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Abstract ▼ AI-Helper

This study was conducted to establish the system of rapid propagation by flower stalk node culture of Scilla violacea Hutch. We tested the effects of plant growth regulators, salt contents of modified MS medium and sucrose concentration on bulblet formation and expansion. Two distinct pathways, orga...

This study was conducted to establish the system of rapid propagation by flower stalk node culture of Scilla violacea Hutch. We tested the effects of plant growth regulators, salt contents of modified MS medium and sucrose concentration on bulblet formation and expansion. Two distinct pathways, organogenesis through callus and direct bulblet formation, could be recognized in flower stalk node culture of Scilla violacea Hutch. Up to the 100% of bulblet and shoot organogenesis from the flower stalk node attached with pedicel seven days before anthesis was effectively induced by MS medium supplemented with 0.5 ㎎ㆍL⁻¹ NAA and 1.0 ㎎ㆍL⁻¹ BA. Half-strength MS media supplemented with 0.5 ㎎ㆍL⁻¹ NAA and 1.0 ㎎ㆍL⁻¹ BA was more effective than quater-strength or MS medium for induced and maintained a large number of bulblets. Three percent sucrose treatment enhanced bulblet formation and 6 percent treatment sucrose expanded the bulblets. Plantlet regeneration was successfully achieved from bulblets, from flower stalk node with pedicel-derived callus, via shoot bud induction or direct bulblet formation.

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