[논문]Comparative analysis of gut microbiota in elderly people of urbanized towns and longevity villages

Park, Se-Hoon; Kim, Kyung-Ah; Ahn, Young-Tae; Jeong, Jin-Ju; Huh, Chul-Sung; Kim, Dong-Hyun

doi:10.1186/s12866-015-0386-8

Comparative analysis of gut microbiota in elderly people of urbanized towns and longevity villages 원문보기

BMC microbiology, v.15, 2015년, pp.49 -

Park, Se-Hoon (R &B D Center, Korea Yakult Co., Ltd., Yongin-si, Kyunggi-do 446-901 Korea) , Kim, Kyung-Ah (Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, 1, Hoegi, Dongdaemun-ku, Seoul, 130-701 Korea) , Ahn, Young-Tae (R &B D Center, Korea Yakult Co., Ltd., Yongin-si, Kyunggi-do 446-901 Korea) , Jeong, Jin-Ju (Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, 1, Hoegi, Dongdaemun-ku, Seoul, 130-701 Korea) , Huh, Chul-Sung (Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-742 Korea) , Kim, Dong-Hyun (Department of Life and Nanopharmaceutical Sciences, College of Pharmacy, 1, Hoegi, Dongdaemun-ku, Seoul, 130-701 Korea)

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BackgroundTo understand differences in the gut microbiota between elderly people of urbanized town communities (UTC) and longevity village communities (LVC), we analyzed fecal microbiota collected from individuals living in 2 UTC (Seoul and Chuncheon) and 3 LVC (Gurye, Damyang, and Soonchang) selected on the basis of indices for superlongevity (the ratio of centenarians to the total population) and longevity (the ratio of those aged 85 years or greater to those aged 65 years or greater) in South Korea by 454 pyrosequencing.ResultsTaxonomy-based analysis showed that The relative abundance of Firmicutes, Tenericutes, and Actinobacteria was significantly lower in LVC than in UTC. Due to an increase of Firmicutes and a reduction of Bacteroidetes, the ratio of Firmicutes to Bacteroidetes in the gut microbiota was greater in UTC adults than in UTC children or LVC adults. The population levels of Bacteroides, Prevotella, and Lachnospira were significantly higher in LVC than in UTC, but the levels of Dialister, Subdoligranulum, Megamonas, EF401882_g, and AM275436_g were lower in LVC than in UTC. Although most of the species detected in LVC were detected in UTC, some Bacteroides spp. and Faecalibacterium spp. were detected only in LVC. Among Bacteroides spp., ACWH_s, EF403317_s, and EF403722_s were detected in children and LVC samples only but FJ363527_s, 4P000677_s, and 4P000015_s were detected in UTC samples. EF402172_s and EF404388_s, members of Faecalibacterium spp., which are known to have anti-inflammatory properties, were detected in LVC and children only (>3.9% of total sequence). In addition, the fecal lipopolysaccharides (LPS) content was significantly higher in UTC than in LVC.ConclusionsThese findings suggest that maintaining gut microbiota, including Faecalibacterium spp. EF402172_s and EF404388_s, as well as low LPS levels may play an important role in preserving residents’ health in LVC.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-015-0386-8) contains supplementary material, which is available to authorized users.

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참고문헌 (32)

1. Alonso-Fernández P De la Fuente M Role of the immune system in aging and longevity Curr Aging Sci 2011 4 2 78 1002 10.2174/1874609811104020078 21235494
2. Human Microbiome Project Consortium Structure, function and diversity of the healthy human microbiome Nature 2012 486 7402 207 14 10.1038/nature11234 22699609

상세보기
3. Turnbaugh PJ Ley RE Mahowald MA Magrini V Mardis ER Gordon JI An obesity-associated gut microbiome with increased capacity for energy harvest Nature 2006 444 7122 1027 31 10.1038/nature05414 17183312

상세보기
4. Zhang C Zhang M Wang S Han R Cao Y Hua W Interactions between gut microbiota, host genetics and diet relevant to development of metabolic syndromes in mice ISME J 2010 4 2 232 41 10.1038/ismej.2009.112 19865183

상세보기
5. Dethlefsen L Huse S Sogin ML Relman DA The pervasive effects of an antibiotic on the human gut microbiota, as revealed by deep 16S rRNA sequencing PLoS Biol 2008 6 11 e280 10.1371/journal.pbio.0060280 19018661

상세보기
6. Turnbaugh PJ Hamady M Yatsunenko T Cantarel BL Duncan A Ley RE A core gut microbiome in obese and lean twins Nature 2009 457 7228 480 4 10.1038/nature07540 19043404

상세보기
7. Cani PD Delzenne NM Involvement of the gut microbiota in the development of low grade inflammation associated with obesity: focus on this neglected partner Acta Gastroenterol Belg 2010 73 2 267 9 20690567

상세보기
8. Cani PD Osto M Geurts L Everard A Involvement of gut microbiota in the development of low-grade inflammation and type 2 diabetes associated with obesity Gut Microbes 2012 3 4 279 88 10.4161/gmic.19625 22572877

상세보기
9. Kim KA Gu W Lee IA Joh EH Kim DH High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the TLR4 signaling pathway PLoS One 2012 7 10 e47713 10.1371/journal.pone.0047713 23091640

상세보기
10. Ottaviani E Ventura N Mandrioli M Candela M Franchini A Franceschi C Gut microbiota as a candidate for lifespan extension: an ecological/evolutionary perspective targeted on living organisms as metaorganisms Biogerontology 2011 12 6 599 609 10.1007/s10522-011-9352-5 21814818

