Objective To study the effects of inhibition and disinfection of Candida albican by the metabolite of Lactobacillus DM9811 in vitro and analysis components of metabolite. Methods To scrape their vaginal secretion and the isolate, culture, identify and preserve 30 strains of Candida albicans from the...
Objective To study the effects of inhibition and disinfection of Candida albican by the metabolite of Lactobacillus DM9811 in vitro and analysis components of metabolite. Methods To scrape their vaginal secretion and the isolate, culture, identify and preserve 30 strains of Candida albicans from the secretion. 1. To dispense sabouraud' s culture liquid named "S", and to dispense sabourud's culture liquid in which the extract I and II of the metabolite of lactobacillus respectively replace distilled water named "S_I" and "SII". The preserved Canduda albicans were 50 μl of the solution of Candida albicans into 5 ml of S_I, all and S, and cultured in incubator at 37 ℃ for 24 hours,and to count the number of bacteria. 2. S] and Sn were diluted doubly every time and determined the minimum inhibitory concentration(MC) where no bacteria were found. 3. To detect volatile acid of extract II of the metabolite of lactobacillus and quantitative analysis by gas chromatography. 4. The detected volatile acid was dispensed to liquide"Y" by the concetration in the extract II. To dispense sabourand's culture liquid "Sy" in which liquide Y replaces distilled water. SII and S_Y were diluted by the concentration of minimum inhibitory concentration plused one named SII * and S_Y*. To inoculate 50 /ul of 10 CFU/ml of Candida albicans into 5 ml of S,Su* and Sy* , cultured in incubator at 37 ℃, and to count the number of bacteria every two hours, total 24 hours amounting to 12 points. To draw the growth curves of Candida albicans in S, Sn * and Sy * . Results l.The number of Candida albicans cultured in S,Si* and Sn for 24 hours were obviously different with nonparametric T-test between Si and S,Sn and S(P<0.01). 2. The minimum inhibitory concentration(MIC) of the extract I was 1:4, the extract U was 1:2.3. The major component of the extract II of the metabolite of lactobacillus was acetic acid detected by gas chromatography, its concentration was 0.235 9 g/100 ml. 4. The growth curves of Candida albicans in S,SII* and S_Y* indicate the the growth of Candida albicans was inhibited by SII* and S_Y*, and the inhibition obvious by SII" than S_Y. Conclusions The metabolite of lactobacillus DM9811 had obvious effects of inhibition and disinfection on Candida albicans and provide a new ecological agents to treat Candida vaginitis.
Objective To study the effects of inhibition and disinfection of Candida albican by the metabolite of Lactobacillus DM9811 in vitro and analysis components of metabolite. Methods To scrape their vaginal secretion and the isolate, culture, identify and preserve 30 strains of Candida albicans from the secretion. 1. To dispense sabouraud' s culture liquid named "S", and to dispense sabourud's culture liquid in which the extract I and II of the metabolite of lactobacillus respectively replace distilled water named "S_I" and "SII". The preserved Canduda albicans were 50 μl of the solution of Candida albicans into 5 ml of S_I, all and S, and cultured in incubator at 37 ℃ for 24 hours,and to count the number of bacteria. 2. S] and Sn were diluted doubly every time and determined the minimum inhibitory concentration(MC) where no bacteria were found. 3. To detect volatile acid of extract II of the metabolite of lactobacillus and quantitative analysis by gas chromatography. 4. The detected volatile acid was dispensed to liquide"Y" by the concetration in the extract II. To dispense sabourand's culture liquid "Sy" in which liquide Y replaces distilled water. SII and S_Y were diluted by the concentration of minimum inhibitory concentration plused one named SII * and S_Y*. To inoculate 50 /ul of 10 CFU/ml of Candida albicans into 5 ml of S,Su* and Sy* , cultured in incubator at 37 ℃, and to count the number of bacteria every two hours, total 24 hours amounting to 12 points. To draw the growth curves of Candida albicans in S, Sn * and Sy * . Results l.The number of Candida albicans cultured in S,Si* and Sn for 24 hours were obviously different with nonparametric T-test between Si and S,Sn and S(P<0.01). 2. The minimum inhibitory concentration(MIC) of the extract I was 1:4, the extract U was 1:2.3. The major component of the extract II of the metabolite of lactobacillus was acetic acid detected by gas chromatography, its concentration was 0.235 9 g/100 ml. 4. The growth curves of Candida albicans in S,SII* and S_Y* indicate the the growth of Candida albicans was inhibited by SII* and S_Y*, and the inhibition obvious by SII" than S_Y. Conclusions The metabolite of lactobacillus DM9811 had obvious effects of inhibition and disinfection on Candida albicans and provide a new ecological agents to treat Candida vaginitis.
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