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Recombinant tagging system using ribosomal frameshifting to monitor protein expression

Biotechnology and bioengineering, v.110 no.3, 2013년, pp.898 - 904  

Han, Se Jong (Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, Korea) ,  Cho, Sayeon (College of Pharmacy, Chung‐) ,  Lowehhaupt, Ky (Ang University, Seoul, Korea) ,  Park, So‐Young (Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts) ,  Sim, Sang Jun (Department of Chemistry, Sungkyunkwan University, Suwon 440‐) ,  Kim, Yang‐Gyun (746)

Abstract AI-Helper 아이콘AI-Helper

AbstractFor rapid and accurate quantitation of recombinant proteins during expression and after purification, we introduce a new tagging strategy that expresses both target proteins and limitedly tagged target proteins together in a single cell at a constant ratio by utilizing cis‐elements of ...

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