[논문]Enhancement of differentiation efficiency of hESCs into vascular lineage cells in hypoxia via a paracrine mechanism

Shin, J.M.; Kim, J.; Kim, H.E.; Lee, M.J.; Lee, K.I.; Gyong Yoo, E.; Joo Jeon, Y.; Chae, D.W.K.J.I.; Chung, H.M.

doi:10.1016/j.scr.2011.06.002

Enhancement of differentiation efficiency of hESCs into vascular lineage cells in hypoxia via a paracrine mechanism 원문보기

Stem cell research, v.7 no.3, 2011년, pp.173 - 185

Shin, J.M. (Jung-Il Chae. Department of Dental Pharmacology, School of Dentistry, Brain Korea 21 Project, Chonbuk National University, Jeonju, 561-756, Republic of Korea) , Kim, J. (Jung-Il Chae. Department of Dental Pharmacology, School of Dentistry, Brain Korea 21 Project, Chonbuk National University, Jeonju, 561-756, Republic of Korea) , Kim, H.E. (Department of Dental Pharmacology, School of Dentistry, Brain Korea 21 Project, Chonbuk National University, Jeonju, 561-756, Republic of Korea) , Lee, M.J. , Lee, K.I. , Gyong Yoo, E. , Joo Jeon, Y. , Chae, D.W.K.J.I. , Chung, H.M.

Abstract ▼ AI-Helper

Hypoxia is one way of inducing differentiation due to the activation of the key regulatory factor, Hypoxia-inducible factor 1 alpha (HIF-1α). However, the action of HIF-1α on the differentiation of hESCs was unclear until now. To investigate the effect of hypoxia on the differentiation of hESCs, we compared the differentiation efficacy into vascular lineage cells under normoxic and hypoxic conditions. We observed HIF-1α expression and the related expression of pro-angiogenic factors VEGF, bFGF, Ang-1 and PDGF in hEBs cultured under hypoxic conditions. Along with this, differentiation efficacy into vascular lineage cells was improved under hypoxic conditions. When HIF-1α was blocked by echinomycin, both angiogenic factors and the differentiation efficacy were down-regulated, suggesting that the enhancement of differentiation efficacy was caused by intrinsic up-regulation of HIF-1α and these pro-angiogenic factors under hypoxic condition. This response might be primarily regulated by the HIF-1α signal pathway, and hypoxia might be the key to improving the differentiation of hESCs into vascular lineage cells. Therefore, this study demonstrated that microenvironmental changes (i.e., hypoxia) can improve differentiation efficacy of hESCs into a vascular lineage without exogenous factors via cell-intrinsic up-regulation of angiogenic factors. These facts will contribute to the regulation of stem cell fate.

참고문헌 (43)

Genes Dev. Adelman 13 2478 1999 10.1101/gad.13.19.2478 Multilineage embryonic hematopoiesis requires hypoxic ARNT activity

상세보기
Exp. Biol. Med. (Maywood) Cameron 233 1044 2008 10.3181/0709-RM-263 Activation of hypoxic response in human embryonic stem cell-derived embryoid bodies

상세보기
Diabetes Chen 57 3335 2008 10.2337/db08-0503 Ang-1 gene therapy inhibits hypoxia-inducible factor-1alpha (HIF-1alpha)-prolyl-4-hydroxylase-2, stabilizes HIF-1alpha expression, and normalizes immature vasculature in db/db mice

상세보기
Cardiovasc. Res. Chi 61 437 2004 10.1016/j.cardiores.2003.11.033 Molecular determinants of responses to myocardial ischemia/reperfusion injury: focus on hypoxia-inducible and heat shock factors

상세보기
Circulation Cho 116 2409 2007 10.1161/CIRCULATIONAHA.106.687038 Improvement of postnatal neovascularization by human embryonic stem cell derived endothelial-like cell transplantation in a mouse model of hindlimb ischemia

상세보기
Dev. Cell de Haan 4 241 2003 10.1016/S1534-5807(03)00018-2 In vitro generation of long-term repopulating hematopoietic stem cells by fibroblast growth factor-1

상세보기
Circ. Res. Dell'Era 93 414 2003 10.1161/01.RES.0000089460.12061.E1 Fibroblast growth factor receptor-1 is essential for in vitro cardiomyocyte development

상세보기
Development Fehling 130 4217 2003 10.1242/dev.00589 Tracking mesoderm induction and its specification to the hemangioblast during embryonic stem cell differentiation

