[논문]Cell Staining

Rodig, Scott J.

doi:10.1101/pdb.top099606

Cell Staining

Cold Spring Harbor protocols, v.2022 no.6, 2022년, pp.pdb.top099606 - pdb.top099606

Rodig, Scott J.

Abstract ▼ AI-Helper

When labeled antibodies are used to detect antigens in cells or tissues, several characteristics of an antigen can be readily determined. Most importantly, cell staining will show both the presence and subcellular localization of an antigen. Double-labeling techniques permit the simultaneous detection of two antigens, allowing comparisons of the relative distribution of different antigens. Many cell-staining methods can also be used in conjunction with conventional histological stains and autoradiographic methods to compare the localization of the antigen with other markers. Cell staining can also be used in pathology studies for determining such variables as the type of infectious organism, the progenitor of a neoplastic cell, or the presence of an inflammatory response. With certain modifications, the procedure can be used to purify cells on the basis of the antigenic composition of their cell surface. Cell staining is a versatile technique and, if the antigen is highly localized, can detect as few as a thousand antigen molecules in a cell or tissue. In some circumstances, cell staining may also be used to determine the approximate concentration of an antigen. Improvements in antibody labeling methods, microscopes, cameras, and image analyzers are rapidly extending the sensitivity of cell-staining procedures and are making these techniques more quantitative. Even without these improvements, cell staining can yield important qualitative and semiquantitative data. This introduction describes protocols for cell staining techniques and includes a discussion of major constraints, antibody selection, and troubleshooting.

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Rodig, Scott J.. Detecting Horseradish Peroxidase-Labeled Cells. Cold Spring Harbor protocols, vol.2019, no.4, pdb.prot099713-pdb.prot099713.

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Rodig, Scott J.. Detecting Alkaline Phosphatase–Labeled Cells. Cold Spring Harbor protocols, vol.2019, no.4, pdb.prot099721-pdb.prot099721.

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Rodig, Scott J.. Detecting Gold-Labeled Cells. Cold Spring Harbor protocols, vol.2019, no.7, pdb.prot099754-pdb.prot099754.

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Rodig, Scott J.. Detecting Iodine-Labeled Cells. Cold Spring Harbor protocols, vol.2019, no.7, pdb.prot099762-pdb.prot099762.

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Rodig, Scott J.. Counterstaining, Mounting, and Photographing Stained Cells. Cold Spring Harbor protocols, vol.2019, no.4, pdb.prot099770-pdb.prot099770.

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Rodig, Scott J.. Growing Adherent Cells for Staining. Cold Spring Harbor protocols, vol.2020, no.8, pdb.prot099614-pdb.prot099614.

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Rodig, Scott J.. Attaching Suspension Cells to Slides for Staining. Cold Spring Harbor protocols, vol.2020, no.12, pdb.prot099622-.

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Rodig, Scott J.. Preparing Cell Smears for Staining. Cold Spring Harbor protocols, vol.2020, no.12, pdb.prot099630-.

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Rodig, Scott J.. Fixing Attached Cells for Staining. Cold Spring Harbor protocols, vol.2020, no.8, pdb.prot099689-pdb.prot099689.

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Rodig, Scott J.. Binding Antibodies to Attached Cells or Tissues in Preparation for Staining. Cold Spring Harbor protocols, vol.2020, no.8, pdb.prot099705-pdb.prot099705.

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Rodig, Scott J.. Detecting β-Galactosidase-Labeled Cells. Cold Spring Harbor protocols, vol.2020, no.5, pdb.prot099739-pdb.prot099739.

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Rodig, Scott J.. Preparation of Yeast Cells for Staining. Cold Spring Harbor protocols, vol.2022, no.6, pdb.prot099648-pdb.prot099648.

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Rodig, Scott J.. Preparing Frozen Tissue Sections for Staining. Cold Spring Harbor protocols, vol.2021, no.3, pdb.prot099655-pdb.prot099655.

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Rodig, Scott J.. Preparing Paraffin Tissue Sections for Staining. Cold Spring Harbor protocols, vol.2021, no.3, pdb.prot099663-pdb.prot099663.

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Rodig, Scott J.. Embedding Cultured Cells in Matrigel for Staining. Cold Spring Harbor protocols, vol.2021, no.3, pdb.prot099671-pdb.prot099671.

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Rodig, Scott J.. Fixing and Binding Antibodies to Suspension Cells in Preparation for Staining. Cold Spring Harbor protocols, vol.2021, no.6, pdb.prot099697-pdb.prot099697.

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Rodig, Scott J.. Detecting Fluorochrome-Labeled Cells. Cold Spring Harbor protocols, vol.2021, no.6, pdb.prot099747-pdb.prot099747.

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Cell Staining

Abstract ▼ AI-Helper

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