[논문]Equol an isoflavonoid: potential for improved prostate health, in vitro  and in vivo  evidence

Lund, Trent D; Blake, Crystal; Bu, Lihong; Hamaker, Amy N; Lephart, Edwin D

doi:10.1186/1477-7827-9-4

[해외논문] Equol an isoflavonoid: potential for improved prostate health, in vitro and in vivo evidence 원문보기

Reproductive biology and endocrinology : RB&E, v.9, 2011년, pp.4 - 4

Lund, Trent D (Stoelting Co., Wood Dale, IL 60191, USA) , Blake, Crystal (The Department of Physiology and Developmental Biology and the Neuroscience Center, Brigham Young University, Provo, Utah 84602, USA) , Bu, Lihong (MRDDRC Imaging Core, Department of Neurobiology, Children's Hospital Boston and Harvard Medical School, Boston, MA 02115, USA) , Hamaker, Amy N (The Department of Physiology and Developmental Biology and the Neuroscience Center, Brigham Young University, Provo, Utah 84602, USA) , Lephart, Edwin D (The Department of Physiology and Developmental Biology and the Neuroscience Center, Brigham Young University, Provo, Utah 84602, USA)

Abstract ▼ AI-Helper

BackgroundTo determine: in vitro binding affinity of equol for 5alpha-dihydrotestosterone (5alpha-DHT), in vitro effects of equol treatment in human prostate cancer (LNCap) cells, and in vivo effects of equol on rat prostate weight and circulating levels of sex steroid hormones.MethodsFirst, in vitro equol binding affinity for 5alpha-DHT was determined using 14C5alpha-DHT combined with cold 5alpha-DHT (3.0 nM in all samples). These steroids were incubated with increasing concentrations of equol (0-2,000 nM) and analyzed by Sephadex LH-20 column chromatography. 14C5alpha-DHT peak/profiles were determined by scintillation counting of column fractions. Using the 14C5alpha-DHT peak (0 nM equol) as a reference standard, a binding curve was generated by quantifying shifts in the 14C5alpha-DHT peaks as equol concentrations increased. Second, equol's in vitro effects on LNCap cells were determined by culturing cells (48 hours) in the presence of increasing concentrations of dimethyl sulfoxide (DMSO) (vehicle-control), 5alpha-DHT, equol or 5alpha-DHT+equol. Following culture, prostate specific antigen (PSA) levels were quantified via ELISA. Finally, the in vivo effects of equol were tested in sixteen male Long-Evans rats fed a low isoflavone diet. From 190-215 days, animals received 0.1cc s.c. injections of either DMSO-control vehicle (n = 8) or 1.0 mg/kg (body weight) of equol (in DMSO) (n = 8). At 215 days, body and prostate weights were recorded, trunk blood was collected and serum assayed for luteinizing hormone (LH), 5alpha-DHT, testosterone and 17beta-estradiol levels.ResultsMaximum and half maximal equol binding to 5alpha-DHT occurred at approximately 100 nM and 4.8 nM respectively. LNCap cells cultured in the presence of 5alpha-DHT significantly increased PSA levels. However, in the presence of 5alpha-DHT+equol, equol blocked the significant increases in PSA levels from LNCap cells. In vivo equol treatment significantly decreased rat prostate weights and serum 5alpha-DHT levels but did not alter LH, testosterone, and estradiol levels.ConclusionsEquol administration appears to have potential beneficial effects for prostate health and other 5alpha-DHT mediated disorders. Equol administration: reduces PSA levels from LNCap cells under 5alpha-DHT stimulation, decreases rat prostate size, decreases serum 5alpha-DHT levels and androgen hormone action, while not altering other circulating sex steroids or LH levels.

