AbstractAtractylenolide III is a major active component in Atractylodes macrocephala. This paper describes a simple, rapid, specific and sensitive method for the quantification of atractylenolide III in rat plasma using a liquid–liquid extraction procedure followed by liquid chromatography mas...
AbstractAtractylenolide III is a major active component in Atractylodes macrocephala. This paper describes a simple, rapid, specific and sensitive method for the quantification of atractylenolide III in rat plasma using a liquid–liquid extraction procedure followed by liquid chromatography mass spectrometric (LC-MS) analysis. A Kromasil 3.5μm C18 column (150mm×2.00mm) was used as the analytical column. Linear detection responses were obtained for atractylenolide III concentration ranging from 5 to 500ngmL−1. The precision and accuracy data, based on intra-day and inter-day variations over 5 days were within 10.29%. The lower limit of quantitation for atractylenolide III was 5ngmL−1, using 0.1mL plasma for extraction and its recoveries were greater than 85% at the low, medium and high concentrations. The method has been successfully applied to a pharmacokinetic study in rats after an oral administration of atractylenolide III with a dose of 20.0mgkg−1. With the lower limits of quantification at 5ngmL−1 for atractylenolide III, this method was proved to be sensitive enough for the pharmacokinetics study of atractylenolide III.
AbstractAtractylenolide III is a major active component in Atractylodes macrocephala. This paper describes a simple, rapid, specific and sensitive method for the quantification of atractylenolide III in rat plasma using a liquid–liquid extraction procedure followed by liquid chromatography mass spectrometric (LC-MS) analysis. A Kromasil 3.5μm C18 column (150mm×2.00mm) was used as the analytical column. Linear detection responses were obtained for atractylenolide III concentration ranging from 5 to 500ngmL−1. The precision and accuracy data, based on intra-day and inter-day variations over 5 days were within 10.29%. The lower limit of quantitation for atractylenolide III was 5ngmL−1, using 0.1mL plasma for extraction and its recoveries were greater than 85% at the low, medium and high concentrations. The method has been successfully applied to a pharmacokinetic study in rats after an oral administration of atractylenolide III with a dose of 20.0mgkg−1. With the lower limits of quantification at 5ngmL−1 for atractylenolide III, this method was proved to be sensitive enough for the pharmacokinetics study of atractylenolide III.
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