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Atypical mobilities of single native DNA molecules in microchip electrophoresis revealed by differential interference contrast microscopy

Electrophoresis, v.27 no.21, 2006년, pp.4149 - 4157  

Kang, Seong Ho (Department of Chemistry, Chonbuk National University, Jeonju, South Korea) ,  Lee, Seungah (Department of Chemistry, Chonbuk National University, Jeonju, South Korea) ,  Yeung, Edward S. (Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, IA, USA)

Abstract AI-Helper 아이콘AI-Helper

A transmitted-light optical microscope using differential interference contrast (DIC) was employed to follow the real-time dynamics of different kb-sized single native dsDNA molecules without fluorescent-dye labeling. In a PDMS/glass microchip, the electrophoretic migration velocities of larg...

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참고문헌 (44)

  1. 10.1002/1361-6374(199709)5:3<88::AID-BIO2>3.0.CO;2-2 

  2. 10.1002/1361-6374(199709)5:3<116::AID-BIO5>3.0.CO;2-J 

  3. Yeung, Edward S.. DYNAMICS OF SINGLE BIOMOLECULES IN FREE SOLUTION. Annual review of physical chemistry, vol.55, 97-126.

  4. The Chemical Record Yeung E. S. 123 2001 

  5. Zheng, Jinjian, Yeung, Edward S.. Counting Single DNA Molecules in a Capillary with Radial Focusing. Australian journal of chemistry, vol.56, no.2, 149-.

  6. Zheng, J., Yeung, E. S.. Anomalous Radial Migration of Single DNA Molecules in Capillary Electrophoresis. Analytical chemistry, vol.74, no.17, 4536-4547.

  7. Kang, S. H., Shortreed, M. R., Yeung, E. S.. Real-Time Dynamics of Single-DNA Molecules Undergoing Adsorption and Desorption at Liquid−Solid Interfaces. Analytical chemistry, vol.73, no.6, 1091-1099.

  8. Shortreed, M. R., Li, H., Huang, W.-H., Yeung, E. S.. High-Throughput Single-Molecule DNA Screening Based on Electrophoresis. Analytical chemistry, vol.72, no.13, 2879-2885.

  9. Ma, Y., Shortreed, M. R., Yeung, E. S.. High-Throughput Single-Molecule Spectroscopy in Free Solution. Analytical chemistry, vol.72, no.19, 4640-4645.

  10. Science Xu X. 1106 275 1997 10.1126/science.275.5303.1106 

  11. Xu, Xiao-Hong Nancy, Yeung, Edward S.. Long-Range Electrostatic Trapping of Single-Protein Molecules at a Liquid-Solid Interface. Science, vol.281, no.5383, 1650-1653.

  12. Kang, S. H., Yeung, E. S.. Dynamics of Single-Protein Molecules at a Liquid/Solid Interface: Implications in Capillary Electrophoresis and Chromatography. Analytical chemistry, vol.74, no.24, 6334-6339.

  13. Fan, F.-R. F., Bard, A. J., Guckenberger, R., Heim, M.. STM on Wet Insulators: Electrochemistry or Tunneling?. Science, vol.270, no.5243, 1849-1852.

  14. Funatsu, Takashi, Harada, Yoshie, Tokunaga, Makio, Saito, Kiwamu, Yanagida, Toshio. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution. Nature, vol.374, no.6522, 555-559.

  15. Dovichi, Norman J., Martin, John C., Jett, James H., Trkula, Mitchell., Keller, Richard A.. Laser-induced fluorescence of flowing samples as an approach to single-molecule detection in liquids. Analytical chemistry, vol.56, no.3, 348-354.

  16. Wilkerson Jr., Charles W., Goodwin, Peter M., Ambrose, W. Patrick, Martin, John C., Keller, Richard A.. Detection and lifetime measurement of single molecules in flowing sample streams by laser-induced fluorescence. Applied physics letters, vol.62, no.17, 2030-2032.

  17. Zander, C.. Single-molecule detection in solution: a new tool for analytical chemistry. Fresenius' journal of analytical chemistry, vol.366, no.6, 745-751.

  18. Nie, Shuming., Chiu, Daniel T., Zare, Richard N.. Real-Time Detection of Single Molecules in Solution by Confocal Fluorescence Microscopy. Analytical chemistry, vol.67, no.17, 2849-2857.

  19. Taguchi, Hideki, Ueno, Taro, Tadakuma, Hisashi, Yoshida, Masasuke, Funatsu, Takashi. Single-molecule observation of protein-protein interactions in the chaperonin system. Nature biotechnology, vol.19, no.9, 861-865.

  20. Mollova, Emilia T. Single-molecule fluorescence of nucleic acids. Current opinion in chemical biology, vol.6, no.6, 823-828.

