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Benfotiamine upregulates antioxidative system in activated BV-2 microglia cells 원문보기

Frontiers in cellular neuroscience, v.9, 2015년, pp.351 -   

Bozic, Iva (Institute for Biological Research “Siniš) ,  Savic, Danijela (a Stanković) ,  Stevanovic, Ivana (,” University of Belgrade Belgrade, Serbia) ,  Pekovic, Sanja (Institute for Biological Research “Siniš) ,  Nedeljkovic, Nadezda (a Stanković) ,  Lavrnja, Irena (,” University of Belgrade Belgrade, Serbia)

Abstract AI-Helper 아이콘AI-Helper

Chronic microglial activation and resulting sustained neuroinflammatory reaction are generally associated with neurodegeneration. Activated microglia acquires proinflammatory cellular profile that generates oxidative burst. Their persistent activation exacerbates inflammation, which damages healthy ...

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참고문헌 (79)

  1. Aguzzi A. Barres B. A. Bennett M. L. ( 2013 ). Microglia: scapegoat, saboteur, or something else? Science 339 , 156 – 161 . 10.1126/science.1227901 23307732 

  2. Andersen J. K. ( 2004 ). Oxidative stress in neurodegeneration: cause or consequence? Nat. Rev. Neurosci. 5 , S18 – S25 . 10.1038/nrn1434 

  3. Anderson M. E. ( 1986 ). Tissue glutathione , in Handbook of Methods for Oxygen Radical Research , ed Greenwald R. A. ( Boca Raton, FL : CRC Press ), 317 – 323 . 

  4. Andrades M. É. Morina A. Spasic S. Spasojevic I. ( 2011 ). Bench-to-bedside review: sepsis - from the redox point of view . Crit. Care 15 , 230 . 10.1186/cc10334 21996422 

  5. Auclair C. Voisin E. ( 1985 ). Nitroblue tetrazolium reduction , in Handbook of Methods for Oxygen Radical Research , ed Greenwald R. A. ( Boca Raton, FL : CRC Press ), 123 – 132 . 

  6. Balakumar P. Rohilla A. Krishan P. Solairaj P. Thangathirupathi A. ( 2010 ). The multifaceted therapeutic potential of benfotiamine . Pharmacol. Res. 61 , 482 – 488 . 10.1016/j.phrs.2010.02.008 20188835 

  7. Balakumar P. Sharma R. Singh M. ( 2008 ). Benfotiamine attenuates nicotine and uric acid-induced vascular endothelial dysfunction in the rat . Pharmacol. Res. 58 , 356 – 363 . 10.1016/j.phrs.2008.09.012 18951979 

  8. Barger S. W. Goodwin M. E. Porter M. M. Beggs M. L. ( 2007 ). Glutamate release from activated microglia requires the oxidative burst and lipid peroxidation . J. Neurochem. 101 , 1205 – 1213 . 10.1111/j.1471-4159.2007.04487.x 17403030 

  9. Barnham K. J. Masters C. L. Bush A. I. ( 2004 ). Neurodegenerative diseases and oxidative stress . Nat. Rev. Drug. Discov. 3 , 205 – 214 . 10.1038/nrd1330 15031734 

  10. Bitsch R. Wolf M. Möller J. Heuzeroth L. Grüneklee D. ( 1991 ). Bioavailability assessment of the lipophilic benfotiamine as compared to a water-soluble thiamin derivative . Ann. Nutr. Metab. 35 , 292 – 296 . 10.1159/000177659 1776825 

  11. Blasi E. Barluzzi R. Bocchini V. Mazzolla R. Bistoni F. ( 1990 ). Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus . J. Neuroimmunol. 27 , 229 – 237 . 10.1016/0165-5728(90)90073-V 2110186 

  12. Block M. L. Hong J. S. ( 2005 ). Microglia and inflammation-mediated neurodegeneration: multiple triggers with a common mechanism . Prog. Neurobiol. 76 , 77 – 98 . 10.1016/j.pneurobio.2005.06.004 16081203 

  13. Block M. L. Zecca L. Hong J. S. ( 2007 ). Microglia-mediated neurotoxicity: uncovering the molecular mechanisms . Nat. Rev. Neurosci. 8 , 57 – 69 . 10.1038/nrn2038 17180163 

  14. Bordt E. A. Polster B. M. ( 2014 ). NADPH oxidase- and mitochondria-derived reactive oxygen species in proinflammatory microglial activation: a bipartisan affair? Free Radic. Biol. Med. 76 , 34 – 46 . 10.1016/j.freeradbiomed.2014.07.033 25091898 

