[논문]Discovery and validation of metabolite markers in bloodstains for bloodstain age estimation

Lee, Seungyeon; Lee, You-Rim; Lee, Jiyeong; Kang, Hee-Gyoo

doi:10.1039/d3an00603d

Discovery and validation of metabolite markers in bloodstains for bloodstain age estimation

The Analyst : An International Journal of Analytical and Bioanalytical Science, v.148 no.17, 2023년, pp.4180 - 4188

Lee, Seungyeon (Department of Senior Healthcare, Graduate School, Eulji University) , Lee, You-Rim (Department of Senior Healthcare, Graduate School, Eulji University) , Lee, Jiyeong (Department of Biomedical Laboratory Science, College of Health Science, Eulji University) , Kang, Hee-Gyoo (Department of Senior Healthcare, Graduate School, Eulji University)

Abstract ▼ AI-Helper

Bloodstain age estimation involves measuring time-dependent changes in the levels of biomolecules in bloodstains. Although several studies have identified bloodstain metabolites as markers for estimating bloodstain age, none have considered sex, age-related metabolomic differences, or long-time bloodstain age. Therefore, we aimed to identify metabolite markers for estimating the age of bloodstains at weekly intervals within 28 days and validate them through multiple reaction monitoring. Adenosine 5′-monophosphate, choline, and pyroglutamic acid were selected as markers. Seven metabolites were validated, including five previously reported metabolites, ergothioneine, hypoxanthine, L-isoleucine, L-tryptophan, and pyroglutamic acid. Choline and hypoxanthine can be used to differentiate bloodstains between days 0 and 14 after deposition at weekly intervals, whereas L-isoleucine and L-tryptophan can help distinguish bloodstains between 7 days before and 14 days after deposition. Evaluation of the changes in metabolite levels according to sex and age revealed that the average levels of all seven metabolites were higher in women on day 0. Moreover, the level of ergothioneine was significantly higher in elderly individuals than in young individuals at all time points. In this study, we confirmed the potential effectiveness of metabolites in bloodstains as forensic markers and provided a new perspective on metabolomic approaches linked to forensic science.Graphic AbstractThis study offers novel insights into the potential effectiveness of metabolites in bloodstains as forensic markers and provides the basis for the application of metabolomic approaches in forensic science.

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Discovery and validation of metabolite markers in bloodstains for bloodstain age estimation

Abstract ▼ AI-Helper

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