상세보기
Lab. Invest. Feraud 81 1669 2001 10.1038/labinvest.3780380 Embryonic stem cell-derived embryoid bodies development in collagen gels recapitulates sprouting angiogenesis

상세보기
Recent Prog. Horm. Res. Ferrara 55 15 2000 Vascular endothelial growth factor and the regulation of angiogenesis

상세보기
Circ. Res. Ferreira 101 286 2007 10.1161/CIRCRESAHA.107.150201 Vascular progenitor cells isolated from human embryonic stem cells give rise to endothelial and smooth muscle like cells and form vascular networks in vivo

상세보기
Stem Cells Goldman 27 1750 2009 10.1002/stem.100 A boost of BMP4 accelerates the commitment of human embryonic stem cells to the endothelial lineage

상세보기
Arthritis Rheum. Hollander 44 1540 2001 10.1002/1529-0131(200107)44:7<1540::AID-ART277>3.0.CO;2-7 Expression of hypoxia-inducible factor 1alpha by macrophages in the rheumatoid synovium: implications for targeting of therapeutic genes to the inflamed joint

상세보기
Ann. N. Y. Acad. Sci. Horowitz 1188 199 2010 10.1111/j.1749-6632.2009.05101.x Heat acclimation-mediated cross-tolerance in cardioprotection: do HSP70 and HIF-1alpha play a role?

상세보기
J. Biol. Chem. Huang 271 32253 1996 10.1074/jbc.271.50.32253 Activation of hypoxia-inducible transcription factor depends primarily upon redox-sensitive stabilization of its alpha subunit

상세보기
10.1038/nbt.1605 James, D., H.S. Nam, M. Seandel, D. Nolan, T. Janovitz, M. Tomishima, L. Studer, G. Lee, D. Lyden, R. Benezra, N. Zaninovic, Z. Rosenwaks, S.Y. Rabbany and S. Rafii. Expansion and maintenance of human embryonic stem cell-derived endothelial cells by TGFbeta inhibition is Id1 dependent. Nat Biotechnol 28:161-166

상세보기
J. Neurooncol. Jensen 78 233 2006 10.1007/s11060-005-9103-z Inhibition of hypoxia inducible factor-1alpha (HIF-1alpha) decreases vascular endothelial growth factor (VEGF) secretion and tumor growth in malignant gliomas

상세보기
J. Biol. Chem. Karakiulakis 282 908 2007 10.1074/jbc.M602178200 Cell type-specific effect of hypoxia and platelet-derived growth factor-BB on extracellular matrix turnover and its consequences for lung remodeling

상세보기
Mol. Pharmacol. Ke 70 1469 2006 10.1124/mol.106.027029 Hypoxia-inducible factor-1 (HIF-1)

상세보기
Stem Cells Dev. Kim 16 269 2007 10.1089/scd.2006.0108 Effective isolation and culture of endothelial cells in embryoid body differentiated from human embryonic stem cells

상세보기
Cancer Res. Kong 65 9047 2005 10.1158/0008-5472.CAN-05-1235 Echinomycin, a small-molecule inhibitor of hypoxia-inducible factor-1 DNA-binding activity

상세보기
Proc. Natl. Acad. Sci. U S A Levenberg 99 4391 2002 10.1073/pnas.032074999 Endothelial cells derived from human embryonic stem cells

상세보기
Blood Levenberg 110 806 2007 10.1182/blood-2006-08-019190 Endothelial potential of human embryonic stem cells

상세보기
10.1038/nprot.2010.31 Levenberg, S., L.S. Ferreira, L. Chen-Konak, T.P. Kraehenbuehl and R. Langer. Isolation, differentiation and characterization of vascular cells derived from human embryonic stem cells. Nat Protoc 5:1115-1126

상세보기
Nucleic Acids Res. Low 14 6785 1986 10.1093/nar/14.17.6785 Echinomycin and distamycin induce rotation of nucleosome core DNA

상세보기
Nucleic Acids Res. May 32 65 2004 10.1093/nar/gkh166 Echinomycin inhibits chromosomal DNA replication and embryonic development in vertebrates

상세보기
Nat Cell. Biol. Ohh 2 423 2000 10.1038/35017054 Ubiquitination of hypoxia-inducible factor requires direct binding to the beta-domain of the von Hippel-Lindau protein

