[논문]Cell protection by fructose is independent of adenosine triphosphate (ATP) levels in paracetamol injury to rat liver slices

doi:10.1016/0300-483X(95)03256-F

Cell protection by fructose is independent of adenosine triphosphate (ATP) levels in paracetamol injury to rat liver slices

Toxicology, v.107 no.3, 1996년, pp.177 - 187

Martin, Francis L. (Corresponding author, Haddow Laboratory, Institute of Cancer Research, 15 Cotswold rd., Sutton, Surrey SM2 5NG, UK. Tel.: (+44-181) 643 8901, Ext. 4668) , Mclean, Andre E.M. (Fax: (+44-181) 770 7290.)

Abstract ▼ AI-Helper

AbstractFructose protects cells against several types of injury but the mechanism of protection is uncertain. We have used paracetamol injury in rat liver slices as a model system to investigate the role of ATP levels in protection by fructose. Fructose depletes ATP levels in a concentration-dependent fashion in liver slices obtained from non-induced rats. Liver slices recover their ATP levels in the presence of fructose concentrations up to 10 mM. However, in the presence of 20 mM fructose, ATP levels are depleted for the duration of 240 min incubation. Adenine at 100 μM reverses the ATP depletion induced by 20 mM fructose in slices over 240 min incubation. Liver slices obtained from phenobarbitoneinduced rats were exposed to 10 mM paracetamol for 120 min and, then, incubated without paracetamol, with or without fructose for another 240 min. Introduction of 10 mM or 20 mM fructose in the second stage of incubation prevents paracetamol-induced injury. Fructose at 20 mM induces a rapid and marked depletion in slice ATP levels and these remain low throughout the second 240 min incubation period. Fructose at 10 mM maintains high ATP levels, even in paracetamol-treated slices. There is a profound protective effect against paracetamol-induced injury by either concentration. This suggests that protection is not dependent on high or on low ATP levels. Incubation of paracetamoltreated slices in the presence of 20 mM fructose plus 100 μM adenine in the second 240 min incubation period still results in the same level of protection as with 20 or 10 mM fructose alone while reversing the ATP depletion observed with 20 mM fructose.

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참고문헌 (30)

Clin. Chem. Amador 9 391 1963 10.1093/clinchem/9.4.391 Serum lactic dehydrogenase activity: an analytical assessment of current assays

상세보기
Am. J. Physiol. Anundi 255 G58 1989 Hypoxie liver cell death: critical PO2 and dependence of viability on glycolysis
Am. J. Physiol. Anundi 253 G390 1987 Fructose prevents hypoxic cell death in liver
Biochim. Biophys. Acta. Balaban 804 419 1984 10.1016/0167-4889(84)90069-7 Studies on the relationship between glycolysis and (Na+ + K+)-ATPase in cultured cells

상세보기
Eur. J. Clin. Invest. Bode 3 436 1973 10.1111/j.1365-2362.1973.tb02211.x Depletion of liver adenosine phosphates and metabolic effects of intravenous effects of fructose or sorbitol in man and in the rat

상세보기
J. Biol. Chem. Burcham 266 5049 1991 10.1016/S0021-9258(19)67754-9 Acetaminophen toxicity results in site-specific mitochondrial damage in isolated mouse hepatocytes

상세보기
J. Clin. Invest. Curtis Morris 61 209 1978 10.1172/JCI108920 Evidence that the severity of depletion of inorganic phosphate determines the severity of the disturbance of adenine nucleotide metabolism in the liver and renal cortex of the fructoseloaded rat

상세보기
Biochem. Biophys. Res. Commun. Di Monte 153 734 1988 10.1016/S0006-291X(88)81156-2 Fructose prevents 1-methyl-4-phenyl 1,2,3,6-tetrahydropyridine (MPTP)-induced ATP depletion and toxicity in isolated hepatocytes

상세보기
Nature Farber 203 34 1964 10.1038/203034a0 Biochemical pathology of acute hepatic adenosine triphosphate deficiency

