[논문]Capillary electrophoresis-atmospheric pressure chemical ionization-mass spectrometry using an orthogonal interface: Set-up and system parameters

Hommerson, Paul; Khan, Amjad M.; de Jong, Gerhardus J.; Somsen, Govert W.

doi:10.1021/jasms.8b03512

Capillary electrophoresis-atmospheric pressure chemical ionization-mass spectrometry using an orthogonal interface: Set-up and system parameters

Journal of the American Society for Mass Spectrometry, v.20 no.7, 2009년, pp.1311 - 1318

Hommerson, Paul (Analytical Chemistry, Process R and D , AstraZeneca, Macclesfield , Cheshire , United Kingdom) , Khan, Amjad M. (Department of Biomedical Analysis , Utrecht University , 3508 TB , Utrecht , The Netherlands) , de Jong, Gerhardus J. (Department of Biomedical Analysis , Utrecht University , 3508 TB , Utrecht , The Netherlands) , Somsen, Govert W.

Abstract ▼ AI-Helper

The feasibility of atmospheric pressure chemical ionization (APCI) as an alternative ionization technique for capillary electrophoresis-mass spectrometry (CE-MS) was investigated using a grounded sheath-flow CE-MS sprayer and an orthogonal APCI source. Infusion experiments indicated that highest analyte signals were achieved when the sprayer tip was in close vicinity of the vaporizer entrance. The APCI-MS set-up enabled detection of basic, neutral, and acidic compounds, whereas apolar and ionic compounds could not be detected. In the positive ion mode, analytes could be detected in the entire transfer voltage range (0-5 kV), whereas highest signal intensities were observed when the corona discharge current was between 1000 and 2000 nA. In the negative ion mode, the transfer voltage typically was 500 V and the optimum corona discharge current was 6000 nA. Analyte signals were raised with increasing nebulizing gas pressure, but the pressure was limited to 25 psi to avoid siphoning and current drops. Signal intensities appeared to be optimal and constant over a wide range of sheath liquid flow rate (5-25 µL/min) and vaporizer temperature (200-350 °C). APCI-MS signals were unaffected by the composition of the background electrolyte (BGE), even when it contained sodium phosphate and sodium dodecyl sulfate (SDS). Consequently, BGE composition, sheath-liquid flow rate, and vaporizer temperature can be optimized with respect to the CE separation without affecting the APCI-MS response. The analysis of a mixture of basic compounds and a steroid using volatile and nonvolatile BGEs further demonstrates the feasibility of CE-APCI-MS. Detection limits (S/N = 3) were 1. 6-10 µM injected concentrations.

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참고문헌 (19)

Olivares, Jose A., Nguyen, Nhung T., Yonker, Clement R., Smith, Richard D.. On-line mass spectrometric detection for capillary zone electrophoresis. Analytical chemistry, vol.59, no.8, 1230-1232.

상세보기
Takada, Yasuaki., Sakairi, Minoru., Koizumi, Hideaki.. Atmospheric Pressure Chemical Ionization Interface for Capillary Electrophoresis/Mass Spectrometry. Analytical chemistry, vol.67, no.8, 1474-1476.

상세보기
Muijselaar, Pim G, Otsuka, Koji, Terabe, Shigeru. On-line coupling of partial-filling micellar electrokinetic chromatography with mass spectrometry. Journal of chromatography A, vol.802, no.1, 3-15.

상세보기
10.1002/1522-2683(200109)22:16<3426::AID-ELPS3426>3.0.CO;2-I

상세보기
Tanaka, Yoshihide, Otsuka, Koji, Terabe, Shigeru. Evaluation of an atmospheric pressure chemical ionization interface for capillary electrophoresis–mass spectrometry. Journal of pharmaceutical and biomedical analysis, vol.30, no.6, 1889-1895.

상세보기
Nilsson, S. L., Andersson, C., Sjöberg, P. J. R, Bylund, D., Petersson, P., Jörntén-Karlsson, M., Markides, K. E.. Phosphate buffers in capillary electrophoresis/mass spectrometry using atmospheric pressure photoionization and electrospray ionization. Rapid communications in mass spectrometry : RCM, vol.17, no.20, 2267-2272.

