[논문]Characterization of long‐term in vitro culture‐related alterations of human tonsil‐derived mesenchymal stem cells: role for CCN1 in replicative senescence‐associated increase in osteogenic differentiation

Yu, Yeonsil; Park, Yoon Shin; Kim, Han Su; Kim, Ha Yeong; Jin, Yoon Mi; Jung, Sung‐; Chul; Ryu, Kyung‐; Ha; Jo, Inho

doi:10.1111/joa.12229

Characterization of long‐term in vitro culture‐related alterations of human tonsil‐derived mesenchymal stem cells: role for CCN1 in replicative senescence‐associated increase in osteogenic differentiation 원문보기

Journal of anatomy, v.225 no.5, 2014년, pp.510 - 518

Yu, Yeonsil (Department of Molecular Medicine, School of Medicine, and Global Top 5 Research Program, Ewha Womans University, Seoul, South Korea) , Park, Yoon Shin (Department of Molecular Medicine, School of Medicine, and Global Top 5 Research Program, Ewha Womans University, Seoul, South Korea) , Kim, Han Su (Department of Otorhinolaryngology‐) , Kim, Ha Yeong (Head & Neck Surgery, School of Medicine, and Global Top 5 Research Program, Ewha Womans University, Seoul, South Korea) , Jin, Yoon Mi (Department of Molecular Medicine, School of Medicine, and Global Top 5 Research Program, Ewha Womans University, Seoul, South Korea) , Jung, Sung‐Chul (Department of Molecular Medicine, School of Medicine, and Global Top 5 Research Program, Ewha Womans University, Seoul, South Korea) , Ryu, Kyung‐Ha (Department of Biochemistry, School of Medicine, and Global Top 5 Research Program, Ewha Womans University, Seoul, South Korea) , Jo, Inho (Department of Pediatrics, School of Medicine, and Global Top 5 Research Program, Ewha Womans University, Seoul, South Korea)

Abstract ▼ AI-Helper

AbstractAlthough mesenchymal stem cells (MSC) isolated from bone marrow and adipose tissues are known to be subjected to in vitro culture‐related alterations in their stem cell properties, such data have not been reported in human tonsil‐derived MSC (T‐MSC). Here, we investigated the culture‐related changes of phenotypes, the senescence, and the differentiation potential of T‐MSC. T‐MSC were serially passaged by a standard protocol, and their characteristics were assessed, including MSC‐specific surface antigen profiles, the senescence, and the differentiation potentials into adipocytes, chondrocytes and osteocytes. Up to at least passage 15, we found no alterations in either MSC‐specific surface marker, CD14, CD34, CD45, CD73 and CD90, or the mRNA expression of embryonic stem cell gene markers, Nanog, Oct4‐A and Sox‐2. However, the expression of CD146, recently identified another MSC marker, dramatically decreased with increasing passages from ~？23% at passage 3 to ~？1% at passage 15. The average doubling time increased significantly from ~？38？h at passage 10 to ~？46？h at passage 15. From passage 10, the cell size increased slightly and SA‐β‐gal staining was evident. Both Alizarin Red S staining and osteocalcin expression showed that the osteogenic differentiation potential increased up to passage 10 and decreased thereafter. However, the adipogenic and chondrogenic differentiation potential decreased passage‐dependently from the start, as evidenced by staining of Oil Red O and Alcian Blue, respectively. Consistent with a passage‐dependent osteogenic differentiation, the expression of CCN1, an angiogenic protein known to be related to both senescence and osteogenesis, also increased up to passage 10. Furthermore, ectopic expression of small interfering RNA against CCN1 at passage 10 significantly reversed Alizarin Red S staining and osteocalcin expression. Altogether, our study demonstrates the characterization of long‐term in vitro cultured T‐MSC and that CCN1 may be involved in mediating a passage‐dependent increase in osteogenic potential of T‐MSC.

주제어

참고문헌 (27)

Bernardo ME , Zaffaroni N , Novara F , et？al. ( 2007 ) Human bone marrow derived mesenchymal stem cells do not undergo transformation after long‐term in vitro culture and do not exhibit telomere maintenance mechanisms . Cancer Res 67 , 9142 – 9149 .

상세보기
Coelho MJ , Fernandes MH ( 2000 ) Human bone cell cultures in biocompatibility testing. Part II: Effect of ascorbic acid, β‐glycerophosphate and dexamethasone on osteoblastic differentiation . Biomaterials 21 , 1095 – 2102 .
Espagnolle N , Guilloton F , Deschaseaux F , et？al. ( 2014 ) CD146 expression on mesenchymal stem cells is associated with their vascular smooth muscle commitment . J Cell Mol Med 18 , 104 – 114 .

상세보기
Go MJ , Takenaka C , Ohgushi H ( 2008 ) Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells maintains their expansion and differentiation capabilities . Exp Cell Res 314 , 1147 – 1154 .

상세보기
Halfon S , Abramov N , Grinblat B , et？al. ( 2011 ) Markers distinguishing mesenchymal stem cells from fibroblasts are downregulated with passaging . Stem Cells Dev 20 , 53 – 66 .
Hayflick L ( 1965 ) The limited in vitro lifetime of human diploid cell strains . Exp Cell Res 37 , 614 – 636 .

