[논문]Studies in the diabetic mutant mouse: V. Glucose tolerance in mice homozygous and heterozygous for the diabetes (db) gene

Chick, W. L.; Lavine, R. L.; Like, A. A.

doi:10.1007/bf01212235

Studies in the diabetic mutant mouse: V. Glucose tolerance in mice homozygous and heterozygous for the diabetes (db) gene
Etudes de la mutation dbdb chez la souris: V. Tol�rance au glucose dans la souris homozygote et h�t�rozygote pour le g�ne diabetique (db) 원문보기

Diabetologia, v.6 no.3, 1970년, pp.257 - 262

Chick, W. L. , Lavine, R. L. , Like, A. A.

Abstract ▼ AI-Helper

SummaryMice homozygous and heterozygous for the diabetes (db) gene were studied to determine: 1. whether latent carbohydrate intolerance is present in young normoglycemic diabetic mutants (db/db); 2. whether normoglycemic food restricted diabetic mutants are carbohydrate intolerant; and 3. whether mice heterozygous for thedb gene (db/+) manifest abnormalities in glucose tolerance, serum IRI levels or body weights. Blood glucose levels were determined 0, 1/2, 1, 2 and 3 h following intraperitoneal administration of 2 mg glucose/g body weight. Normals (+/+) and diabetics (db/db) showed similar glucose tolerance curves during the first two weeks of life; however, both were markedly glucose intolerant compared to normal adult mice. At 3 weeks a small number of mutants had higher 3 h levels than any achieved in normal mice. By 4 weeks the average value for diabetics prior to glucose loading (0 time) was significantly (P < 0.02) elevated (db/db — 144 mg glucose/100 ml, +/+ = 124 mg glucose/100 ml). Although food restriction reduced blood glucose concentration at 0 time, persistence of carbohydrate intolerance was readily demonstrable following glucose loading. — Abnormalities in heterozygotes (db/+), 3 to 16 months of age, were primarily restricted to male mice, which showed moderate, but statistically significant elevations in blood glucose both at 0 time and following glucose administration. Forty percent of male heterozygotes had higher serum IRI levels than any observed in normal control males. Body weights of male heterozygotes were significantly greater (P<0.01) than those in agematched normals.

Abstract

ZusammenfassungIn den vorliegenden Studien an homozygoten (db/db) und heterozygoten (db +) Mäusen des Diabetes-Stammes wurde untersucht: 1. ob bei den jungen, noch normoglykämischen homozygoten Tieren bereits eine Glucosetoleranz nachzuweisen sei; 2. ob Calorieneinschränkung die Glucosetoleranz homozygoter Tiere beeinflusse; und 3. ob bei heterozygoten Anomalien des Zuckerstoffwechsels nachweisbar seien. Bis zum Alter von zwei Wochen war die Glucosetoleranz homozygot diabetischer Tiere (db/db) derjenigen homozygot normaler Tiere (+/+) vergleichbar. In beiden Fällen war sie aber deutlich schlechter als bei normalen erwachsenen Mäusen. Ab der dritten Woche zeigten einige der künftig diabetischen Tiere gegenüber der Norm erhöhte 3-Stundenwerte. Im Alter von 4 Wochen war bei den diabetischen Tieren der Nullwert signifikant erhöht. Kalorienrestriktion beeinflußte die Glucosetoleranz nicht, obwohl die Blutzuckerkonzentration vor Belastung deutlich niedriger war als bei normal ernährten Tieren. Hétérozygote (db/+) Weibchen (3–16 Monate alt) waren völlig normal, während bei Männchen vor und nach Glucosebelastung signifikant erhöhte Blutzuckerkonzentrationen und, in 40% der Fälle, auch erhöhte Serum-IRI-Konzentrationen gemessen wurden. Das Körpergewicht männlicher Heterozygote war signifikant höher als dasjenige gleichaltriger Normaltiere.

Abstract

RésuméDes souris homozygotes et hétérozygotes porteurs du gène du diabète (db) ont été étudiées dans le but de déterminer: 1. si une intolérance latente aux hydrates de carbone existe chez la jeune sourisdbdb normoglycémique, 2. si la sourisdbdb normoglycémique ayant une alimentation réduite présente une intolérance aux hydrates de carbone, et 3. si la souris hétérozygote pour le gènedb (db/+) montre des anomalies de la tolérance au glucose, des taux d'IRI sériques et du poids corporel. Les taux de glucose sanguin ont été mesurés 0, 30, 60, 120 et 180 min après l'injection intrapéritoneale de 2 mg glucose/ gramme de poids corporel. Les souris normales (+/+) et diabétiques (db/db) ont des courbes de tolérance au glucose semblables au cours des 2 premières semaines de leur vie. Il apparaît pourtant qu'elles manifestent une intolérance marquée au glucose si on les compare avec la souris normale adulte. Un petit nombre de sourisdb/db âgées de 3 semaines présentent des glycémies plus élevées que celles observées chez les souris normales à la 180ème minute du test. A 4 semaines, la glycémie moyenne avant la charge glucosée (0 min) est significativement plus élevée (P< 0.02) chez les animaux diabétiquesdb/db (144 mg glucose %) que chez la souris normale +/+ (124 mg glucose %). Bien que la réduction de nourriture diminue la glycémie à 0 min, l'intolérance au glucose est clairement manifeste après administration du glucose. Chez les animaux hétérozygotes (db/+) âgés de 3 à 16 mois, les anomalies de la tolérance au glucose sont surtout décélables chez les mâles qui montrent une élévation modérée mais statistiquement significative des taux de glucose sanguins à 0 min et après l'administration du glucose. 40% des mâles hétérozygotes ont des taux d'IRI sériques plus élevés que ceux mesurés chez les animaux mâles normaux. Le poids corporel des mâles hétérozygotes est significativement plus élevé (P<0.01) que celui des mâles normaux du même âge.