상세보기
Mol, Roelof, de Jong, Gerhardus J., Somsen, Govert W.. On-line capillary electrophoresis-mass spectrometry using dopant-assisted atmospheric pressure photoionization: Setup and system performance. Electrophoresis, vol.26, no.1, 146-154.

상세보기
Hommerson, Paul, Khan, Amjad M., de Jong, Gerhardus J., Somsen, Govert W.. Comparison of atmospheric pressure photoionization and ESI for CZE-MS of drugs. Electrophoresis, vol.28, no.9, 1444-1453.

상세보기
Schappler, Julie, Guillarme, Davy, Prat, Josiane, Veuthey, Jean-Luc, Rudaz, Serge. Coupling CE with atmospheric pressure photoionization MS for pharmaceutical basic compounds: Optimization of operating parameters. Electrophoresis, vol.28, no.17, 3078-3087.

상세보기
Mol, R., de Jong, G. J., Somsen, G. W.. Atmospheric Pressure Photoionization for Enhanced Compatibility in On-Line Micellar Electrokinetic Chromatography−Mass Spectrometry. Analytical chemistry, vol.77, no.16, 5277-5282.

상세보기
Himmelsbach, M., Haunschmidt, M., Buchberger, W., Klampfl, C. W.. Microemulsion Electrokinetic Chromatography with On-Line Atmospheric Pressure Photoionization Mass Spectrometric Detection. Analytical chemistry, vol.79, no.4, 1564-1568.

상세보기
Kolakowski, Beata M., Grossert, J. Stuart, Ramaley, Louis. The importance of both charge exchange and proton transfer in the analysis of polycyclic aromatic compounds using atmospheric pressure chemical ionization mass spectrometry. Journal of the American Society for Mass Spectrometry, vol.15, no.3, 301-310.

상세보기
Perazzolli, Chiara, Mancini, Ines, Guella, Graziano. Benzene-assisted atmospheric-pressure chemical ionization: a new liquid chromatography/mass spectrometry approach to the analysis of selected hydrophobic compounds. Rapid communications in mass spectrometry : RCM, vol.19, no.4, 461-469.

상세보기
Hommerson, P., Khan, A. M., Bristow, T., Niessen, W., de Jong, G. J., Somsen, G. W.. Photon-Independent Gas-Phase-Ion Formation in Capillary Electrophoresis−Mass Spectrometry Using Atmospheric Pressure Photoionization. Analytical chemistry, vol.79, no.14, 5351-5357.

상세보기
Takeda, Sahori, Tanaka, Yoshihide, Yamane, Masataka, Siroma, Zyun, Wakida, Shin-ichi, Otsuka, Koji, Terabe, Shigeru. Ionization of dichlorophenols for their analysis by capillary electrophoresis–mass spectrometry. Journal of chromatography A, vol.924, no.1, 415-420.

상세보기
Hommerson, P., Khan, A.M., de Jong, G.J., Somsen, G.W.. Comparison of electrospray ionization and atmospheric pressure photoionization for coupling of micellar electrokinetic chromatography with ion trap mass spectrometry. Journal of chromatography A, vol.1204, no.2, 197-203.

상세보기
Schappler, Julie, Guillarme, Davy, Rudaz, Serge, Veuthey, Jean-Luc. Microemulsion electrokinetic chromatography hyphenated to atmospheric pressure photoionization mass spectrometry. Electrophoresis, vol.29, no.1, 11-19.

상세보기
Rundlett, K. L., Armstrong, D. W.. Mechanism of Signal Suppression by Anionic Surfactants in Capillary Electrophoresis−Electrospray Ionization Mass Spectrometry. Analytical chemistry, vol.68, no.19, 3493-3497.

상세보기
Somsen, Govert W., Mol, Roelof, de Jong, Gerhardus J.. On-line micellar electrokinetic chromatography–mass spectrometry: feasibility of direct introduction of non-volatile buffer and surfactant into the electrospray interface. Journal of chromatography A, vol.1000, no.1, 953-961.

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Capillary electrophoresis-atmospheric pressure chemical ionization-mass spectrometry using an orthogonal interface: Set-up and system parameters

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