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Rapid communications in mass spectrometry : RCM, v.26 no.17, 2012년, pp.1955 - 1964
Liu, Zhirui (School of Pharmacy, Second Military Medical University, Shanghai, 200433, China) , Zhu, Dongliang (School of Pharmacy, Second Military Medical University, Shanghai, 200433, China) , Lv, Lei (Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai, 200438, China) , Li, Yueyue (Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai, 200438, China) , Dong, Xing (School of Pharmacy, Second Military Medical University, Shanghai, 200433, China) , Zhu, Zhenyu (School of Pharmacy, Second Military Medical University, Shanghai, 200433, China) , Chai, Yifeng
RATIONALETimosaponin B‐II (TB‐II) is one of the major bioactive steroid glycosides isolated from Anemarrhena asphodeloides Bge. (Fam. Liliaceae). It has been regarded as a potential lead compound, which may be further developed into a promising new drug for preventing dementia. To fully understand the action mechanism of TB‐II, it is important to study the metabolism profile of this compound in vivo.METHODSHerein, a rapid and sensitive method based on ultrahigh‐performance liquid chromatography (UHPLC)/quadrupole‐time‐of‐flight mass spectrometry (QTOFMS) was established to comprehensively investigate the metabolism of TB‐II in Sprague–Dawley rat urine following oral administration of a single dose of TB‐II at 500.4 mg·kg–1.RESULTSA total of twelve metabolites were detected and identified by means of comparing molecular mass, retention time and spectral pattern of the analytes with those of the parent drug. A possible metabolic pathway on the biotransformation of TB‐II was also investigated and proposed.CONCLUSIONSOxidation, deglycosylation and E‐ring cleavage were found to be the major metabolic processes of the compound in rat. It is the first report on a mammalian metabolism study of timosaponin, a common member of steroid glycosides, in rat urine. Copyright © 2012 John Wiley & Sons, Ltd.
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