상세보기
Chem.-Biol. Interact. Gooding 20 299 1978 10.1016/0009-2797(78)90108-4 Cytochrome P-450 distribution in rat liver and the effect of sodium phenobarbitone administration

상세보기
J. Biol. Chem. Lowry 193 265 1951 10.1016/S0021-9258(19)52451-6 Protein measurement with the Folin phenol reagent

상세보기
Methods Enzymol. Lundin 133 27 1986 10.1016/0076-6879(86)33053-2 Estimation of biomass in growing cell lines by adenosine triphosphate assay
Biochem. Pharmacol. Marshall 18 153 1969 10.1016/0006-2952(69)90020-3 The effect of oral phenobarbitone on hepatic microsomal cytochrome P-450 and demethylation activity in rats fed normal and low protein diets

상세보기
Nature McLean 185 936 1960 10.1038/185936a0 Vitamin E deficiency and ion transport in rat liver slices

상세보기
Biochem. Pharmacol. McLean 27 425 1975 10.1016/0006-2952(78)90371-4 The effect of diet on the toxicity of paracetamol and the safety of paracetamolmethionine mixtures

상세보기
Biochem. Pharmacol. McLean 27 425 1978 10.1016/0006-2952(78)90371-4 An in vitro model of liver injury using paracetamol treatment of liver slices and prevention of injury by some antioxidants

상세보기
Toxicol. Appl. Pharmacol. Meyers 93 378 1988 10.1016/0041-008X(88)90040-3 Acetaminophen-induced inhibition of hepatic mitochondrial respiration in mice

상세보기
J. Pharmacol. Exp. Ther. Mitchell 187 211 1973 Acetaminophen induced hepatic necrosis.IV. Protective role of glutathione

상세보기
Biochem. Pharmacol. Mourelle 41 2023 1990 10.1016/0006-2952(90)90232-A Electron transport and protection of liver slices in the late stages of paracetamol injury

상세보기
Biochem. Pharmacol. Mourelle 41 1831 1991 10.1016/0006-2952(91)90121-K Prevention of paracetamol-induced liver injury by fructose

상세보기
Biochem. Pharmacol. Nazareth 42 931 1991 10.1016/0006-2952(91)90055-A Effect of paracetamol on mitochondrial membrane function in rat liver slices

상세보기
Lancet Prescott i 519 1971 10.1016/S0140-6736(71)91125-1 Plasma paracetamol half-life and hepatic necrosis in patients with paracetamol overdosage

상세보기
Hum. Toxicol. Prescott 3 75S 1984 10.1177/096032718400300109 Clinical features and management of analgesic poisoning

상세보기
Biochem. Pharmacol. Rosen 32 2053 1983 10.1016/0006-2952(83)90426-4 Acetaminophen hepatotoxicity, an alternative mechanism

상세보기
Arch. Biochem. Biophys. Silva 289 313 1991 10.1016/0003-9861(91)90416-G Prevention of nitrofurantoin-induced cytotoxicity in isolated hepatocytes by fructose

상세보기
Arch. Int. Med. Vale 141 394 1981 10.1001/archinte.141.3.394 Treatment of acetaminophen poisoning, use of oral methionine
Biochem. J. Van Den Berghe 162 601 1977 10.1042/bj1620601 The mechanism of adenosine triphosphate depletion in the liver after a load of fructose

상세보기
J. Biol. Chem. Villa-Trevino 238 1757 1963 10.1016/S0021-9258(18)81133-4 The role of adenosine triphosphate deficiency in ethionine-induced inhibition of protein synthesis

상세보기
Biochem. J. Woods 119 501 1970 10.1042/bj1190501 The cause of hepatic accumulation of fructose.1-phosphate on fructose loading

상세보기
Arch. Biochem. Biophys. Wu 282 358 1990 10.1016/0003-9861(90)90129-M Relationship between the mitochondrial transmembrane potential, ATP concentration, and cytotoxicity in isolated hepatocytes

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Cell protection by fructose is independent of adenosine triphosphate (ATP) levels in paracetamol injury to rat liver slices

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