  21. Ishijima, Akihiko, Yanagida, Toshio. Single molecule nanobioscience. Trends in biochemical sciences, vol.26, no.7, 438-444.

  22. Kang, Seong-Ho, Jeong, Seok-Jin, Kim, Dae-Kwang, He, Yan, Yeung, Edward S.. Femtomol Single-DNA Molecules Analysis by Electro Field Strength in a Microfluidic Chip Using TIRFM. Bulletin of the Korean chemical society, vol.26, no.2, 315-318.

  23. Garcia-Parajo, M. F., Segers-Nolten, G. M. J., Veerman, J.-A., Greve, J., van Hulst, N. F.. Real-time light-driven dynamics of the fluorescence emission in single green fluorescent protein molecules. Proceedings of the National Academy of Sciences of the United States of America, vol.97, no.13, 7237-7242.

  24. Haab, B. B., Mathies, R. A.. Single-Molecule Detection of DNA Separations in Microfabricated Capillary Electrophoresis Chips Employing Focused Molecular Streams. Analytical chemistry, vol.71, no.22, 5137-5145.

  25. Kang, S. H., Lee, S., Yeung, E. S.. Direct Observation of Single Native DNA Molecules in a Microchannel by Differential Interference Contrast Microscopy. Analytical chemistry, vol.76, no.15, 4459-4464.

  26. Lee, Seungah, Kim, Youngseong, Kang, Seong Ho. Differential interference contrast microscopy for real-time dynamics and manipulation of single cells in microchannels. Microchemical journal, vol.80, no.2, 107-112.

  27. Biophotonics International Hogan H. 52 2004 

  28. Yeung, E.S., Wang, P., Li, W., Giese, R.W.. Laser fluorescence detector for capillary electrophoresis. Journal of chromatography, vol.608, no.1, 73-77.

  29. Z. wiss. Mikrosk. Allen R. D. 193 69 1969 

  30. 10.1007/978-94-010-1414-4 

  31. Microscopy from the Very Beginning Kapitza H. G. 23 1994 

  32. Guttman, Andras., Cooke, Nelson.. Capillary gel affinity electrophoresis of DNA fragments. Analytical chemistry, vol.63, no.18, 2038-2042.

  33. Ruiz-Martinez, Marie C., Berka, Jan., Belenkii, Alexei., Foret, Frantisek., Miller, Arthur W., Karger, Barry L.. DNA sequencing by capillary electrophoresis with replaceable linear polyacrylamide and laser-induced fluorescence detection. Analytical chemistry, vol.65, no.20, 2851-2858.

  34. Appl. Theor. Electrophor. Bashkin J. 23 6 1996 

  35. Menchen, Steve, Johnson, Ben, Winnik, Mitchell A., Xu, Bai. Flowable networks as DNA sequencing media in capillary columns. Electrophoresis, vol.17, no.9, 1451-1459.

  36. Fung, E. N., Yeung, E. S.. Direct Analysis of Single Rat Peritoneal Mast Cells with Laser Vaporization/Ionization Mass Spectrometry. Analytical chemistry, vol.70, no.15, 3206-3212.

  37. Gao, Q., Yeung, E. S.. A Matrix for DNA Separation: Genotyping and Sequencing Using Poly(vinylpyrrolidone) Solution in Uncoated Capillaries. Analytical chemistry, vol.70, no.7, 1382-1388.

  38. Nonionic Surfactants: Physical Chemistry Becher P. 1967 

  39. Wei, W., Yeung, E. S.. DNA Capillary Electrophoresis in Entangled Dynamic Polymers of Surfactant Molecules. Analytical chemistry, vol.73, no.8, 1776-1783.

  40. Wu, C., Liu, T., Chu, B., Schneider, D. K., Graziano, V.. Characterization of the PEO−PPO−PEO Triblock Copolymer and Its Application as a Separation Medium in Capillary Electrophoresis. Macromolecules, vol.30, no.16, 4574-4583.

  41. Iki, N., Kim, Y., Yeung, E. S.. Electrostatic and Hydrodynamic Separation of DNA Fragments in Capillary Tubes. Analytical chemistry, vol.68, no.24, 4321-4325.

  42. Zheng, J., Yeung, E. S.. Mechanism for the Separation of Large Molecules Based on Radial Migration in Capillary Electrophoresis. Analytical chemistry, vol.75, no.15, 3675-3680.

  43. Zheng, J., Li, H.-W., Yeung, E. S.. Manipulation of Single DNA Molecules via Lateral Focusing in a PDMS/Glass Microchannel. The journal of physical chemistry. B, Condensed matter, materials, surfaces, interfaces & biophysical, vol.108, no.29, 10357-10362.

  44. Grossman, Paul D., Soane, David S.. Orientation effects on the electrophoretic mobility of rod-shaped molecules in free solution. Analytical chemistry, vol.62, no.15, 1592-1596.

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