$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

Strand-specific, real-time RT-PCR assays for quantification of genomic and positive-sense RNAs of the fish rhabdovirus, Infectious hematopoietic necrosis virus

Journal of virological methods, v.132 no.1/2, 2006년, pp.18 - 24  

Purcell, Maureen K. (Western Fisheries Research Center, 6505 Northeast 65th Street, Seattle, WA 98115, USA) ,  Hart, S. Alexandra (Western Fisheries Research Center, 6505 Northeast 65th Street, Seattle, WA 98115, USA) ,  Kurath, Gael (Western Fisheries Research Center, 6505 Northeast 65th Street, Seattle, WA 98115, USA) ,  Winton, James R. (Western Fisheries Research Center, 6505 Northeast 65th Street, Seattle, WA 98115, USA)

Abstract AI-Helper 아이콘AI-Helper

AbstractThe fish rhabdovirus, Infectious hematopoietic necrosis virus (IHNV), is an important pathogen of salmonids. Cell culture assays have traditionally been used to quantify levels of IHNV in samples; however, real-time or quantitative RT-PCR assays have been proposed as a rapid alternative. For...

주제어

참고문헌 (25)

  1. 2004 Suggested Procedures for the Detection and Identification of Certain Finfish and Shellfish Pathogens 

  2. J. Aquat. Anim. Health Anderson 12 85 2000 10.1577/1548-8667(200006)012<0085:MEREOA>2.0.CO;2 Molecular epidemiology reveals emergence of a virulent infectious hematopoietic necrosis (IHN) virus strain in wild salmon and its transmission to hatchery fish 

  3. J. Virol. Methods Bae 110 185 2003 10.1016/S0166-0934(03)00129-0 Detection of yellow fever virus: a comparison of quantitative real-time PCR and plaque assay 

  4. J. Aquat. Anim. Health Batts 1 284 1989 10.1577/1548-8667(1989)001<0284:EDOIHN>2.3.CO;2 Enhanced detection of infectious hematopoetic necrosis virus and other fish viruses by pretreatment of cell monolayers with polyethylene glycol 

  5. Bootland vol. 3 57 1999 Infectious haematopoietic necrosis virus 

  6. Dis. Aquat. Organ. Corbeil 39 29 1999 10.3354/dao039029 Evaluation of the protective immunogenicity of the N, P, M, NV and G proteins of infectious hematopoietic necrosis virus in rainbow trout Oncorhynchus mykiss using DNA vaccines 

  7. J. Virol. Methods Craggs 94 111 2001 10.1016/S0166-0934(01)00281-6 Development of a strand-specific RT-PCR based assay to detect the replicative forms of hepatitis C virus RNA 

  8. Dis. Aquat. Organ. De la Vega 59 195 2004 10.3354/dao059195 Quantitative real-time RT-PCR demonstrates that handling stress can lead to rapid increases of gill-associated virus (GAV) infection levels in Penaeus monodon 

  9. J. Virol. Drolet 69 2140 1995 10.1128/JVI.69.4.2140-2147.1995 Detection of truncated virus particles in a persistent RNA virus infection in vivo 

  10. Emerson 1119 1985 Virology Rhabdoviruses 

  11. Emerson 245 1987 The Rhabdoviruses Transcription of vesicular stomatitis virus 

  12. J. Fish Dis. Fendrick 5 87 1982 10.1111/j.1365-2761.1982.tb00462.x Comparative sensitivity of five fish cell lines to wild type infectious haematopoietic necrosis virus from two Oregon sources 

  13. Annales de l’Institut Pasteur Virologie Fijan 134E 207 1983 10.1016/S0769-2617(83)80060-4 Some properties of the Epithelioma papulosum cyprini (EPC) cell line from carp Cyprinus carpio 

  14. Dis. Aquat. Organ. Gilad 60 179 2004 10.3354/dao060179 Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR 

  15. J. Virol. Goldberg 77 8812 2003 10.1128/JVI.77.16.8812-8818.2003 Strain variation in an emerging iridovirus of warm-water fishes 

  16. Leong 61 1983 Bacterial and Viral Diseases of Fish. Molecular Studies Synthesis of the structural proteins of infectious hematopoietic necrosis virus 

  17. J. Virol. Methods Lin 100 97 2002 10.1016/S0166-0934(01)00399-8 A novel strand-specific RT-PCR for detection of hepatitis C virus negative-strand RNA (replicative intermediate): evidence of absence or very low level of HCV replication in peripheral blood mononuclear cells 

  18. Dis. Aquat. Organ. Mulcahy 8 29 1990 10.3354/dao008029 Isolation of infectious hematopoietic necrosis virus from a leech (Piscicola salmositica) and a copepod (Salmincola sp.), ectoparasites of sockeye salmon Oncorhynchus nerka 

  19. Virus Res. Nichol 38 159 1995 10.1016/0168-1702(95)00054-T Molecular epizootiology and evolution of the glycoprotein and non-virion protein genes of infectious hematopoietic necrosis virus, a fish rhabdovirus 

  20. J. Fish Dis. Overturf 24 325 2001 10.1046/j.1365-2761.2001.00296.x Real-time PCR for the detection and quantitative analysis of IHNV in salmonids 

  21. J. Virol. Methods Peyrefitte 113 19 2003 10.1016/S0166-0934(03)00218-0 Evidence for in vitro falsely-primed cDNAs that prevent specific detection of virus negative strand RNAs in dengue-infected cells: improvement by tagged RT-PCR 

  22. J. Virol. Methods Pradel 116 103 2004 10.1016/j.jviromet.2003.10.004 Strand specific quantitative real-time PCR to study replication of hepatitis C virus genome 

  23. Rose 129 1987 The Rhabdoviruses Rhabdovirus genomes and their products 

  24. J. Gen. Virol. Shiraki 61 271 1982 10.1099/0022-1317-61-2-271 Virus particles and glycoproteins excreted from cultured cells infected with varicella-zoster virus (VZV) 

  25. J. Virol. Methods Youil 120 23 2004 10.1016/j.jviromet.2004.03.011 Comparative study of influenza virus replication in Vero and MDCK cell lines 

관련 콘텐츠

이 논문과 함께 이용한 콘텐츠

저작권 관리 안내
섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트

맨위로