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Rapid and efficient construction of markerless deletions in the Escherichia coli genome 원문보기

Nucleic acids research, v.36 no.14, 2008년, pp.e84 - e84  

Yu, Byung Jo (Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701 and Biomass Team, Korea Institute of Energy Research, Daejeon, 305-343, Korea) ,  Kang, Kui Hyeon (Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701 and Biomass Team, Korea Institute of Energy Research, Daejeon, 305-343, Korea) ,  Lee, Jun Hyoung (Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701 and Biomass Team, Korea Institute of Energy Research, Daejeon, 305-343, Korea) ,  Sung, Bong Hyun (Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701 and Biomass Team, Korea Institute of Energy Research, Daejeon, 305-343, Korea) ,  Kim, Mi Sun (Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 305-701 and Biomass Team, Korea Institute of Energy Research, Daejeon, 305-343, Korea) ,  Kim, Sun Chang (Department of Biological Sciences, Korea Advanced Institute of Science and Techn)

Abstract AI-Helper 아이콘AI-Helper

We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process ...

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