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Abstract ▼ AI-Helper

Successful application of plant transformation to improve the quality of chrysanthemum will mainly depend on the availability of an efficient regeneration system. In this study, therefore, the capability of regeneration of multiple-somatic embryos (M-SE), the most appropriate as regeneration primord...

Successful application of plant transformation to improve the quality of chrysanthemum will mainly depend on the availability of an efficient regeneration system. In this study, therefore, the capability of regeneration of multiple-somatic embryos (M-SE), the most appropriate as regeneration primordia or explants, was compared to that of other types of explants, and the progress of the 1st somatic embryogenesis from the leaf explant was observed by histological analysis. In leaf tissue, cell divisions in the epidermal layer and subepidermal layers occurred repeatedly and most of these cells became bulky with large vacuoles, but some of them remained small after division and then formed a proembryo. Soon after, the globular stages in somatic embryos were protruded from leaf surface, indicating that the origin of somatic embryo stemmed from the leaf surface. However, there were some embryos formed by organogenesis. Because the cotyledonary stage of somatic embryos contained meristematic tissue, variation or chimera in transgenic somatic embryos could be reduced when agrobacterium was inoculated in the embryos before cotyledonary stage. Finally, the explant of M-SE which showed a high level of regeneration rate compared to leaf and petal explants, formed new proembryos in 10 days after transferred to regeneration medium. Therefore, it was found that M-SE could be very effectively applied as an explant in the construction of plant"s regeneration system.

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