[논문]Efficient production of free fatty acids from soybean meal carbohydrates

Wang, Dan; Thakker, Chandresh; Liu, Ping; Bennett, George N.; San, Ka‐; Yiu

doi:10.1002/bit.25633

Efficient production of free fatty acids from soybean meal carbohydrates

Biotechnology and bioengineering, v.112 no.11, 2015년, pp.2324 - 2333

Wang, Dan (Department of Bioengineering, Rice University, 6100 Main Street, MS‐) , Thakker, Chandresh (362, Houston, Texas, 77005‐) , Liu, Ping (1892) , Bennett, George N. (Department of BioSciences, Rice University, Houston, Texas) , San, Ka‐Yiu (Department of Bioengineering, Rice University, 6100 Main Street, MS‐)

Abstract ▼ AI-Helper

ABSTRACTConversion of biomass feedstock to chemicals and fuels has attracted increasing attention recently. Soybean meal, containing significant quantities of carbohydrates, is an inexpensive renewable feedstock. Glucose, galactose, and fructose can be obtained by enzymatic hydrolysis of soluble carbohydrates of soybean meal. Free fatty acids (FFAs) are valuable molecules that can be used as precursors for the production of fuels and other value‐added chemicals. In this study, free fatty acids were produced by mutant Escherichia coli strains with plasmid pXZ18Z (carrying acyl‐ACP thioesterase (TE) and (3R)‐hydroxyacyl‐ACP dehydratase) using individual sugars, sugar mixtures, and enzymatic hydrolyzed soybean meal extract. For individual sugar fermentations, strain ML211 (MG1655 fadD‐ fabR‐)/pXZ18Z showed the best performance, which produced 4.22, 3.79, 3.49 g/L free fatty acids on glucose, fructose, and galactose, respectively. While the strain ML211/pXZ18Z performed the best with individual sugars, however, for sugar mixture fermentation, the triple mutant strain XZK211 (MG1655 fadD‐ fabR‐ ptsG‐)/pXZ18Z with an additional deletion of ptsG encoding the glucose‐specific transporter, functioned the best due to relieved catabolite repression. This strain produced approximately 3.18 g/L of fatty acids with a yield of 0.22 g fatty acids/g total sugar. Maximum free fatty acids production of 2.78 g/L with a high yield of 0.21 g/g was achieved using soybean meal extract hydrolysate. The results suggested that soybean meal carbohydrates after enzymatic treatment could serve as an inexpensive feedstock for the efficient production of free fatty acids. Biotechnol. Bioeng. 2015;112: ???–???. © 2015 Wiley Periodicals, Inc.

주제어

참고문헌 (46)

Andersson C , Hodge D , Berglund KA , Rova U . 2007 . Effect of different carbon sources on the production of succinic acid using metabolically engineered Escherichia coli . Biotechnol Progr 23 : 381 – 388 .
Balzer G , Thakker C , Bennett GN , San KY . 2013 . Metabolic engineering Escherichia coli to minimize byproduct formate and improving succinate productivity through NADH availability by heterologous expression of NAD(+)‐dependent formate dehydrogenase . Metab Eng 20 : 1 – 8 .
Cao Y , Yang J , Xian M , Xu X , Liu W . 2010 . Increasing unsaturated fatty acid contents in Escherichia coli by coexpression of three different genes . Appl Microbiol Biotechnol 87 : 271 – 280 .

상세보기
Cho H , Cronan J , Jr. 1995 . Defective export of a periplasmic enzyme disrupts regulation of fatty acid synthesis . J Biol Chem 270 : 4216 – 4219 .

상세보기
Datsenko KA , Wanner BL . 2000 . One‐step inactivation of chromosomal genes in Escherichia coli K‐12 using PCR products . Proc Nat Acad Sci 97 : 6640 – 6645 .
Dellomonaco C , Clomburg JM , Miller EN , Gonzalez R . 2011 . Engineering reversal of the β‐oxidation cycle for the synthesis of fuels and chemicals . Nature 476 : 355 – 359 .
Dien BS , Nichols NN , Bothast RJ . 2002 . Fermentation of sugar mixtures using Escherichia coli catabolite repression mutants engineered for production of L‐lactate acid . J Ind Microbiol Biotechnol 29 : 221 – 227 .
Eldridge AC , Black LT , Wolf JW . 1979 . Carbohydrate composition of soybean flours, protein concentrates, and isolates . J Agric Food Chem 27 : 799 – 802 .
Feng Y , Cronan JE . 2011 . Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters . Mol Microbiol 80 : 195 – 218 .