참고문헌 (17)

Diabetologia W.L. Chick 6 243 1970 10.1007/BF01212233 Chick, W.L., Like, A.A.: Studies in the diabetic mutant mouse. III. Physiological factors associated with alterations in beta cell proliferation. Diabetologia6, 243?251 (1970).

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Diabetologia W.L. Chick 6 252 1970 10.1007/BF01212234 ??: Studies in the diabetic mutant mouse. IV. DBM, a modified diabetic mutant produced by outcrossing of the original strain. Diabetologia6, 252?256 (1970).

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Diabetes C.M. Clark Jr. 17 362 1968 10.2337/diab.17.6.362 Clark, C.M., Jr., Cahill, G.F., Jr., Soeldner, J. S.: Effects of exogenous insulin on the rate of fatty acid synthesis and glucose C-14 utilization in the twenty-day rat fetus. Diabetes17, 362?368 (1968).

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D.L. Coleman 813 1969 Diabetes, Proceedings of the Sixth Congress of the International Diabetes Federation Coleman, D.L., Hummel, K.P.: The mutation, diabetes, in the mouse. In ?Diabetes, Proceedings of the Sixth Congress of the International Diabetes Federation,? Östman, J., Ed., Milner, R.D.G., Co-Ed., pp. 813?820, Amsterdam: Excerpta Medica Foundation 1969.
Diabetologia D.L. Coleman 3 238 1967 10.1007/BF01222201 ? ?: Studies with the mutation, diabetes, in the mouse. Diabetologia3, 238?248 (1967).

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- - Personal communication.
Biochim. biophys. Acta P.R. Davoren 63 150 1962 10.1016/0006-3002(62)90347-5 Davoren, P.R.: The isolation of insulin from a single cat pancreas. Biochim. biophys. Acta63, 150?153 (1962).

상세보기
Diabetologia V.G. Foglia 5 258 1969 10.1007/BF01212094 Foglia, V.G., Basabe, J.C., Chieri, R.A.: Evolution of diabetes after early testosterone treatment in rats. Diabetologia5, 258?259 (1969).

상세보기
Lancet A.St.G. Huggett II 368 1957 10.1016/S0140-6736(57)92595-3 Huggett, A.St.G., Nixon, D.A.: Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose. Lancet1957 II, 368?370.

상세보기
Science K.P. Hummel 153 1127 1966 10.1126/science.153.3740.1127 Hummel, K.P., Dickie, M.M., Coleman, D.L.: Diabetes, a new mutation in the mouse. Science153, 1127?1128 (1966).

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Amer. J. Path. A.A. Like 46 621 1965 Like, A.A., Steinke, J., Jones, E.E., Cahill, G.F., Jr.: Pancreatic studies in mice with spontaneous diabetes mellitus. Amer. J. Path.46, 621?644 (1965).
J. clin. Invest. D.H. Mintz 48 176 1969 10.1172/JCI105966 Mintz, D.H., Chez, R.A., Horger, E.O., III: Fetal insulin and growth hormone metabolism in the subhuman primate. J. clin. Invest.48, 176?186 (1969).

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Diabetes C.R. Morgan 12 115 1963 10.2337/diab.12.2.115 Morgan, C.R., Lazarow, A.: Imimmoassay of insulin: two antibody system. Diabetes12, 115?126 (1963).

상세보기
Diabetologia M. Nakamura 3 212 1967 10.1007/BF01222198 Nakamura, M., Yamada, K.: Studies on a diabetic (KK) strain of the mouse. Diabetologia3, 212?221 (1967).

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Diabetes J.S. Soeldner 14 771 1965 10.2337/diab.14.12.771 Soeldner, J.S., Slone, D.: Critical variables in the radioimmunoassay of serum insulin using the double antibody technique. Diabetes14, 771?779 (1965).

상세보기
Obstet. and Gynec. W.M. Spellacy 30 580 1967 Spellacy, W.M., Gall, S.A., Carlson, K.L.: Carbohydrate metabolism of the normal term newborn: plasma insulin and blood glucose levels during an intravenous glucose tolerance test. Obstet. and Gynec.30, 580?583 (1967).
Endocrinology R.F. Willes 20 520 1969 10.1210/endo-84-3-520 Willes, R.F., Boda, J.M., Manns, J.G.: Insulin secretion by the ovine fetus in utero. Endocrinology20, 520?527 (1969).

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Studies in the diabetic mutant mouse: V. Glucose tolerance in mice homozygous and heterozygous for the diabetes (db) gene
Etudes de la mutation dbdb chez la souris: V. Tol�rance au glucose dans la souris homozygote et h�t�rozygote pour le g�ne diabetique (db) 원문보기

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Studies in the diabetic mutant mouse: V. Glucose tolerance in mice homozygous and heterozygous for the diabetes (db) gene Etudes de la mutation dbdb chez la souris: V. Tol�rance au glucose dans la souris homozygote et h�t�rozygote pour le g�ne diabetique (db) 원문보기

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Studies in the diabetic mutant mouse: V. Glucose tolerance in mice homozygous and heterozygous for the diabetes (db) gene
Etudes de la mutation dbdb chez la souris: V. Tol�rance au glucose dans la souris homozygote et h�t�rozygote pour le g�ne diabetique (db) 원문보기