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Measuring plant protein with the Bradford assay : 1. Evaluation and standard method

Journal of chemical ecology, v.15 no.3, 1989년, pp.979 - 992  

Jones, Clive G. ,  Daniel Hare, J. ,  Compton, Steve J.

Abstract AI-Helper 아이콘AI-Helper

The suitability of the Bradford protein assay for measuring plant protein was evaluated and a standard method developed. The assay involves extraction of dried, fresh, or frozen plant material in 0.1 NaOH for 30 min. Replicate 100-μl aliquots of centrifuged supernatant are assayed with 5 ml Bio-Rad ...

참고문헌 (31)

  1. Anal. Biochem. A. Bensadoun 70 241 1976 10.1016/S0003-2697(76)80064-4 Bensadoun, A., andWeinstein, D. 1976. Assay of proteins in the presence of interfering materials.Anal. Biochem. 70:241?250. 

  2. Bio-Rad Laboratories 1985 Publication Bio-Rad Protein Assay Bio-Rad Laboratories 1985. Bio-Rad Protein Assay. Publication 85-0192, Bio-Rad Laboratories, Richmond, California. 

  3. Anal. Biochem. M.M. Bradford 72 248 1976 10.1016/0003-2697(76)90527-3 Bradford, M.M. 1976. A rapid and sensitive method for the estimation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72:248?254. 

  4. Anal. Biochem. S.J. Compton 151 369 1985 10.1016/0003-2697(85)90190-3 Compton, S.J., andJones, G.G. 1985. Mechanism of dye response and interference in the Bradford protein assay.Anal. Biochem. 151:369?374. 

  5. Ecology R.F. Denno 67 116 1986 10.2307/1938509 Denno, R.F., Douglass, L.W., andJacobs, D. 1986. Effects of crowding and host plant nutrition on a wing-dimorphic planthopper.Ecology 67:116?123. 

  6. Biochim. Biophys. Acta R.J. Ellis 463 185 1977 10.1016/0304-4173(77)90008-8 Ellis, R.J. 1977. Protein synthesis by isolated chloroplasts.Biochim. Biophys. Acta 463:185?216. 

  7. J. Bot. J.M.O. Eze 60 1046 1982 Eze, J.M.O., andDumbroff, E.B. 1982. A comparison of the Bradford and Lowry methods for the analysis of protein in chlorophyllous tissue.Can.J. Bot. 60:1046?1049. 

  8. Oecologia S.H. Faeth 68 34 1985 10.1007/BF00379470 Faeth, S.H. 1985. Quantitative defense theory and patterns of feeding by oak insects.Oecologia 68:34?40. 

  9. Oecologia E. Gnaiger 62 289 1984 10.1007/BF00384259 Gnaiger, E., andBitterlich, G. 1984. Proximate biochemical composition and caloric content calculated from elemental CHN analysis: A stoichiometric concept.Oecologia 62:289?298. 

  10. J.B. Harborne 1973 Phytochemical Methods Harborne, J.B. 1973. Phytochemical Methods. Chapman and Hall, London. 

  11. Ecology J.D. Hare 64 345 1983 10.2307/1937081 Hare, J.D. 1983. Seasonal variation in plant-insect associations. Survival ofLeptinotarsa decemlineata onSolatium dulcamara.Ecology 64:345?361. 

  12. Anal. Biochem. G. Krystal 148 451 1985 10.1016/0003-2697(85)90252-0 Krystal, G., Macdonald, C., Munt, B., andAshwell, S. 1985. A method for quantitating nanogram amounts of soluble protein using the principle of silver binding.Anal. Biochem. 148:451?460. 

  13. W.D. Loomis 555 1969 Methods in Enzymology, Vol. 13 Loomis, W.D. 1969. Removal of phenolic compounds during the isolation of plant enzymes, pp. 555?563,in J.M. Lowenstein (ed.). Methods in Enzymology, Vol. 13. Academic Press, New York. 

  14. Phytochemistry W.D. Loomis 5 423 1966 10.1016/S0031-9422(00)82157-3 Loomis, W.D., andBattaile, J. 1966. Plant phenolic compounds and the isolation of plant enzymes.Phytochemistry 5:423?438. 

  15. Phytochemistry W.D. Loomis 18 1049 1979 10.1016/S0031-9422(00)91475-4 Loomis, W.D., Lile, J.D., Sandstrom, R.P., andBurbott, A.J. 1979. Adsorbent polystyrene as an aid in plant enzyme isolation.Phytochemistry 18:1049?1054. 

