This study was conducted to characterize an isolate of cucumber mosaic virus(CMV-As) from Aster yomena Makino showing mosaic symptoms on the leaves. The virus was multiplied in the leaves of Nicotiana tabacum cv. Xanthi-nc. Physical properties of CMV-As, determined by inoculations on Vigna sinensis ...
This study was conducted to characterize an isolate of cucumber mosaic virus(CMV-As) from Aster yomena Makino showing mosaic symptoms on the leaves. The virus was multiplied in the leaves of Nicotiana tabacum cv. Xanthi-nc. Physical properties of CMV-As, determined by inoculations on Vigna sinensis and the Ouchterlony"s agar gel double diffusion test, were as follows: thermal inactivation point was 65℃, dilution end point was 10⁻³, and longevity in vitro was 3-4 days. The green peach aphid(Myzus persicae was a vector of CMV-As and the percentage of transmissions was about 30%. Ultraviolet absorption pattern of purified CMV-As indicated a maximum at 260㎚, and a minimum at 240㎚. The ratios of Amax Amin, A260 A280, and A280 A260 were 1.27, 1.76, and 0.57, respectively. Based upon the ratio of A260 A280, RNA content of CMV-As was estimated to be 18%. Electron micrograph showed that the purified CMV-As was isometric particle about 30㎚ in diameter with hollow center. Sodium dodecyl sulfate(SDS)-polyacrylamide gel electrophoresis(PAGE) was adopted for determination of the molecular weight and the number of subunit of CMV-As coat protein ; molecular weight was about 25,000 daltons, and one major protein band was shown, which indicated that the virus had one type of subunit. Formamide-agarose gel electrophoresis showed that CMV-As consisted of four genomic RNAs(RNA 1, 2, 3, 4) and a satellite RNA. Estimated molecular weights of RNAs were 1.29, 1.16, 0.74, 0.34, and 0.136×10⁶ daltons, respectively.
This study was conducted to characterize an isolate of cucumber mosaic virus(CMV-As) from Aster yomena Makino showing mosaic symptoms on the leaves. The virus was multiplied in the leaves of Nicotiana tabacum cv. Xanthi-nc. Physical properties of CMV-As, determined by inoculations on Vigna sinensis and the Ouchterlony"s agar gel double diffusion test, were as follows: thermal inactivation point was 65℃, dilution end point was 10⁻³, and longevity in vitro was 3-4 days. The green peach aphid(Myzus persicae was a vector of CMV-As and the percentage of transmissions was about 30%. Ultraviolet absorption pattern of purified CMV-As indicated a maximum at 260㎚, and a minimum at 240㎚. The ratios of Amax Amin, A260 A280, and A280 A260 were 1.27, 1.76, and 0.57, respectively. Based upon the ratio of A260 A280, RNA content of CMV-As was estimated to be 18%. Electron micrograph showed that the purified CMV-As was isometric particle about 30㎚ in diameter with hollow center. Sodium dodecyl sulfate(SDS)-polyacrylamide gel electrophoresis(PAGE) was adopted for determination of the molecular weight and the number of subunit of CMV-As coat protein ; molecular weight was about 25,000 daltons, and one major protein band was shown, which indicated that the virus had one type of subunit. Formamide-agarose gel electrophoresis showed that CMV-As consisted of four genomic RNAs(RNA 1, 2, 3, 4) and a satellite RNA. Estimated molecular weights of RNAs were 1.29, 1.16, 0.74, 0.34, and 0.136×10⁶ daltons, respectively.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.