This study performed the semen discrimination test, short tandem repeat (STR) typing, and Y-chromosome specific-STR (Y-STR) typing on five, 30-50-year-old semen stains. All samples reacted positively with the SM test reagent, and we observed sperm heads in all samples microscopically. The quantity o...
This study performed the semen discrimination test, short tandem repeat (STR) typing, and Y-chromosome specific-STR (Y-STR) typing on five, 30-50-year-old semen stains. All samples reacted positively with the SM test reagent, and we observed sperm heads in all samples microscopically. The quantity of DNA extracted from the 43- and 50-year-old samples was much lower than from the other samples. STR typing of the 30-, 32-, 32-, 43-, and 50-year-old semen samples detected a maximum of 13, 15, 15, 11, and 6 of 15 loci, respectively, while Y-STR typing detected 16, 16, 16, 10, and 10 of 16 loci. These results suggest that the semen discrimination test and STR and Y-STR typing can detect extremely old semen stains and are useful for forensic practice.
This study performed the semen discrimination test, short tandem repeat (STR) typing, and Y-chromosome specific-STR (Y-STR) typing on five, 30-50-year-old semen stains. All samples reacted positively with the SM test reagent, and we observed sperm heads in all samples microscopically. The quantity of DNA extracted from the 43- and 50-year-old samples was much lower than from the other samples. STR typing of the 30-, 32-, 32-, 43-, and 50-year-old semen samples detected a maximum of 13, 15, 15, 11, and 6 of 15 loci, respectively, while Y-STR typing detected 16, 16, 16, 10, and 10 of 16 loci. These results suggest that the semen discrimination test and STR and Y-STR typing can detect extremely old semen stains and are useful for forensic practice.
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