[논문]Glucose biosensor based on GOx/HRP bienzyme at liquid-crystal/aqueous interface

Khan, M.; Park, S.Y.

doi:10.1016/j.jcis.2015.07.010

[해외논문] Glucose biosensor based on GOx/HRP bienzyme at liquid-crystal/aqueous interface

Journal of colloid and interface science, v.457, 2015년, pp.281 - 288

Khan, M. , Park, S.Y.

Abstract ▼ AI-Helper

Glucose oxidase (GOx) and horseradish peroxidase (HRP) were co-immobilized to the polyacrylicacid block of a poly(acrylicacid-b-4-cyanobiphenyl-4'-undecylacrylate) (PAA-b-LCP) copolymer in water. PAA-b-LCP was strongly anchored by the LCP block in 4-cyano-4'-pentylbiphenyl (5CB) which was contained in a transmission electron microscope (TEM) grid for glucose detection. The optimal conditions for the performance of the TEM grid glucose biosensor were studied in terms of the activity and stability of the immobilized enzymes. Glucose in water was detected by the 5CB changing from a planar to a homeotropic orientation, as observed through a polarized optical microscope. The TEM biosensor detected glucose concentrations at ≥0.02mM, with an optimal GOx/HRP molar ratio of 3/1. This glucose biosensor has characteristics of enzyme sensitivity and stability, reusability, the ease and selective glucose detection which may provide a new way of detecting glucose.

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참고문헌 (29)

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[해외논문] Glucose biosensor based on GOx/HRP bienzyme at liquid-crystal/aqueous interface

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[해외논문] Glucose biosensor based on GOx/HRP bienzyme at liquid-crystal/aqueous interface

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참고문헌 (29)

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