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Abstract ▼ AI-Helper

In Vitro flowering of Cymbidium goerigu was attempted to shorten the breeding peflod by sup-plementing plant growth regulator to the medium, modlfying MS medium composltion or soaking rhizomes into the putative flower mduction solutIon(pulsmg method) Increasing cytokmm level in the agar medium IS re...

In Vitro flowering of Cymbidium goerigu was attempted to shorten the breeding peflod by sup-plementing plant growth regulator to the medium, modlfying MS medium composltion or soaking rhizomes into the putative flower mduction solutIon(pulsmg method) Increasing cytokmm level in the agar medium IS reportedely a mam factor not only to Induce flowenng In Vitro but also make the medIUm brown due to OXI?dation resultmg m delaymg the growth This study was carned out m order to develop a new protocol of flower Induction m Vitro and an alternative method of cytokmm treatment to the rhizome kept from oxidating the agar medium Through this study, NAA must be an essenttal factor m the mduction of plantiets from rhizome The most healthful and strong plantlets were mduced from the rhizome m the MS-9 medium contaming activated charcoal (2 g L-I), NAA (5mg L-1) and banana JUIce (100g L-1) BA also promoted the mduction of flowering m C goermgu Both BA supplement to the medium and BA pulsing positively affected to enhance in vitia flowering of C goermgll As both BA and GA3 was supplemented to the medium or treated to the plantiets, the synergical effect was much more than each alone (98% flowering at most) The dmmeter of pseudo-bulb was an another mam factor to mduce flower m Vitro In thu, expenment. At Ieast 5 mrn diameter of pseudo-bulb was needed to tianslt from vegetative stage to reproductive stage In C. goermgll The alternatIve BA treatment of soaking rhizomes Into the solution for 12 hours before trans-feffing was a successful attempt to prevent medIUm brownmg resulting from oxidation