상세보기
Development Pearson 135 1525 2008 10.1242/dev.011767 The stepwise specification of embryonic stem cells to hematopoietic fate is driven by sequential exposure to Bmp4, activin A, bFGF and VEGF

상세보기
Brain Pathol. Plate 4 207 1994 10.1111/j.1750-3639.1994.tb00835.x Molecular mechanisms of developmental and tumor angiogenesis

상세보기
10.1002/stem.295 Prado-Lopez, S., A. Conesa, A. Arminan, M. Martinez-Losa, C. Escobedo-Lucea, C. Gandia, S. Tarazona, D. Melguizo, D. Blesa, D. Montaner, S. Sanz-Gonzalez, P. Sepulveda, S. Gotz, J.E. O'Connor, R. Moreno, J. Dopazo, D.J. Burks and M. Stojkovic. Hypoxia promotes efficient differentiation of human embryonic stem cells to functional endothelium. Stem Cells 28:407-418

상세보기
Placenta Rajakumar 24 199 2003 10.1053/plac.2002.0893 Impaired oxygen-dependent reduction of HIF-1alpha and -2alpha proteins in pre-eclamptic placentae

상세보기
Stem Cells Ramirez-Bergeron 19 279 2001 10.1634/stemcells.19-4-279 Hypoxia-inducible factor and the development of stem cells of the cardiovascular system

상세보기
Development Ramirez-Bergeron 131 4623 2004 10.1242/dev.01310 Hypoxia affects mesoderm and enhances hemangioblast specification during early development

상세보기
Science Thomson 282 1145 1998 10.1126/science.282.5391.1145 Embryonic stem cell lines derived from human blastocysts

상세보기
Cancer Res. Veikkola 60 203 2000 Regulation of angiogenesis via vascular endothelial growth factor receptors

상세보기
FEBS J. Vlaminck 274 5533 2007 10.1111/j.1742-4658.2007.06072.x Dual effect of echinomycin on hypoxia-inducible factor-1 activity under normoxic and hypoxic conditions

상세보기
Proc. Natl. Acad. Sci. U S A Wang 92 5510 1995 10.1073/pnas.92.12.5510 Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension

상세보기
Nat. Biotechnol. Wang 25 317 2007 10.1038/nbt1287 Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo

상세보기
10.1016/j.jocn.2009.03.039 Wu, W., X. Chen, C. Hu, J. Li, Z. Yu and W. Cai. Transplantation of neural stem cells expressing hypoxia-inducible factor-1alpha (HIF-1alpha) improves behavioral recovery in a rat stroke model. J Clin Neurosci 17:92-95

상세보기
FASEB J. Zhang 17 1709 2003 10.1096/fj.02-1111fje Hypoxia induces an autocrine-paracrine survival pathway via platelet-derived growth factor (PDGF)-B/PDGF-beta receptor/phosphatidylinositol 3-kinase/Akt signaling in RN46A neuronal cells

상세보기
10.1111/j.1524-475X.2010.00570.x Zhang, X., L. Liu, X. Wei, Y.S. Tan, L. Tong, R. Chang, M.S. Ghanamah, M. Reinblatt, G.P. Marti, J.W. Harmon and G.L. Semenza. Impaired angiogenesis and mobilization of circulating angiogenic cells in HIF-1alpha heterozygous-null mice after burn wounding. Wound Repair Regen 18:193-201

상세보기
Zhou, Y.F., X.W. Zheng, G.H. Zhang, Z.H. Zong and G.X. Qi. The effect of hypoxia-inducible factor 1-alpha on hypoxia-induced apoptosis in primary neonatal rat ventricular myocytes. Cardiovasc J Afr 21:37-41
Invest Ophthalmol. Vis. Sci. Zhu 48 1735 2007 10.1167/iovs.06-1037 Long-term tolerance to retinal ischemia by repetitive hypoxic preconditioning: role of HIF-1alpha and heme oxygenase-1

상세보기

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Enhancement of differentiation efficiency of hESCs into vascular lineage cells in hypoxia via a paracrine mechanism 원문보기

Abstract ▼ AI-Helper

참고문헌 (43)

이 논문을 인용한 문헌

연구과제 타임라인

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

Enhancement of differentiation efficiency of hESCs into vascular lineage cells in hypoxia via a paracrine mechanism 원문보기

Abstract ▼ AI-Helper

참고문헌 (43)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트