable to a wide variety of cell types Biotechnol. Bioeng. 2003 83 173 180 10.1002/bit.10655 12768623

상세보기
26. Amaral R.L.F. Miranda M. Marcato P.D. Swiech K. Comparative analysis of 3D bladder tumor spheroids obtained by forced floating and hanging drop methods for drug screening Front. Physiol. 2017 8 605 10.3389/fphys.2017.00605 28878686

상세보기
27. Zhang W. Li C. Baguley B.C. Zhou F. Zhou W. Shaw J.P. Wang Z. Wu Z. Liu J. Optimization of the formation of embedded multicellular spheroids of MCF-7 cells: How to reliably produce a biomimetic 3D model Anal. Biochem. 2016 515 47 54 10.1016/j.ab.2016.10.004 27717854

상세보기
28. Xu X. Farach-Carson M.C. Jia X. Three-dimensional in vitro tumor models for cancer research and drug evaluation Biotechnol. Adv. 2014 32 1256 1268 10.1016/j.biotechadv.2014.07.009 25116894

상세보기
29. Lee S.W. Kwak H.S. Kang M.H. Park Y.Y. Jeong G.S. Fibroblast-associated tumour microenvironment induces vascular structure-networked tumouroid Sci. Rep. 2018 8 2365 10.1038/s41598-018-20886-0 29403007

상세보기
30. Nashimoto Y. Okada R. Hanada S. Arima Y. Nishiyama K. Miura T. Yokokawa R. Vascularized cancer on a chip: The effect of perfusion on growth and drug delivery of tumor spheroid Biomaterials 2020 229 119547 10.1016/j.biomaterials.2019.119547 31710953

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