참고문헌 (31)

Manach C Williamson G Morand C Scalbert A Rémésy C Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies Am J Clin Nutr 2005 81 Suppl 230S 242S 15640486
Jamison JR Clinical Guide To Nutrition and Dietary Supplements in Disease 2003 Amsterdam, Netherland, Churchhill Livingstone/Elsevier
Adlercreutz H Phyto-oestrogens and cancer Lancet Oncol 2002 3 364 373 10.1016/S1470-2045(02)00777-5 12107024

상세보기
Setchell KD Brown NM Lydeking-Olsen E The clinical importance of the metabolite equol - a clue to the effectiveness of soy and its isoflavones J Nutr 2002 132 3577 3584 12468591

상세보기
Setchell KD Clerici C Lephart ED Cole SJ Heenan C Castellani D Wolfe BE Nechemias-Zimmer L Brown NM Lund TD Handa RJ Heubi JE S-equol, a potent ligand for estrogen receptor beta, is the exclusive enantiomeric form of the soy isoflavone metabolite produced by human intestinal bacterial flora Am J Clin Nutr 2005 81 1072 1079 15883431
Hounsome N Hounsome B Tomos D Edward-Jones G Changes in Antioxidant Compounds in White Cabbage During Winter Storage Postharvest Biol Tech 2009 52 173 179 10.1016/j.postharvbio.2008.11.004

상세보기
Lund TD Munson DJ Haldy ME Setchell KDR Lephart ED Handa RJ Equol is a novel anti-androgen that inhibits prostate growth and hormone feedback Biol Reprod 2004 70 1188 1195 10.1095/biolreprod.103.023713 14681200

상세보기
Yang Q Fung KM Day WV Kropp BP Lin HK Androgen receptor signaling is required for androgen-sensitive human prostate cancer cell proliferation and survival Cancer Cell International 2005 5 8 10.1186/1475-2867-5-8 15813967

상세보기
Lee C Sutkowski DM Sensibar JA Zelner D Kim I Amsel I Shaw N Prins GS Kozlowski JM Regulation of proliferation and production of prostate-specific antigen in androgen-sensitive prostate cancer cells Endocrinology 1995 136 796 803 10.1210/en.136.2.796 7530653
Ozasa K Nakao M Watanabe Y Hayashi K Miki T Mikami K Mori M Sakauchi F Washio M Ito Y Suzuki K Kubo T Wakai K Tamakoshi A JACC Study Group: Association of serum phytoestrogen concentration and dietary habits in a sample set of the JACC Study J Epidemiol 2005 15 Suppl 2 S196 202 10.2188/jea.15.S196 16127234

상세보기
Hoikkala A Mustonen E Saastamoinen I Jokela T Taponen J Saloniemi H Wähälä K High levels of equol in organic skimmed Finnish cow milk Mol Nutr Food Res 2007 51 782 786 10.1002/mnfr.200600222 17576638

상세보기
Lundh TJO Pettersson HI Martinsson KA Comparative levels of free and conjugated plant estrogens in blood plasma of sheep and cattle fed ostrogenic silage J Agric Food Chem 1990 38 1530 1534 10.1021/jf00097a022

상세보기
Common RH Ainsworth L Identification of equol in the urine of the domestic fowl Biochim Biophys Acta 1961 53 403 404 10.1016/0006-3002(61)90452-8 13880769

상세보기
Kuhn G Hennig U Kalbe C Rehfeldt C Ren MQ Moors S Degen GH Growth performance, carcass characteristics and bioavailability of isoflavones in pigs fed soy bean based diets Arch Anim Nutr 2004 58 265 276 10.1080/00039420412331273295 15570741

상세보기
Lund TD Munson DJ Adlercreutz H Handa RJ Lephart ED Androgen receptor expression in the rat prostate is down-regulated by dietary phytoestrogens Reprod Biol Endocrinol 2004 2 5 10.1186/1477-7827-2-5 14728729

상세보기
Pike J Holmes D Kamalati T Davies D Tolhurst R Mazhar D Fishpool S al-Jehani R Waxman J Zelent A Lemoine NR Ali S Buluwela L Silencing of androgen-regulated genes using a fusion of AR with the PLZF transcriptional repressor Oncogene 2004 23 7561 7570 10.1038/sj.onc.1208030 15334066

상세보기
Legg RL Tolman JR Lovinger CT Lephart ED Setchell KD Christensen MJ Diets high in selenium and isoflavones decrease androgen-regulated gene expression in healthy rat dorsolateral prostate Reprod Biol Endocrinol 2008 6 57 10.1186/1477-7827-6-57 19025659

