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Endocrine research, v.30 no.1, 2004년, pp.47 - 59
Lewiński, Andrzej (Department of Thyroidology, Medical University of Łó) , Marcinkowska, Magdalena (dź) , Brzeziańska, Ewa (, 5, Dr. Sterling St., 91‐) , Jeziorowska, Anna (425, Łó) , Włoch, Jan (dź) , Brzeziński, Jan (, Poland)
Aim: The aim of this study was to examine the expression of the IGF‐I gene and of genes for IGFBP‐1, ‐2, ‐3, and ‐4 in cells from nodular goiters (NG), and from different human thyroid carcinomas (papillary—PTC, anaplastic—ATC, and medullary—MTC), cultured in monolayers. The influence, exerted by exogenous IGF‐I on the expression of these genes, was also investigated. Methods: Thyroid tissue specimens were obtained from 65 patients during subtotal or total thyroidectomies. After approximately 2–3 weeks of culture, thyroid cells were incubated for 24 hours with IGF‐I in concentrations of: 0, 1, 10 and 100 ng/ml. The total mRNA was isolated according to the method described by Chomczyński and Sacchi with our own modifications. Afterwards, mRNA encoding IGF‐I, IGFBP‐1—IGFBP‐4 and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), were amplified, using the reverse transcription‐polymerase chain reaction (RT‐PCR); GAPDH gene served as a control gene. PCR products were electrophoresed and then submitted to densitometric analysis. Results and conclusions: Our study has shown that in carcinoma cells (ATC, PTC, MTC), IGF‐I reveals a stimulatory influence on the expression of its own gene, that effect being most distinctive in ATC cells. These facts indicate an important role of IGF‐I in the pathogenesis and invasiveness of the analyzed malignant neoplasms.
Le Roith, Derek, Bondy, Carolyn, Yakar, Shoshana, Liu, Jun-Li, Butler, Andrew. The Somatomedin Hypothesis: 2001. Endocrine reviews, vol.22, no.1, 53-74.
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