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To evaluate the effect of German chamomile(Matricaria chamomile L.) extract against xanthine oxidase(XO)/ hypoxanthine(HX) in cultured human skin melanoma cells(SK-MEL-3), the cell adhesion activity and melanin synthesis were measured by colorimetric assay after SK-MEL-3 cells were treated with XO...

To evaluate the effect of German chamomile(Matricaria chamomile L.) extract against xanthine oxidase(XO)/ hypoxanthine(HX) in cultured human skin melanoma cells(SK-MEL-3), the cell adhesion activity and melanin synthesis were measured by colorimetric assay after SK-MEL-3 cells were treated with XO/HX or German chamomile(GC) extract. In this study, XO/HX significantly decreased cell adhesion activity dose-dependently, while GC extract on the XO/HX-induced cytotoxicity significantly increased cell adhesion activity damaged by XO/HX-induced cytotoxicity, and also it showed DPPH-radical scavenging activity. For the measurement of melanin synthesis on GC extract, tyrosinase activity and total amount of melanin was assessed in same cultures. GC extract showed significantly a decresed tyrosinase activity and a total amount of melanin compared with XO/HX-treated group after cultured SK-MEL-3 cells were pretreated with 120㎍ㆍmL⁻¹ or 160㎍ㆍmL⁻¹ GC extract for 2 hours. In light microscopy, GC extract-treated group was more increased than XO/HX-treated group in cell number and cell processes. From these results, it is suggested that XO/HX showed toxic effect on cultured SK-MEL-3 cells, and GC extract was effective in the protection of XO/HX-induced cytotoxicity and melanogenesis in these cultures.

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