마늘인경에 방사선(⁶⁰Co)을 처리하여 생육특성, 항산화효소인 superoxide dismutase(SOD)와 peroxidase(POD)의 활성, DPPH radical 소거능을 조사하였다. 방사선의 처리선량이 1에서 50Gy로 증가할수록 마늘의 출아속도가 현저히 지연되며, 출아된 인편의 생존율이 현저히 떨어져 10 및 50Gy 처리에서 44.7% 및 0%의 생존율을 보였다. 방사선의 선량이 증가할수록 초장, 추대율이 낮아졌고 구중이 감소하였다. 인편의 superoxide dismutase 활성은 처리 직후에는 5 및 10Gy에서 무처리 보다 증가하나 2세대마늘에서는 차이가 없었다. POD 활성은 10Gy를 처리한 직후는 무처리 보다 1.5배 증가하였으나 2세대인편에서는 오히려 무처리의 46%로 감소하였다. 마늘인편의 DPPH radical 소거능은 무처리가 IC50이 41.6㎎ 인데 비하여 10Gy를 처리한 마늘인편은 21.7㎎로 소거활성이 증가하였다.
Garlic cloves exposed to various dose of gamma radiation (⁶⁰Co) were cultured in the field, and their growth potential, activity of antioxidative enzymes such as superoxide dismutase (SOD) and peroxidase (POD), DPPH radical scavenging capacity were investigated. Emergence of garlic sprouts were retarded by high dose of radiation, and survival rate of the garlic plant were markedly decreased by enhanced dose of radiation. Enzyme activity of SOD and POD at just after treatment were significantly increased by 5 or 10 Gy of radiation. However, SOD activity in the second generation garlic was not affected by the treatment, but POD activity in the second generation which were treated with 10 Gy was decreased to 46% of control. DPPH radical scavenging capacity of garlic clove extract was not changed immediately after radiation treatment, but that in second generations treated with 10 Gy were significantly increased to IC₅₀ 21.7 ㎎ as compared to control, IC₅₀ 41.6 ㎎.
DOI 인용 스타일