상세보기
Pendleton JM Tan WW Anai S Chang M Hou W Shiverick KT Rosser CJ Phase II trial of isoflavone in prostate-specific antigen recurrent prostate cancer after previous local therapy BMC Cancer 2008 8 132 10.1186/1471-2407-8-132 18471323

상세보기
Hedlund TE Maroni PD Ferucci PG Dayton R Barnes S Jones K Moore R Ogden LG Wähälä K Sackett HM Gray KJ Long-term dietary habits affect soy isoflavone metabolism and accumulation in prostatic fluid in caucasian men J Nutr 2005 135 1400 1406 15930444

상세보기
Rannikko A Petas A Rannikko S Adlercreutz H Plasma and prostate phytoestrogen concentrations in prostate cancer patients after oral phytoestogen supplementation Prostate 2006 66 82 87 10.1002/pros.20315 16114063

상세보기
Gardner CD Oelrich B Liu JP Feldman D Franke AA Brooks JD Prostatic soy isoflavone concentrations exceed serum levels after dietary supplementation Prostate 2009 69 719 726 10.1002/pros.20922 19180569

상세보기
Ellem SJ Risbriger GP The dual, opposing role of estrogens in the prostate Ann NY Acad Sci 2009 1155 174 186 Steroid Enzymes & Cancer 10.1111/j.1749-6632.2009.04360.x 19250203

상세보기
Lephart ED Porter JP Hedges DW Lund TD Setchell KD Phytoestrogens: implications in neurovascular research Curr Neurovasc Res 2004 1 455 464 10.2174/1567202043361974 16181093
Nomura M Akama KT Alves SE Korach KS Gustafsson JA Pfaff DW Ogawa S Differential distribution of estrogen receptor (ER)-alpha and ER-beta in the midbrain raphe nuclei and periaqueductal gray in male mouse: Predominant role of ER-beta in midbrain serotonergic systems Neuroscience 2005 130 445 456 10.1016/j.neuroscience.2004.09.028 15664701

상세보기
Halbreich U Kahn LS Selective oestrogen receptor modulators-current and future brain and behavior applications Expert Opin Pharmacother 2001 1 1385 1398 10.1517/14656566.1.7.1385

상세보기
Thornton MJ Taylor AH Mulligan K Al-Azzawi F Lyon CC O'Driscoll J Messenger AG The distribution of estrogen receptor beta is distinct to that of estrogen receptor alpha and the androgen receptor in human skin and the pilosebaceous unit J Investig Dermatol Symp Proc 2003 8 100 103 10.1046/j.1523-1747.2003.12181.x 12895004

상세보기
Ashcroft GS Mills SJ Lei K Gibbons L Jeong MJ Taniguchi M Burow M Horan MA Wahl SM Nakayama T Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor J Clin Invest 2003 111 1309 1318 12727922

상세보기
Vij U Kumar A Phyto-oestrogens and prostatic growth Natl Med J India 2004 17 22 26 15115228

상세보기
Akaza H Miyanaga N Takashima N Naito S Hirao Y Tsukamoto T Fujioka T Mori M Kim WJ Song JM Pantuck AJ Comparisons of percent equol producers between prostate cancer patients and controls: case-controlled studies of isoflavones in Japanese, Korean and American residents Jpn J Clin Oncol 2004 34 86 89 10.1093/jjco/hyh015 15067102

상세보기
Magee PJ Raschke M Steiner C Duffin JG Pool-Zobel BL Jokela T Wahala K Rowland IR Equol: a comparison of the effects of the racemic compound with that of the purified S-enantiomer on the growth, invasion, and DNA integrity of breast and prostate cells in vitro Nutr Cancer 2006 54 232 242 10.1207/s15327914nc5402_10 16898868

상세보기
Tanaka M Fujimoto K Chihara Y Torimoto K Yoneda T Tanaka N Hirayama A Miyanaga N Akaza H Hirao Y Isoflavone supplements stimulated the production of serum equol and decreased the serum dihydrotestosterone levels in healthy male volunteers Prostate Cancer Prostatic Dis 2009 12 247 252 10.1038/pcan.2009.10 19597532

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[해외논문] Equol an isoflavonoid: potential for improved prostate health, in vitro and in vivo evidence 원문보기

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