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NTIS 바로가기Biochemical and biophysical research communications, v.390 no.1, 2009년, pp.97 - 102
Yang, J. (n, Hubei Province 430062, PR China) , Xu, J. (Institute of Biochemistry and Molecular Biology, Biology Faculty of Hubei University, Wuhan, Hubei Province 430062, PR China) , Zhong, X. , Zhang, Y. , Wu, Q. , Li, W. , Zhang, X. , Zhen, P. , Yang, X. , Chen, L. , Wang, Y. , Zhu, D. , Zhai, C. , Ma, L.
Short hairpin RNA (shRNA) encoded within an expression vector is an effective tool for exploration of gene function in mammalian cells. Many of the current methods for constructing shRNA expression vectors require cumbersome and time-consuming procedures for identification of the desired recombinant...
Nature Elbashir 411 494 2001 10.1038/35078107 Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Proc. Natl. Acad. Sci. USA Luo 101 5494 2004 10.1073/pnas.0400551101 Small interfering RNA production by enzymatic engineering of DNA (SPEED)
Science Brummelkamp 296 550 2002 10.1126/science.1068999 A system for stable expression of short interfering RNAs in mammalian cells
FEBS Lett. Gou 548 113 2003 10.1016/S0014-5793(03)00630-6 Gene silencing in mammalian cells by PCR-based short hairpin RNA
Proc. Natl. Acad. Sci. USA Sui 99 5515 2002 10.1073/pnas.082117599 A DNA vector-based RNAi technology to suppress gene expression in mammalian cells
Nucleic Acids Res. Gou 32 e134 2004 10.1093/nar/gnh129 Genesilencing in alveolar type II cells using cell-specific promoter in vitro, in vivo
Proc. Natl. Acad. Sci. USA Stegmeier 102 13212 2005 10.1073/pnas.0506306102 A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
BMC Biotechnol. Xu 9 53 2009 10.1186/1472-6750-9-53 Construction of siRNA/miRNA expression vectors based on a one-step PCR process
Nat. Genet. Silva 37 1281 2005 10.1038/ng1650 Second-generation shRNA libraries covering the mouse and human genomes
J. Gene Med. Miyagishi 6 715 2004 10.1002/jgm.556 Optimization of an siRNA-expression system with an improved hairpin and its significant suppressive effects in mammalian cells
Cancer Res. Hayashita 65 9628 2005 10.1158/0008-5472.CAN-05-2352 A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation
Methods Enzymol. Hernandez-Hoyos 392 199 2005 10.1016/S0076-6879(04)92012-5 Analysis of T-cell development by using short interfering RNA to knock down protein expression
Nucleic Acids Res. Zhou 33 e62 2005 10.1093/nar/gni061 An RNA polymerase II construct synthesizes short-hairpin RNA. RNA with a quantitative indicator and mediates highly efficient RNAi
Anal. Biochem. Li 373 2 389 2008 10.1016/j.ab.2007.10.034 Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli
Nat. Genet. Li 37 3 311 2005 10.1038/ng1505 MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules
Biochem. Biophys. Res. Commun. Hung 346 3 707 2006 10.1016/j.bbrc.2006.05.164 A novel siRNA validation system for functional screening and identification of effective RNAi probes in mammalian cells
Nucleic Acids Res. Williams 26 2120 1998 10.1093/nar/26.9.2120 Repressor titration: a novel system for selection and stable maintenance of recombinant plasmids
Gene Hashimoto 207 167 1998 10.1016/S0378-1119(97)00621-5 Construction of a yeast expression system with positive selection for gene insertion in the absence of a specific phenotype
Gene Schneider 197 337 1997 10.1016/S0378-1119(97)00280-1 An epitope tagged mammalian/prokaryotic expression vector with positive selection of cloned inserts
Gene Ahmed 28 37 1984 10.1016/0378-1119(84)90085-4 Plasmid vectors for positive galactose-resistance selection of cloned DNA in Escherichia coli
Gene Bernard 162 159 1995 10.1016/0378-1119(95)00314-V New ccdB-positive-selection cloning vectors with kanamycin or chloramphenicol selectable markers
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