[논문]A microfluidic protease activity assay based on the detection of fluorescence polarization

doi:10.1016/j.aca.2006.06.058

A microfluidic protease activity assay based on the detection of fluorescence polarization

Analytica chimica acta : an international journal devoted to all branches of analytical chemistry, v.577 no.2, 2006년, pp.171 - 177

Kim, Jung Hwan (Life Sciences Division, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Republic of Korea) , Shin, Hyun Joon (Microsystem Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Republic of Korea) , Cho, Hyunju (Life Sciences Division, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Republic of Korea) , Kwak, Seung Min (Microsystem Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Republic of Korea) , Cho, Hansang (Microsystem Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Republic of Korea) , Kim, Tae Song (Microsystem Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Republic of Korea) , Kang, Ji Yoon (Microsystem Research Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Republic of Korea) , Yang, Eun Gyeong (Life Sciences Division, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang, Seoul, Republic of Korea)

Abstract ▼ AI-Helper

AbstractThis article describes a fluorescence polarization (FP)-based protease assay on a microfluidic device that is compatible with fast and reproducible analyses of protease activities. The optical systems were arranged for simultaneously measuring fluorescence intensities of vertical and horizontal polarization planes, and the binding of tetramethylrhodamine (TMR) labeled-biotin with streptavidin was utilized for optimizing FP detection in continuously flowing solutions within 74-μm wide, 12-μm deep microchannels of a glass chip. In developing off-chip FP-based assays for proteinase K, trypsin, papain and elastase, TMR conjugated-casein protein (TMR-α-casein) was employed as a universal substrate. After optimization of the hydrodynamic flow control to allow complete mixing of TMR-α-casein and short proteolysis time as possible, and of buffer composition to minimize protein sticking problems, the developed assay was transferred to the microfluidic chip by monitoring FP changes of TMR-α-casein in the main microchannel. The results indicate that the proposed device would serve as an integrated microfluidic platform with automated injection of reacting species, diffusion-controlled mixing, reaction and detection for protease activities without the need to separate the products.

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참고문헌 (27)

Anal. Chem. Reyes 74 2623 2002 10.1021/ac0202435

상세보기
Nature Atencia 437 648 2005 10.1038/nature04163

상세보기
Electrophoresis Wang 23 713 2002 10.1002/1522-2683(200203)23:5<713::AID-ELPS713>3.0.CO;2-7

상세보기
Lab. Chip Schwarz 1 1 2001 10.1039/b103795c

상세보기
Anal. Bioanal. Chem. Uchiyama 379 375 2004 10.1007/s00216-004-2616-z

상세보기
DDT Eggeling 8 632 2003 10.1016/S1359-6446(03)02752-1

상세보기
Combust. Chem. High Throughput Screen Nikiforov 6 201 2003 10.2174/138620703106298383

상세보기
Curr. Opin. Biotechnol. Pavski 14 65 2003 10.1016/S0958-1669(02)00016-2

상세보기
Electrophoresis Le 23 903 2002 10.1002/1522-2683(200203)23:6<903::AID-ELPS903>3.0.CO;2-2

상세보기
Anal. Chem. Wan 71 4183 1999 10.1021/ac9902796

상세보기
Sens. Actuators B Lippitsch 11 499 1993 10.1016/0925-4005(93)85293-J

상세보기
Analyst Gryczynski 124 1041 1999 10.1039/a900364i

상세보기
Anal. Biochem. McCauley 319 244 2003 10.1016/S0003-2697(03)00297-5

상세보기
Anal. Chem. Potyrailo 70 3419 1998 10.1021/ac9802325

상세보기
Anal. Chim. Acta Yadavalli 507 123 2004 10.1016/j.aca.2003.12.029

상세보기
J. Biol. Chem. Leissring 278 37314 2003 10.1074/jbc.M305627200

상세보기
Anal. Biochem. Homer 191 133 1990 10.1016/0003-2697(90)90399-T

상세보기
BioTechniques Voss 20 286 1996 10.2144/96202rr06

상세보기
Anal. Biochem. Schade 243 1 1996 10.1006/abio.1996.0475

상세보기
Anal. Biochem. Blommel 336 75 2005 10.1016/j.ab.2004.09.023

상세보기
Anal. Chem. Dodge 73 3400 2001 10.1021/ac0015366

상세보기
Anal. Chem. Weiller 74 1702 2002 10.1021/ac011096y

상세보기
Key Eng. Mater. Lee 277 90 2005 10.4028/www.scientific.net/KEM.277-279.90

상세보기
J. Appl. Phys. Beard 89 4667 2001 10.1063/1.1357462

상세보기
Nat. Biotechnol. Hatch 19 461 2001 10.1038/88135

상세보기
Electrophoresis Welder 23 1585 2002 10.1002/1522-2683(200206)23:11<1585::AID-ELPS1585>3.0.CO;2-E

상세보기
Biotechnol. Prog. He 19 544 2003 10.1021/bp0256059

상세보기

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A microfluidic protease activity assay based on the detection of fluorescence polarization

Abstract ▼ AI-Helper

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