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Fern Phyllitis scolopendrium plants were regenerated from rhizome segments treated with chemical mutagens ethylmethanesulphonate (EMS), nitrosomethylurea (NMU) and colchicine, and we explored optimum condition for each treatment based on regeneration ratio. Optimum concentration were considered to b...

Fern Phyllitis scolopendrium plants were regenerated from rhizome segments treated with chemical mutagens ethylmethanesulphonate (EMS), nitrosomethylurea (NMU) and colchicine, and we explored optimum condition for each treatment based on regeneration ratio. Optimum concentration were considered to be 50 to 100 mM for EMS, and 5 to 10 mM for NMU. EMS and colchicine treatments resulted in only morphological variations, but both chlorophyll and morphological variations were induced when NMU are treated. Three types of M₁M₂ (green, variegated, and yellow) were regenerated from rhizome segments of a variegated plant PscoM10-1. Rhizome segments of these three type M₁M₂ were cultured on media to identify the character separation of M₁V₃ plants regenerated from each types. As a result, two types having green and variegated were regenerated from green plants, and yellow and variegated from yellow plants, respectively. And the three types were regenerated again from variegated type.

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