상세보기
Fridjonssson O , Watzlawick H , Gehweiler A , Rohrhirsch T , Mattes R . 1999 . Cloning of the gene encoding a novel thermostable galactosidase from Thermus brockianus I TI360 . Appl Environ Microbiol 65 : 3955 – 3963 .
Giannoccaro E , Wang YJ , Chen P . 2006 . Effects of solvent, temperature, time, solvent‐to‐sample ratio, sample size, and defatting on the extraction of soluble sugars in soybean . J Food Sci 71 : C59 – C64 .
Janßen HJ , Steinbüchel A . 2014 . Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels . Biotechnol Biofuels 7 : 7 – 33 .

상세보기
Jiang P , Cronan J , Jr. 1994 . Inhibition of fatty acid synthesis in Escherichia coli in the absence of phospholipid synthesis and release of inhibition by thioesterase action . J Bacteriol 176 : 2814 – 2821 .

상세보기
Jönsson L , Alriksson B , Nilvebrant N . 2013 . Bioconversion of lignocelluloses: Inhibitors and detoxification . Biotechnol Biofuels 6 : 16 – 25 .
Karr‐Lilienthal LK , Grieshop CM , Spears JK , Fahey GC . 2005 . Amino acid, carbohydrate, and fat composition of soybean meals prepared at 55 commercial U.S. soybean processing plants . J Agric Food Chem 53 : 2146 – 2150 .

상세보기
Kim JH , Block DE , Mills DA . 2010 . Simultaneous consumption of pentose and hexose sugars: An optimal microbial phenotype for efficient fermentation of lignocellulosic biomass . Appl Microbiol Biotechnol 88 : 1077 – 1085 .

상세보기
Kim KR , Oh DK . 2013 . Production of hydroxyl fatty acids by microbial fatty acid‐hydroxylation enzymes . Biotechnol Adv 31 : 1473 – 1485 .
Kim NJ , Li H , Jung K , Chang HN , Lee PC . 2011 . Ethanol production from algal hydrolysates using Escherichia coli KO11 . Bioresour Technol 102 : 7466 – 7469 .
Lennen R , Braden G , West R , Dumesic J , Pfleger B . 2010 . A process for microbial hydrocarbon synthesis: Overproduction of fatty acids in Escherichia coli and catalytic conversion to alkanes . Biotechnol Bioeng 106 : 193 – 202 .

상세보기
Li M , Zhang X , Agrawal A , San K . 2012 . Effect of acetate formation pathway and long chain fatty acid CoA‐ligase on the free fatty acid production in E. coli expressing acyl‐ACP thioesterase from Ricinus communis . Metab Eng 14 : 380 – 387 .
Lin H , Bennett GN , San K . 2005 . Metabolic engineering of aerobic succinate production systems in Escherichia coli to improve process productivity and achieve the maximum theoretical succinate yield . Metab Eng 7 : 116 – 127 .

상세보기
Liu K . 1997 . Soybeans: Chemistry, technology, and utilization . New York : Chapman and Hall .
Liu T , Vora H , Khosla C . 2010 . Quantitative analysis and engineering of fatty acid biosynthesis in E. coli . Metab Eng 12 : 378 – 386 .

상세보기
Lu X , Vora H , Khosla C . 2008 . Overexpression of free fatty acids in E. coli : Implications for biodiesel production . Metab Eng 10 : 333 – 339 .
Mazumdar S , Bang J , Oh MK . 2013 . L ‐Lactate production from seaweed hydrolysate of Laminaria japonica using metabolically engineered Escherichia coli . Appl Biochem Biotechnol 172 : 1938 – 1952 .
McCue L , Thompson W , Carmack C , Ryan MP , Liu JS , Derbyshire V , Lawrence CE . 2001 . Phylogenetic footprinting of transcription factor binding sites in proteobacterial genomes . Nucleic Acids Res 29 : 774 – 782 .

