Abstract Aim To investigate the in vivo antithrombotic and antiplatelet activities and triterpenoid saponin contents of ethanol extract of Acanthopanax sessiliflorus fruits (ASFEtOH). Methods In the present work a quantified ASFEtOH was selected to determine its in vivo antithro...
Abstract Aim To investigate the in vivo antithrombotic and antiplatelet activities and triterpenoid saponin contents of ethanol extract of Acanthopanax sessiliflorus fruits (ASFEtOH). Methods In the present work a quantified ASFEtOH was selected to determine its in vivo antithrombotic activity in a rat arteriovenous shunt thrombosis model and its inhibitory effect on adenosine diphosphate (ADP)-induced platelet aggregation using a platelet aggregometer. Ten triterpenoid saponin contents of the extract of the fruits were determined by a validated ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. Total triterpenoid saponin content was also determined according to the colorimetric method by ultraviolet-visible spectrophotometer. Results After ASFEtOH (125, 250, 500 and 1 000 mg·kg−1/d) was orally administered to rats for 15 d, the in vivo antithrombotic activity on rats was in a concentration-dependant manner showing a significant linear relationship for ASFEtOH (P < 0.05). Meanwhile, ASFEtOH significantly (P < 0.05) inhibited ADP-induced platelet aggregation in vivo on rats. The inhibitions of (16.3 ± 4.7)%, (28.6 ± 4.1)%, (47.9 ± 4.0)% and (61.3 ± 6.6)% were reached for ASFEtOH at doses of 125, 250, 500 and 1 000 mg(kg−1 body weight (bw) respectively, compared to an inhibition of (55.4 ± 7.5)% for the positive control aspirin (27 mg(kg−1/d). The contents of the main triterpenoid saponins 22α-hydroxychiisanoside, momordin Ib and chiisanogenin were (3 755 ± 51.3), (2 461 ± 73.0) and (1 114 ± 75.4) μg·g−1 dry fruit respectively. The amount of total triterpenoid saponins in ASFEtOH was (30.6 ± 0.6) mg of ginsenoside Re equivalents (GRE)/g. Conclusion These experimental results suggest that ASFEtOH has both in vivo antithrombotic and antiplatelet activities.
Abstract Aim To investigate the in vivo antithrombotic and antiplatelet activities and triterpenoid saponin contents of ethanol extract of Acanthopanax sessiliflorus fruits (ASFEtOH). Methods In the present work a quantified ASFEtOH was selected to determine its in vivo antithrombotic activity in a rat arteriovenous shunt thrombosis model and its inhibitory effect on adenosine diphosphate (ADP)-induced platelet aggregation using a platelet aggregometer. Ten triterpenoid saponin contents of the extract of the fruits were determined by a validated ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. Total triterpenoid saponin content was also determined according to the colorimetric method by ultraviolet-visible spectrophotometer. Results After ASFEtOH (125, 250, 500 and 1 000 mg·kg−1/d) was orally administered to rats for 15 d, the in vivo antithrombotic activity on rats was in a concentration-dependant manner showing a significant linear relationship for ASFEtOH (P < 0.05). Meanwhile, ASFEtOH significantly (P < 0.05) inhibited ADP-induced platelet aggregation in vivo on rats. The inhibitions of (16.3 ± 4.7)%, (28.6 ± 4.1)%, (47.9 ± 4.0)% and (61.3 ± 6.6)% were reached for ASFEtOH at doses of 125, 250, 500 and 1 000 mg(kg−1 body weight (bw) respectively, compared to an inhibition of (55.4 ± 7.5)% for the positive control aspirin (27 mg(kg−1/d). The contents of the main triterpenoid saponins 22α-hydroxychiisanoside, momordin Ib and chiisanogenin were (3 755 ± 51.3), (2 461 ± 73.0) and (1 114 ± 75.4) μg·g−1 dry fruit respectively. The amount of total triterpenoid saponins in ASFEtOH was (30.6 ± 0.6) mg of ginsenoside Re equivalents (GRE)/g. Conclusion These experimental results suggest that ASFEtOH has both in vivo antithrombotic and antiplatelet activities.
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