초록

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Abstract ▼ AI-Helper

This study was carried out to investigate the effects of culture materials, culture media, combination of growth regulators and agar concentrations on plant regeneration of Dionaea muscipula in vitro. Leaf blades, petioles (upper, middle and lower part) and rhizome were subjected to in vitro cultur...

This study was carried out to investigate the effects of culture materials, culture media, combination of growth regulators and agar concentrations on plant regeneration of Dionaea muscipula in vitro. Leaf blades, petioles (upper, middle and lower part) and rhizome were subjected to in vitro culture and the best regeneration was obtained with upper part of petioles. Culturing upper leaf petioles on 1MS, 1/2MS, 1/4MS and modified Parliman medium demonstrated that lower concentration of inorganic nutrients of MS medium was better in the plant regeneration, but best regeneration was obtained on modified Parliman medium. Among the various combinations of growth regulators, the highest number of shoot regeneration (39.8/explant) was observed on modified Parliman medium supplemented with 5μM kinetin and 1μM IAA, and the shoot growth was relatively good. Shoot regeneration was promoted on the medium solidified with 0.6% agar, but growth was better on the lower concentration of agar.