[논문]A simple and low-cost device performing blood cell counting based on lens-free shadow imaging technique

Roy, M.; Jin, G.; Seo, D.; Nam, M.H.; Seo, S.

doi:10.1016/j.snb.2014.05.011

A simple and low-cost device performing blood cell counting based on lens-free shadow imaging technique

Sensors and actuators. B, Chemical, v.201, 2014년, pp.321 - 328

Roy, M. , Jin, G. , Seo, D. , Nam, M.H. , Seo, S.

Abstract ▼ AI-Helper

Complete blood count (CBC) is a basic and important diagnostic procedure in pathological laboratories. However, current technologies such as hemocytometry, impedancemetry, or flow-cytometry, are either time-consuming or requiring bulky and high-cost of instrumentation. To address this issue we demonstrate a compact, cost-effective, and automated blood cell counting method based on the lens-free shadow imaging technique. For that a lens-free shadow imaging technique based blood cell counting device was fabricated using a blue light emitting diode (LED) and a complementary metal oxide semiconductor (CMOS) image sensor. The shadow images of the blood cells, i.e., diffraction patterns, were captured by the device and processed automatically using a custom developed algorithm to count and differentiate the cells. The comparative study from 21 outpatient whole blood samples was carried out with the fully automated hematology analyzer (LH 750, Beckman Coulter). The comparison between the lens-free shadow imaging device and the standard reference system showed correlation indices of 0.878 for red blood cell (RBC) and 0.927 for white blood cell (WBC). The linearity comparison gave a statistical trend of y=0.9432x with R2=0.99 for RBC and y=0.7395x with R2=0.92 for WBC. Also the correlation coefficient for WBC subpopulations, i.e., neutrophil, lymphocyte, and monocyte, for each individual sample was >0.90. This cost-effective and compact blood cell counting system can be utilized as a powerful point of care (POC) tool, which could play an important role in primary healthcare, especially in areas with limited resources. Since the system is capable to send the images wirelessly and process them on a moderate smart phone, therefore, the proposed system possesses great potential in telemedicine applications.

주제어

참고문헌 (28)

Clin. Epidemiol. Kramer 46 349 1993 10.1016/0895-4356(93)90149-U Role of the complete blood count in detecting occult focal bacterial infection in the young febrile child

상세보기
Pediatr. Pol. Camitta 87 72 2012 10.1016/S0031-3939(12)70597-8 Optimizing use of the complete blood count

상세보기
Seo 239 2011 CMOS Biomicrosystems: Where Electronics Meet Biology Lensfree imaging cytometry and diagnostics for point-of-care and telemedicine application
Eur. J. Appl. Physiol. Severs 74 234 1996 10.1007/BF00377446 Effects of heat and intermittent exercise on leukocyte and sub-population cell counts

상세보기
J. Am. Stat. Assoc. Berkson 30 414 1935 10.1080/01621459.1935.10502287 Laboratory standards in relation to chance fluctuations of the erythrocyte count as estimated with the hemocytometer

상세보기
J. Zoo Wildl. Med. Dein 25 432 1994 Avian leucocyte counting using the hemocytometer

상세보기
Science Fulwyler 150 910 1965 10.1126/science.150.3698.910 Electronic separation of biological cells by volume

상세보기
Eur. J. Cancer Petersen 15 383 1978 10.1016/0014-2964(79)90072-0 Flow-cytometric demonstration of tumour-cell subpopulations with different DNA content in human colo-rectal carcinoma

상세보기
Cytometry Cheung 65 124 2005 10.1002/cyto.a.20141 Impedance spectroscopy flow cytometry: on-chip label-free cell differentiation

상세보기
Meas. Sci. Technol. Yang 17 2001 2006 10.1088/0957-0233/17/7/045 A cell counting/sorting system incorporated with a microfabricated flow cytometer chip

상세보기
Lab Chip Holmes 9 2881 2009 10.1039/b910053a Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry

상세보기
Lab Chip Shi 13 1257 2013 10.1039/c3lc41059e Four-part leukocyte differential count based on sheathless microflow cytometer and fluorescent dye assay

상세보기
J. Vis. Exp. Mudanyali 34 e1650 2009 Lensless on-chip imaging of cells provides a new tool for high-throughput cell-biology and medical diagnostics
Lab Chip Seo 9 777 2009 10.1039/B813943A Ozcan, lensfree holographic imaging for on-chip cytometry and diagnostics

상세보기
Anal. Chem. Seo 82 4621 2010 10.1021/ac1007915 High-throughput lens-free blood analysis on a chip

상세보기
Sens. Actuators, B: Chem. Kim 157 103 2011 10.1016/j.snb.2011.03.032 LED and CMOS image sensor based hemoglobin concentration measurement technique

상세보기
Biosens. Bioelectron. Jin 38 126 2012 10.1016/j.bios.2012.05.022 Lens-free shadow image based high-throughput continuous cell monitoring technique

상세보기
Sci. Rep. Wei 3 1 2013 10.1038/srep01699 On-chip cytometry using plasmonic nanoparticle enhanced lensfree holography

상세보기
Nat. Photonics Mudanyali 7 247 2013 10.1038/nphoton.2012.337 Wide-field optical detection of nanoparticles using on-chip microscopy and self-assembled nanolenses

상세보기
Nat. Methods Greenbaum 9 889 2012 10.1038/nmeth.2114 Imaging without lenses: achievements and remaining challenges of wide-field on-chip microscopy

상세보기
Int. J. Lab. Hematol. Igout 26 1 2004 10.1111/j.0141-9854.2003.00577.x Evaluation of the coulter LH 750 haematology analyzer compared with flow cytometry as the reference method for WBC, platelet and nucleated RBC count

상세보기
Pediatr. Res. Forestier 20 342 1986 10.1203/00006450-198604000-00017 Hematological values of 163 normal fetuses between 18 and 30 weeks of gestation

상세보기
Clin. Exp. Immunol. Holm 13 29 1973 Haemolytic activity of human blood monocytes lysis of human erythrocytes treated with anti-A serum
Cell. Mol. Bioeng. Seo 1 146 2008 10.1007/s12195-008-0018-6 Multi-color LUCAS: lensfree on-chip cytometry using tunable monochromatic illumination and digital noise reduction

상세보기
Comput. Geosci. Bezdek 10 191 1984 10.1016/0098-3004(84)90020-7 F.C.M.: the fuzzy c-means clustering algorithm

상세보기
Fulekar 2009 Bioinformatics: Applications in Life and Environmental Sciences
Int. J. Nurs. Stud. Bland 47 931 2010 10.1016/j.ijnurstu.2009.10.001 Statistical methods for assessing agreement between two methods of clinical measurement

상세보기
J. Immunol. Castells 138 2184 1987 10.4049/jimmunol.138.7.2184 Evaluation of human peripheral blood leukocytes for mast cell tryptase

상세보기

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

A simple and low-cost device performing blood cell counting based on lens-free shadow imaging technique

Abstract ▼ AI-Helper

주제어

참고문헌 (28)

이 논문을 인용한 문헌

연구과제 타임라인

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

A simple and low-cost device performing blood cell counting based on lens-free shadow imaging technique

Abstract ▼ AI-Helper

주제어

참고문헌 (28)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트