Tae, Hyun Jin
(Department of Veterinary Anatomy, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Korea.)
,
Ahn, Dong Choon
(Department of Veterinary Anatomy, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Korea.)
,
Park, Byung Yong
(Department of Veterinary Anatomy, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Korea.)
,
Sim, Jeong Ha
(Department of Nursing, Jejus University, Jeonju 560-714, Korea.)
,
Kim, In Shik
(Department of Veterinary Anatomy, College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University, Jeonju 561-756, Korea.)
Insulin-like growth (IGF) system은 여러 가지 다양한 심근질환과 연관성이 있는 것으로 알려져 있으나 심근경색에서 IGF system의 변화에 대해서는 밝혀져 있지 않다. 따라서 본 연구에서는 흰쥐 심근경색 모델을 활용하여 IGF system의 변동을 살펴보아 심근경색에서 IGF system의 역할을 규명해보고자 하였다.
60일령의 수컷 흰쥐 왼심장동맥을 결찰하여 심근경색 모델을 만들었고 개흉하여 sham 대조군을 삼았다. 실험군은 심근경색 유발 후 1, 3, 7, 14 및 30일에 희생시켰고 대조군은 가슴안을 열고 30일에 희생시켰다. 혈청과 심근내 IGF-I과 IGF-II 농도와 혈청내 IGF binding protein (IGFBP)-1, IGFBP-3 농도를 측정하였다.
심장동맥을 결찰한 실험군에서 평균 심근경색의 크기는 왼심실의 35.2~42.3%이었다. 혈청내 IGF-I의 농도는 대조군과 비교하여 명확하게 감소하였으나 IGF-II 농도는 차이를 나타내지 않았다. 혈청내 IGFBP-1의 농도는 심근경색 후 1일과 3일에 큰 폭으로 상승하였고 IGFBP-3는 전 실험군에서 유의성 있게 증가하였다. 심근경색 실험동물에서 심근의 IGF-I의 농도는 대조군과 비교하여 명확하게 감소한 반면, IGF-II의 농도는 변화가 없었다.
이상의 결과는 심근경색시 IGF system의 변동이 있음을 확인하였고 심근경색 후 심장의 반응에서 IGF system이 중요한 작용이 있음을 알 수 있었다.
Insulin-like growth (IGF) system은 여러 가지 다양한 심근질환과 연관성이 있는 것으로 알려져 있으나 심근경색에서 IGF system의 변화에 대해서는 밝혀져 있지 않다. 따라서 본 연구에서는 흰쥐 심근경색 모델을 활용하여 IGF system의 변동을 살펴보아 심근경색에서 IGF system의 역할을 규명해보고자 하였다.
60일령의 수컷 흰쥐 왼심장동맥을 결찰하여 심근경색 모델을 만들었고 개흉하여 sham 대조군을 삼았다. 실험군은 심근경색 유발 후 1, 3, 7, 14 및 30일에 희생시켰고 대조군은 가슴안을 열고 30일에 희생시켰다. 혈청과 심근내 IGF-I과 IGF-II 농도와 혈청내 IGF binding protein (IGFBP)-1, IGFBP-3 농도를 측정하였다.
심장동맥을 결찰한 실험군에서 평균 심근경색의 크기는 왼심실의 35.2~42.3%이었다. 혈청내 IGF-I의 농도는 대조군과 비교하여 명확하게 감소하였으나 IGF-II 농도는 차이를 나타내지 않았다. 혈청내 IGFBP-1의 농도는 심근경색 후 1일과 3일에 큰 폭으로 상승하였고 IGFBP-3는 전 실험군에서 유의성 있게 증가하였다. 심근경색 실험동물에서 심근의 IGF-I의 농도는 대조군과 비교하여 명확하게 감소한 반면, IGF-II의 농도는 변화가 없었다.
이상의 결과는 심근경색시 IGF system의 변동이 있음을 확인하였고 심근경색 후 심장의 반응에서 IGF system이 중요한 작용이 있음을 알 수 있었다.
Insulin-like growth factor system (IGF system) has been reported to be associated with the variety of disorders of myocardial function. However, the effect of myocardial infarction (MI) on the IGF system has not been fully described. Thus, the present study was to investigate in more detail the chan...
Insulin-like growth factor system (IGF system) has been reported to be associated with the variety of disorders of myocardial function. However, the effect of myocardial infarction (MI) on the IGF system has not been fully described. Thus, the present study was to investigate in more detail the changes of IGF system in the male rat following myocardial infarction (MI). Ligation of the left coronary artery was performed in male Sprague-Dawley male rats at 60 days of age. Control rats were obtained sham-operated animals. MI rats were sacrificed at 1, 3, 7, 14, and 30 day after ligation of left anterior descending coronary artery. Control rats were sacrificed on 30 day after thoracotomy. Myocardial infarct size was assessed by planimetry and perimetry. Serum and heart concentrations of IGF-I and -II were determined by radioimmunoassay. Serum levels of IGF-binding protein (IGFBP)-1 and IGFBP-3 were analyzed with a twosite immunoradiometric assay. Mean infarct size was 35.2~42.3% of the left ventricle after coronary occlusion in experimental groups. Serum levels of IGF-I were markedly reduced, but the levels of IGF-II were not altered in MI rats compared with shamligated controls. Serum IGFBP-I levels in MI rats were significantly increased at 1 and 3 day compared with sham rats. The levels of serum IGFBP-3 were significantly higher in the ligated rats. IGF-I levels of the infarct/periinfarct area of the left ventricle were significantly decreased in rats with myocardial infarction, whereas the levels of IGF-II remained unchanged. These results demonstrate that the IGF system is altered in the myocardial infarction and suggest that the IGF system plays an important role in the response of the heart to myocardial infarction.
Insulin-like growth factor system (IGF system) has been reported to be associated with the variety of disorders of myocardial function. However, the effect of myocardial infarction (MI) on the IGF system has not been fully described. Thus, the present study was to investigate in more detail the changes of IGF system in the male rat following myocardial infarction (MI). Ligation of the left coronary artery was performed in male Sprague-Dawley male rats at 60 days of age. Control rats were obtained sham-operated animals. MI rats were sacrificed at 1, 3, 7, 14, and 30 day after ligation of left anterior descending coronary artery. Control rats were sacrificed on 30 day after thoracotomy. Myocardial infarct size was assessed by planimetry and perimetry. Serum and heart concentrations of IGF-I and -II were determined by radioimmunoassay. Serum levels of IGF-binding protein (IGFBP)-1 and IGFBP-3 were analyzed with a twosite immunoradiometric assay. Mean infarct size was 35.2~42.3% of the left ventricle after coronary occlusion in experimental groups. Serum levels of IGF-I were markedly reduced, but the levels of IGF-II were not altered in MI rats compared with shamligated controls. Serum IGFBP-I levels in MI rats were significantly increased at 1 and 3 day compared with sham rats. The levels of serum IGFBP-3 were significantly higher in the ligated rats. IGF-I levels of the infarct/periinfarct area of the left ventricle were significantly decreased in rats with myocardial infarction, whereas the levels of IGF-II remained unchanged. These results demonstrate that the IGF system is altered in the myocardial infarction and suggest that the IGF system plays an important role in the response of the heart to myocardial infarction.
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