[논문]Mouse embryo development in media containing growth factors

Menino, A.R.; Williams, J.S.; Gardiner, C.S.; John, T.L.

doi:10.1016/0378-4320(89)90014-6

Mouse embryo development in media containing growth factors

Animal reproduction science, v.18 no.1/3, 1989년, pp.125 - 137

Menino, A.R. , Williams, J.S. , Gardiner, C.S. , John, T.L.

Abstract ▼ AI-Helper

The effects of various growth factors on in vitro mouse embryo development were investigated. Two-cell mouse embryos were cultured in: Whitten's medium with 10 mg/ml bovine serum albumin (WM) as the control, WM with 0.1, 1.0 or 10.0 units/ml T-cell growth factor (TCGF0.1, TCGF1.0, TCGF10.0), WM with 0.012, 0.12 or 1.2 μg/ml marrow cell stimulator (MCS0.012, MCS0.12, MCS1.2), WM with 0.4, 4.0 or 40.0 μl/ml MCS control buffer (MCS0.4, MCS4.0, MCS40.0) and WM with 1, 10 or 100 ng/ml of epidermal growth factor (EGF1, EGF10, EGF100), fibroblast growth factor (FGF1, FGF10, FGF100), endothelial cell growth factor (ECGF1, ECGF10, ECGF100), nerve growth factor 2.5S (NGF2.5S1, NGF2.5S10, NGF2.5S100), nerve growth factor 7S (NGF7S1, NGF7S10, NGF7S100), multiplication stimulating activity (MSA1, MSA10, MSA100) or liver cell growth factor (LCGF1, LCGF10, LCGF100). At 24, 48, 72 and 96 h of culture, 10-12 embryos from each treatment were fixed and stained in hematoxylin and eosin for the determination of cell numbers and the incidences of mitotic figures. Greater numbers of embryos hatched in MSA1, ECGF1, ECGF10 and NGF7S supplemented media than in WM (P < 0.05). Cell division was stimulated in embryos cultured in FGF1 when compared to WM and in MCS1.2 when compared to MCS40.0 (P < 0.05). Enhancement of hatching in MSA1, ECGF1 and NGF7S1 was not due to stimulation of the Na+/K+ ATPase since no augmentation of hatching was observed when growth factors were included in media containing ouabain (P < 0.05). The rate of glucose depletion (μg.ml-1.d-1.e-1) by embryos cultured in WM (3.48 +/- 1.04) was greater than in ECGF1 (1.96 +/- 0.62), MSA1 (1.87 +/- 0.55) and NGF7S1 (2.75 +/- 0.37); however, no significant differences were observed, suggesting that the increased hatching in ECGF1, MSA1 and NGF7S1 was not related to enhancement of glucose uptake. The results suggest that FGF1 may be mitogenic for cultured mouse embryos; however, the greater incidence of hatching observed in ECGF1, MSA1 and NGF7S1 seems not to be due to stimulation of the Na+/K+ ATPase or enhancement of glucose uptake.

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Mouse embryo development in media containing growth factors

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