상세보기
Janjanin S , Djouad F , Shanti RM , et？al. ( 2008 ) Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells . Arthritis Res Ther 10 , 1186 .
Kassem M ( 2004 ) Mesenchymal stem cells: biological characteristics and potential clinical applications . Cloning Stem Cells 6 , 369 – 374 .

상세보기
Lee SY , Lim J , Khang G , et？al. ( 2009 ) Enhanced ex vivo expansion of human adipose tissue‐derived mesenchymal stromal cells by fibroblast growth factor‐2 and dexamethasone . Tissue Eng 15 , 2491 – 2499 .
Liu TM , Wu YN , Guo XM , et？al. ( 2008 ) Effects of ectopic Nanog and Oct4 overexpression on mesenchymal stem cells . Stem Cells Dev 18 , 1013 – 1022 .
Madeira A , Silva CL , Santos F , et？al. ( 2012 ) Human mesenchymal stem cell expression program upon extended ex‐vivo culture, as revealed by 2‐DE‐based quantitative proteomics . PLoS ONE 7 , e43523 .
Mandana MB , Kamran A , Fatemeh T , et？al. ( 2006 ) Aging of mesenchymal stem cell in vitro . BMC Cell Biol 7 , 14 .

상세보기
Mueller SM , Glowacki J ( 2001 ) Age‐related decline in the osteogenic potential of human bone marrow cells cultured in three‐dimensional collagen sponges . J Cell Biochem 82 , 583 – 590 .

상세보기
Nishida S , Endo N , Yamagiwa H , et？al. ( 1999 ) Number of osteoprogenitor cells in human bone marrow markedly decreases after skeletal maturation . J Bone Miner Metab 17 , 171 – 177 .

상세보기
Quan T , Qin Z , Voorhees JJ , et？al. ( 2012 ) Cysteine‐rich protein 61 (CCN1) mediates replicative senescence‐associated aberrant collagen homeostasis in human skin fibroblasts . J Cell Biochem 113 , 3011 – 3018 .

상세보기
Rosa AL , Beloti MM ( 2005 ) Development of the osteoblast phenotype of serial cell subcultures from human bone marrow . Braz Dent J 16 , 225 – 230 .

상세보기
Ryu KH , Cho KA , Park HS , et？al. ( 2012 ) Tonsil‐derived mesenchymal stromal cells: evaluation of biologic, immunologic and genetic factors for successful banking . Cytotherapy 14 , 1193 – 1202 .

상세보기
Si W , Kang Q , Luu HH , et？al. ( 2006 ) CCN1/Cyr61 is regulated by the canonical Wnt signal and plays an important role in Wnt3A‐induced osteoblast differentiation of mesenchymal stem cells . Mol Cell Biol 26 , 2955 – 2964 .

상세보기
Stenderup K , Justuesen J , Clausen C , et？al. ( 2003 ) Aging is associated with decreased maximal life span and accelerated senescence of bone marrow stromal cells . Bone 33 , 919 – 926 .

상세보기
Strioga M , Viswanathan S , Darinskas A , et？al. ( 2012 ) Same or not the same? Comparison of adipose tissue‐derived versus bone marrow‐derived mesenchymal stem and stromal cells . Stem Cells Dev 21 , 2724 – 2752 .
Su JL , Chiou J , Tang CH , et？al. ( 2009 ) CYR61 regulates BMP‐2‐dependent osteoblast differentiation through the α v β 3 integrin/integrin‐linked kinase/ERK pathway . J Biol Chem 285 , 31325 – 31336 .
Sugiura F , Kitoh H , Ishiguro N ( 2004 ) Osteogenic potential of rat mesenchymal stem cells after several passages . Biochim Biophys Res Commun 316 , 233 – 239 .
Svinareva DA , Shipunova IN , Ol'shanskaia IuV , et？al. ( 2010 ) The basic properties of mesenchymal stromal cells from the donor bone marrow: superficial markers . Ter Arkh 82 , 52 – 56 .

상세보기
Ter Brugge PJ , Jansen JA ( 2002 ) In vitro osteogenic differentiation of rat bone marrow cells subcultured with and without dexamethasone . Tissue Eng 8 , 321 – 331 .

상세보기
Vellasamy S , Sandrasaigaran P , Vidyadaran S , et？al. ( 2012 ) Isolation and characterisation of mesenchymal stem cells derived from human placenta tissue . World J Stem Cells 4 , 53 – 61 .

상세보기
Wagner W , Bork S , Horn P , et？al. ( 2009 ) Aging and replicative senescence have related effects on human stem and progenitor cells . PLoS ONE 4 , e5846 .
Wolfgang W , Patrick H , Mirco C , et？al. ( 2008 ) Replicative senescence of mesenchymal stem cells: a continuous and organized process . PLoS ONE 3 , e2213 .

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Characterization of long‐term in vitro culture‐related alterations of human tonsil‐derived mesenchymal stem cells: role for CCN1 in replicative senescence‐associated increase in osteogenic differentiation 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (27)

이 논문을 인용한 문헌

연구과제 타임라인

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

Characterization of long‐term in vitro culture‐related alterations of human tonsil‐derived mesenchymal stem cells: role for CCN1 in replicative senescence‐associated increase in osteogenic differentiation 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (27)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트