최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기Acta oto-laryngologica, v.125 no.1, 2005년, pp.72 - 75
Kim, Sun Tae (From the Department of Otolaryngology--Head & Neck Surgery Gachon Medical School Inch'on South Korea) , Choi, Jin Ho (From the Department of Otolaryngology--Head & Neck Surgery Gachon Medical School Inch'on South Korea) , Jeon, Heung Gyu (From the Department of Otolaryngology--Head & Neck Surgery Gachon Medical School Inch'on South Korea) , Cha, Heung Eog (From the Department of Otolaryngology--Head & Neck Surgery Gachon Medical School Inch'on South Korea) , Hwang, Yu Jin (Molecular Biology Laboratory, Gil Medical Center Gachon Medical School Inch'on South Korea) , Chung, Yoo-sam (Department of Otolaryngology, Asan Medical Center Seoul South Korea)
Conclusion PCR using panfungal gene primers is a more sensitive method for fungus detection than fungus culture, both in patients with chronic sinusitis and in normal controls. The presence of fungi alone, however, was insufficient to implicate them as pathogens in chronic sinusitis. Objective Previous findings have suggested that polymerase chain reaction (PCR)-based methods are more sensitive and reliable than conventional culture methods for the detection of fungal DNA. We therefore compared these methods in 82 patients with chronic sinusitis and 40 normal controls. Material and methods The noses of the subjects were irrigated with sterile saline, and the samples collected. The sediment from each irrigation was used for fungus culture and PCR analysis. Results PCR analysis using panfungal gene primers showed that 76/82 (92.5%) patients with chronic sinusitis and 39/40 (97.5%) normal controls were positive. In contrast, fungus cultures were positive in 19/82 (23.2%) patients with chronic sinusitis and 12/40 (30.0%) normal controls. We observed no significant between-group differences in the prevalence of fungus or in the fungal species detected.
Laryngoscope Manning SC 1485 108 1998 10.1097/00005537-199810000-00012
Ferguson BJ. Allergic fungal sinusitis: allergic, infectious, or both? Otolaryngol Head Neck Surg Corey JP 110 113 1995
J Allergy Chin Immunol deShazo RD 24 96 1995 10.1016/S0091-6749(95)70029-3
Schubert, Mark S. Medical treatment of allergic fungal sinusitis. Annals of allergy, asthma, & immunology, vol.85, no.2, 90-98.
Mayo Chin Proc Ponikau JU 877 74 1999 10.4065/74.9.877
Diagnosis of allergic fungal sinusitis. Otolaryngol Head Neck Surg Bent JP 580 111 1994 10.1016/S0194-5998(94)70525-9
Isolation of fungi by standard laboratory methods in patients with chronic rhinosinusitis. Laryngo-scope Lebowitz RA 2189 112 2002
Detection of fungi in the nasal mucosa using polymerase chain reaction. Laryngoscope Catten MD 399 111 2001
Microtitration plate enzyme immunoassay to detect PCR-amplified DNA from Candida species in blood. J Clin Microbiol Fujita SI 962 33 1995
Panfungal PCR and multiplex liquid hybridization for detection of fungi in tissue specimens. J Chin Microbiol Hendolin PH 4186 38 2000
J Chin Microbiol Van Bunk JA 1169 36 1998 10.1128/JCM.36.5.1169-1175.1998
Development of a PCR-based line probe assay for identification of fungal pathogens. J Chin Microbiol Martin C 3735 38 2000
J Chin Microbiol Yamakami Y 2464 34 1996 10.1128/JCM.34.10.2464-2468.1996
General primer-mediated PCR for detection of Aspergillus species. J Chin Microbiol Melchers WJG 1710 32 1994
J Chin Microbiol Jordan JA 2962 32 1994 10.1128/JCM.32.12.2962-2967.1994
a common disorder in Europe? Laryngoscope Braun H 264 113 2003 10.1097/00005537-200302000-00013
*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.