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ROTATION OF F1-ATPASE: How an ATP-Driven Molecular Machine May Work

Annual review of biophysics and biomolecular structure, v.33, 2004년, pp.245 - 268  

Kinosita, Kazuhiko (1Center for Integrative Bioscience, Okazaki National Research Institutes, Higashiyama 5-1, Myodaiji, Okazaki 444-8585, Japan) ,  Adachi, Kengo (email: kazuhiko@ims.ac.jp) ,  Itoh, Hiroyasu (adachi@ims.ac.jp, 2Tsukuba Research Laboratory, Hamamatsu Photonics KK, Tokodai, Tsukuba 300-2635, Japan, 3CREST "Creation and Application of Soft Nano-Machine, the Hyperfunctional Molecular Machine" Team 13*, Tokodai, Tsukuba 300-2635, Japan)

Abstract

F1-ATPase is a rotary motor made of a single protein molecule. Its rotation is driven by free energy obtained by ATP hydrolysis. In vivo, another motor, Fo, presumably rotates the F1 motor in the reverse direction, reversing also the chemical reaction in F1 to let it synthesize ATP. Here we attempt to answer two related questions, How is free energy obtained by ATP hydrolysis converted to the mechanical work of rotation, and how is mechanical work done on F1 converted to free energy to produce ATP? After summarizing single-molecule observations of F1 rotation, we introduce a toy model and discuss its free-energy diagrams to possibly answer the above questions. We also discuss the efficiency of molecular motors in general.

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