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[해외논문] Efficient production of (R)-(−)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp. nitrilase

Bioprocess and biosystems engineering, v.37 no.7, 2014년, pp.1241 - 1248  

Zhang, Zhi-Jun ,  Pan, Jiang ,  Li, Chun-Xiu ,  Yu, Hui-Lei ,  Zheng, Gao-Wei ,  Ju, Xin ,  Xu, Jian-He

Abstract AI-Helper 아이콘AI-Helper

Recombinant Escherichia coli cells expressing Alcaligenes sp. nitrilase were simply immobilized by direct cross-linking using glutaraldehyde. About 85 % of the total nitrilase activity was recovered under the optimal cross-linking conditions. The thermal stabilities of the cross-linked cells measure...

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