초록

아시아틱나리 계통인 신품종 ‘ 홍화’, ‘ 미르’ 두 품종의 기내증식 및 비대를 위해서 고체와 액체배지에서 여러 가지 조건으로 시험한 결과, 생장조절제 처리구에서 ‘ 홍화’ 의 생체중은 BA 1.0㎎ㆍL⁻¹와 IAA 0.1㎎ㆍL⁻¹ 처리구에서 970.6㎎으로 가장 높았고, 자구수는 BA 0.1㎎ㆍL⁻¹와 IAA 0.1㎎ㆍL⁻¹ 혼용처리에서 5.8개로 가장 많았으며, 자구중은 BA 1.0㎎ㆍL⁻¹와 IAA 0.5㎎ㆍL⁻¹혼용처리구에서 301.4㎎으로 가장 높았다. ‘ 미르’ 는 BA 0.1㎎ㆍL⁻¹와 IAA 0.1㎎ㆍL⁻¹ 처리구에서 총 생체중 942.1㎎, 인편당 자구수 4.8개로 가장 높게 나타났고 자구중은 ‘ 홍화’ 와 동일한 BA 1.0㎎ㆍL⁻¹와 IAA 0.5㎎ㆍL⁻¹처리구에서 153.7㎎으로 가장 높았다. ‘ 홍화’ 품종의 생물배양기 액체배양에서는 MS+sucrose 6%+0.1% charcoal 처리구에서 총 생체중 14.7g, 자구중 4.6g, 자구직경 2.1cm로 가장 높게 나타났고 자구중은 대조구의 2배였다. ‘ 미르’ 품종 역시 ½MS+sucrose 6%+0.1% charcoal 인 배지에서 총생체중 13.6g, 자구중 6.0g, 자구직경 2.2cm로 가장 높게 나타나 활성탄 배지가 생육 및 자구비대에 가장 효과적인 것으로 나타났다.

Abstract

For in vitro proliferation and enlargement of bulblet in Lilium asiatic hybrids, ‘Hongwha’ and ‘Mirr’ cultivar were used with solid and bioreactor liquid medium. In the treatment of growth regulators, BA 1.0 ㎎ㆍL⁻¹ and IAA 0.1 ㎎ㆍL⁻¹ concentration was more effective for fresh weight of ‘Hongwha’ as 970.6 mg, the number of bulblets per scale were more at BA 0.1 ㎎ㆍL⁻¹ and IAA 0.1 ㎎ㆍL⁻¹ concentration as 5.8 ea., the fresh weight of bulblet was 301.4 mg at BA 1.0 ㎎ㆍL⁻¹ and IAA 0.5 ㎎ㆍL⁻¹ concentration. In the treatment of ‘Mirr’, the fresh weight was 942.1 mg at BA 0.1 ㎎ㆍL⁻¹ and IAA 0.1 ㎎ㆍL⁻¹ concentration, the numbers of bulblets per scale were more at the same concentration of ‘Hongwha’ as 4.8 ea., the fresh weight of bulblets was higher in the same concentration of ‘Hongwha’ with BA 1.0 ㎎ㆍL⁻¹ and IAA 0.5 ㎎ㆍL⁻¹ as 153.7 mg. In the bioreactor liquid culture of ‘Hongwha’, the MS + 6% sucrose + 0.1% activated charcoal medium was more effective for total fresh weight, fresh weight of bulblets and diameter of bulblet as 14.7 g, 4.6 g and 2.1 cm respectively. And the fresh weight of bulblets was two times compare to control. In the bioreactor liquid culture of ‘Mirr’, the ½MS + 6% sucrose + 0.1% activated charcoal medium was more effective for total fresh weight, fresh weight of bulblet and diameter of bulblet as 13.6 g, 6.0 g and 2.2 cm respectively. Medium added activated charcoal was more effective for growth and enlargement of bulblet.

주제어

#activated charcoal   #ebb &flow   #growth regulators   #‘Hongwha’   #‘Mirr’   #sucrose   #‘홍화’   #‘미르’   #생장조절제   #당   #활성탄  

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