Abstract ▼ AI-Helper

A semi-preparative reversed phase liquid chromatography （RPLC） was used to isolate the chemical constituents from the chloroform extract of Patrinia villosa Juss. The separation condition was optimized as follows： the column was YWG C18（10.0 mm × 200ram i.d., 10 μn）, the mobile phase was composed of...

A semi-preparative reversed phase liquid chromatography （RPLC） was used to isolate the chemical constituents from the chloroform extract of Patrinia villosa Juss. The separation condition was optimized as follows： the column was YWG C18（10.0 mm × 200ram i.d., 10 μn）, the mobile phase was composed of CH3CN-H2OHAC （55：45：2, V/V） at a flow rate of 4.0 mL/min, the detection wavelength and sample site were set at 280 nm and 750μL （30 g/L）. Under the optimized conditions, five compounds were isolated by RPLC in one run. Their chemical structures were identified as bolusanthol B （1）; （2S）-5, 7, 2＇, 6＇-tetrahydroxy-6-1avandulylated flavanone （2）; （2S）-5, 2＇, 6＇-trihydroxy-2＂, 2＂-dimethylpyrano [5＂, 6＂： 6, 7] flavanune （3）; orotinin （4） and orotinin-5-methyl ether （5） by spectroscopic methods including ultraviolet, infrared, electrospray mass spectromctry, 1D nuclear magnetic resonance （NMR） and 2D NMR techniques. Among them, compounds 2 and 3 were new compounds, and compounds 1,4 and 5 were isolated from the P. genius for the first time.

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