상세보기
Nickolau B , Perera M , Brachova L , Shanks B . 2008 . Platform chemicals for a biorenewable chemical industry . Plant J 54 : 536 – 545 .
O'Fallon JV , Busboom JR , Nelson ML , Gaskins CT . 2007 . A direct method for fatty acid methyl ester synthesis: Application to wet meat tissues, oils, and feedstuffs . J Anim Sci 85 : 1511 – 1521 .

상세보기
Ooi S , Pee H . 1985 . Processing for industrial fatty acids‐II . J Am Oil Chem Soc 62 : 348 – 351 .

상세보기
Peralta‐Yahya PB , Zhang F , del Cardayre SB , Keasling JD . 2012 . Microbial engineering for the production of advanced biofuels . Nature 488 : 320 – 328 .

상세보기
San KY , Li M . 2013 . Bacteria and method for synthesizing fatty acids . Vol. WO/2013/059218, USA.
Sánchez AM , Bennett GN , San K . 2005 . Novel pathway engineering design of the anaerobic central metabolic pathway in Escherichia coli to increase succinate yield and productivity . Metab Eng 7 : 229 – 239 .

상세보기
Schmid K , Schupfner M , Schmitt R . 1982 . Plasmid‐mediated uptake and metabolism of sucrose by Escherichia coli K‐12 . J Bacteriol 151 : 68 – 76 .
Schmid K , Schmitt R . 1976 . Raffinose metabolism in Escherichia coli K12. Purification and properties of a new a‐galactosidase specified by a transmissible plasmid . Eur. J Biochem 67 : 95 – 104 .
Shimizu K . 2013 . Metabolic regulation of a bacterial cell system with emphasis on Escherichia coli metabolism . ISRN Biochem 645983 : 1 – 47 .
Stephanopoulos G . 2007 . Challenges in engineering microbes for biofuels production . Science 315 : 801 – 804 .

상세보기
Thakker C , San K , Bennett GN . 2013 . Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions . Bioresour Technol 130 : 398 – 405 .

상세보기
Voelker T , Davies H . 1994 . Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of plant medium‐chain acyl‐acyl carrier protein thioesterase . J Bacteriol 176 : 7320 – 7327 .
Wang D , Li Q , Yang M , Zhang Y , Su Z , Xing J . 2011 . Efficient production of succinic acid from corn stalk hydrolysates by a recombinant Escherichia coli with ptsG mutation . Process Biochem 46 : 365 – 371 .

상세보기
Wu H , San KY . 2014 . Efficient odd straight medium chain free fatty acid production by metabolically engineered Escherichia coli . Biotechnol Bioeng 9999 : 1 – 11 .
Wu H , Lee J , Karanjikar M , San KY . 2014a . Efficient free fatty acid production from woody biomass hydrolysate using metabolically engineered Escherichia coli . Bioresour Technol 169 : 119 – 125 .

상세보기
Wu H , Karanjikar M , San KY . 2014b . Metabolic engineering of Escherichia coli for efficient free fatty acid production from glycerol . Metab Eng 25 : 82 – 91 .

상세보기
Zha Y , Muilwijk B , Coulier L , Punt PJ . 2012 . Inhibitory compounds in lignocellulosic biomass hydrolysates during hydrolysate fermentation process . J Bioprocess Biotechniq 2 : 1 – 11 .
Zhang XJ , Li M , Agrawal A , San KY . 2011 . Efficient free fatty acid production in Escherichia coli using plant acyl‐ACP thioesterase . Metab Eng 13 : 713 – 722 .
Zhang YM , Marrakchi H , Rock CO . 2002 . The FabR (YijC) transcription factor regulates unsaturated fatty acid biosynthesis in Escherichia coli . J Biol Chem 277 : 15558 – 15565 .

상세보기
Zhang YM , Rock CO . 2009 . Transcriptional regulation in bacterial membrane lipid synthesis . J Lipid Res 50 : S115 – S119 .

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Efficient production of free fatty acids from soybean meal carbohydrates

Abstract ▼ AI-Helper

주제어

참고문헌 (46)

이 논문을 인용한 문헌

관련 콘텐츠

원문 URL 링크

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트