상세보기
11. Biagi E Candela M Fairweather-Tait S Franceschi C Brigidi P Aging of the human metaorganism: the microbial counterpart Age (Dordr) 2012 34 1 247 67 10.1007/s11357-011-9217-5 21347607

상세보기
12. Tiihonen K Ouwehand AC Rautonen N Human intestinal microbiota and healthy ageing Ageing Res Rev 2009 9 2 107 16 10.1016/j.arr.2009.10.004 19874918

상세보기
13. Drago L Toscano M Rodighiero V De Vecchi E Mogna G Cultivable and pyrosequenced fecal microflora in centenarians and young subjects J Clin Gastroenterol 2012 46 Suppl S81 4 10.1097/MCG.0b013e3182693982 22955365

상세보기
14. Biagi E Candela M Turroni S Garagnani P Franceschi C Brigidi P Ageing and gut microbes: perspectives for health maintenance and longevity Pharmacol Res 2013 69 1 11 20 10.1016/j.phrs.2012.10.005 23079287

상세보기
15. KSI (Korea Statistical Information) Homepage. Availale at: http://www.nso.go.kr .
16. Park SC Park SC Korean centenarians-overall Korean Centenarians 2002 Seoul, Korea Seoul National University Press
17. Chun J Kim KY Lee JH Choi YN The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer BMC Microbiol 2010 10 101 10.1186/1471-2180-10-101 20370919

상세보기
18. Kim OS Cho YJ Lee K Yoon SH Kim M Na H Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species Int J Syst Evol Microbiol 2012 62 716 21 10.1099/ijs.0.038075-0 22140171

상세보기
19. Clark DA Chaouat G Banwatt D Friebe A Arck PC Ecology of danger-dependent cytokine-boosted spontaneous abortion in the CBA x DBA/2 mouse model: II. Fecal LPS levels in colonies with different basal abortion rates Am J Reprod Immunol 2008 60 529 33 10.1111/j.1600-0897.2008.00652.x 19032614

상세보기
20. Sokol H Pigneur B Watterlot L Lakhdari O Bermúdez-Humarán LG Gratadoux JJ Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients Proc Natl Acad Sci U S A 2008 105 43 16731 6 10.1073/pnas.0804812105 18936492

상세보기
21. Mitsuoka T Bifidobacteria and their role in human health J Ind Microbiol 1990 6 263 7 10.1007/BF01575871

상세보기
22. Hopkins MJ Sharp R Macfarlane GT Age and disease related changes in intestinal bacterial populations assessed by cell culture, 16S rRNA abundance, and community cellular fatty acid profiles Gut 2001 48 2 198 205 10.1136/gut.48.2.198 11156640

상세보기
23. Rajilić-Stojanović M Heilig HG Molenaar D Kajander K Surakka A Smidt H Development and application of the human intestinal tract chip, a phylogenetic microarray: analysis of universally conserved phylotypes in the abundant microbiota of young and elderly adults Environ Microbiol 2009 11 7 1736 51 10.1111/j.1462-2920.2009.01900.x 19508560

상세보기
24. Hayashi H Sakamoto M Kitahara M Benno Y Molecular analysis of fecal microbiota in elderly individuals using 16S rDNA library and T-RFLP Microbiol Immunol 2003 47 8 557 70 10.1111/j.1348-0421.2003.tb03418.x 14524616

상세보기
25. Biagi E Nylund L Candela M Rita O Laura B Elisa P Through ageing, and beyond: gut microbiota and inflammatory status in seniors and centenarians PLoS One 2010 5 5 e10667 10.1371/journal.pone.0010667 20498852

상세보기
26. Mueller S Saunier K Hanisch C Norin E Alm L Midtvedt T Differences in fecal microbiota in different European study populations in relation to age, gender, and country: a cross-sectional study Appl Environ Microbiol 2006 72 2 1027 33 10.1128/AEM.72.2.1027-1033.2006 16461645

상세보기
27. Mäkivuokko H Tiihonen K Tynkkynen S Paulin L Rautonen N The effect of age and non-steroidal anti-inflammatory drugs on human intestinal microbiota composition Br J Nutr 2010 103 227 34 10.1017/S0007114509991553 19703328

상세보기
28. Nam YD Jung MJ Roh SW Kim MS Bae JW Comparative analysis of Korean human gut microbiota by barcoded pyrosequencing PLoS One 2013 6 7 e22109 10.1371/journal.pone.0022109 21829445

상세보기
29. Kim KA Jung IH Park Park SH Ahn YT Huh CS Kim DH Comparative analysis of the gut microbiota in people with different levels of ginsenoside Rb1 degradation to compound K PLoS One 2013 8 4 e62409 10.1371/journal.pone.0062409 23638073

상세보기
30. Yon MY Lee MS Oh SI Park SC Kwak CS Assessment of Food Consumption, Dietary Diversity and Dietary Pattern during the Summer in Middle Aged Adults and Older Adults Living in Gugoksoondam Logevity Area Korea Korean J Community Nutr 2010 15 4 536 49
31. Kim Y Seo S Oran K Mi Sook C Comparisons of dietary behavior, food intake, and satisfaction with food-related life between the elderly living in urban and rural areas Korean J Nutr 2012 45 3 252 63 10.4163/kjn.2012.45.3.252

원문보기 상세보기
32. Chung HY Cesari M Anton S Marzetti E Giovannini S Seo AY Molecular Inflammation: Underpinnings of Aging and Age-related Diseases Ageing Res Rev 2009 8 1 18 30 10.1016/j.arr.2008.07.002 18692159

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Comparative analysis of gut microbiota in elderly people of urbanized towns and longevity villages 원문보기

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Comparative analysis of gut microbiota in elderly people of urbanized towns and longevity villages 원문보기

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