  16. J. Biol. Chem. O.H. Lowry 193 265 1951 10.1016/S0021-9258(19)52451-6 Lowry, O.H., Rosebrough, N.J., Farr, A.L., andRandall, R.J. 1951. Protein measurement with the Polin phenol reagent.J. Biol. Chem. 193:265?275. 

  17. J. Chem. Ecol. M.M. Martin 11 485 1985 10.1007/BF00989559 Martin, M.M., Rockholm, D.C., andMartin, J.S. 1985. Effects of surfactants, pH, and certain cations of precipitation of proteins by tannins.J. Chem. Ecol. 11:485?494. 

  18. Anal. Biochem. D.L. Marks 147 136 1985 10.1016/0003-2697(85)90019-3 Marks, D.L., Buchsbaum, R., andSwain, T. 1985. Measurement of total protein in plant samples in the presence of tannins.Anal. Biochem. 147:136?143. 

  19. Anal. Biochem. R.L. Mattoo 163 376 1987 10.1016/0003-2697(87)90238-7 Mattoo, R.L., Ishaq, M., andSaleemuddin, M. 1987. Protein assay by Coomassie brilliant blue G-250-binding method is unsuitable for plant tissues rich in phenols and phenolases.Anal. Biochem. 163:376?384. 

  20. Annu. Rev. Ecol. Syst. W.J. Mattson 11 119 1980 10.1146/annurev.es.11.110180.001003 Mattson, W.J. 1980. Herbivory in relation to plant nitrogen content.Annu. Rev. Ecol. Syst. 11:119?161. 

  21. A.B. Maynard 1969 Animal Nutrition Maynard, A.B., andLoosli, J.K. 1969. Animal Nutrition. McGraw-Hill, New York. 

  22. G.L. Peterson 95 1983 Methods in Enzymology, Vol. 91 Peterson, G.L. 1983. Determination of total protein, pp. 95?119,in C.W. Hirs and S. N. Timasheffs (eds.). Methods in Enzymology, Vol. 91. Academic Press, New York. 

  23. Biochem J. W.A. Pierpont 112 609 1969 10.1042/bj1120609 Pierpont, W.A. 1969a. o-Quinones formed in plant extracts: Their reactions with amino acids and peptides.Biochem J. 112:609?616. 

  24. Biochem. J. W.A. Pierpont 112 619 1969 10.1042/bj1120619 Pierpont, W.A. 1969b. o-Quinones formed in plant extracts: Their reaction with bovine serum albumin.Biochem. J. 112:619?627. 

  25. Z. Anal. Chem. E. Riegler 53 242 1914 10.1007/BF01547516 Riegler, E. 1914. Eine metrischer Bestimmungsmethode bei Weisses.Z. Anal. Chem. 53:242?245. 

  26. Annu. Rev. Entomol. J.M. Scriber 26 183 1981 10.1146/annurev.en.26.010181.001151 Scriber, J.M., andSlansky, F., Jr. 1981. The nutritional ecology of immature insects.Annu. Rev. Entomol. 26:183?211. 

  27. F. Slansky Jr. 87 1985 Comprehensive Insect Biochemistry and Physiology, Vol. 4 Slansky, F., Jr., andScriber, J.M. 1985. Food consumption and utilization, pp. 87?163,in G. A. Kerkut and L.I. Gilbert (eds.). Comprehensive Insect Biochemistry and Physiology, Vol. 4. Pergamon Press, Oxford. 

  28. Anal. Biochem. P.K. Smith 150 76 1985 10.1016/0003-2697(85)90442-7 Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J., andKlenk, D.C. 1985. Measurement of protein using bicinchoninic acid.Anal. Biochem. 150:76?85. 

  29. J. Chem. Ecol. J.T. Trumble 13 203 1987 10.1007/BF01020363 Trumble, J.T., Hare, J.D., Musselman, R.C., andMcCool, P.M. 1987. Ozone-induced changes in host-plant suitability: Interactions ofKiefena lycopersicella andLycopersicon esculentum.J. Chem. Ecol. 13:203?217. 

  30. Biochem. Z. O. Warburg 310 384 1941/1942 Warburg, O., andChristian, W. 1941/1942. Isolierung und Kristallisation des garungsferments Enolase.Biochem. Z. 310:384?421. 

  31. Plant Physiol. H.M. Wehr 47 Suppl. 281 1971 Wehr, H.M., andLoomis, W.D. 1971. Amino acid modifications and digestibility in tanned protein.Plant Physiol. 47(Suppl.):281. 

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