  15. Bozic I. Savic D. Laketa D. Bjelobaba I. Milenkovic I. Pekovic S. . ( 2015 ). Benfotiamine attenuates inflammatory response in LPS stimulated BV-2 microglia . PLoS ONE 10 : e0118372 . 10.1371/journal.pone.0118372 25695433 

  16. Brown G. C. ( 1995 ). Reversible binding and inhibition of catalase by nitric oxide . Eur. J. Biochem. 232 , 188 – 191 . 10.1111/j.1432-1033.1995.tb20798.x 7556149 

  17. Calingasan N. Y. Erdely H. A. Altar C. A. ( 2002 ). Identification of CD40 ligand in Alzheimer's disease and in animal models of Alzheimer's disease and brain injury . Neurobiol. Aging 23 , 31 – 39 . 10.1016/S0197-4580(01)00246-9 11755016 

  18. Ceylan-Isik A. F. Wu S. Li Q. Li S. Y. Ren J. ( 2006 ). High-dose benfotiamine rescues cardiomyocyte contractile dysfunction in streptozotocin-induced diabetes mellitus . J. Appl. Physiol. 1 , 150 – 156 . 10.1152/japplphysiol.00988.2005 16166234 

  19. Chen K. Huang J. Gong W. Zhang L. Yu P. Wang J. M. ( 2006 ). CD40/CD40L dyad in the inflammatory and immune responses in the central nervous system . Cell. Mol. Immunol. 3 , 163 – 169 . 16893496 

  20. Curtis C. Landis G. N. Folk D. Wehr N. B. Hoe N. Waskar M. . ( 2007 ). Transcriptional profiling of MnSOD-mediated lifespan extension in Drosophila reveals a species-general network of aging and metabolic genes . Genome Biol. 8 : R262 . 10.1186/gb-2007-8-12-r262 18067683 

  21. D'Aversa T. G. Weidenheim K. M. Berman J. W. ( 2002 ). CD40-CD40L interactions induce chemokine expression by human microglia: implications for human immunodeficiency virus encephalitis and multiple sclerosis . Am. J. Pathol. 160 , 559 – 567 . 10.1016/S0002-9440(10)64875-4 11839576 

  22. Djukic M. M. Jovanovic M. D. Ninkovic M. Stevanovic I. Ilic K. Curcic M. . ( 2012 ). Protective role of glutathione reductase in paraquat induced neurotoxicity . Chem. Biol. Interact. 199 , 74 – 86 . 10.1016/j.cbi.2012.05.008 22721943 

  23. Dringen R. ( 2005 ). Oxidative and antioxidative potential of brain microglial cells . Antioxid. Redox Signal. 7 , 1223 – 1233 . 10.1089/ars.2005.7.1223 16115027 

  24. Frakes A. E. Ferraiuolo L. Haidet-Phillips A. M. Schmelzer L. Braun L. Miranda C. J. . ( 2014 ). Microglia induce motor neuron death via the classical NF-κB pathway in amyotrophic lateral sclerosis . Neuron 81 , 1009 – 1023 . 10.1016/j.neuron.2014.01.013 24607225 

  25. Francescutti D. Baldwin J. Lee L. Mutus B. ( 1996 ). Peroxynitrite modification of glutathione reductase: modeling studies and kinetic evidence suggest the modification of tyrosines at the glutathione disulfide binding site . Protein Eng. 9 , 189 – 194 . 10.1093/protein/9.2.189 9005440 

  26. Freifelder D. ( 1976 ). Physical Biochemistry. New York, NY : W. H. Freeman and Company . 

  27. Fujiwara M. ( 1954 ). Allithiamine: a newly found derivative of vitamin B . J. Biochem. 2 , 273 – 285 . 

  28. Gao H. M. Hong J. S. Zhang W. Liu B. ( 2003 ). Synergistic dopaminergic neurotoxicity of the pesticide rotenone and inflammogen lipopolysaccharide: relevance to the etiology of Parkinson's disease . J. Neurosci. 23 , 1228 – 1236 . 12598611 

  29. Gems D. Partridge L. ( 2008 ). Stress-response hormesis and aging: “that which does not kill us makes us stronger.” Cell Metab. 7 , 200 – 203 . 10.1016/j.cmet.2008.01.001 18316025 

  30. Gerritse K. Laman J. D. Noelle R. J. Aruffo A. Ledbetter J. A. Boersma W. J. . ( 1996 ). CD40-CD40 ligand interactions in experimental allergic encephalomyelitis and multiple sclerosis . Proc. Natl. Acad. Sci. U.S.A. 93 , 2499 – 2504 . 10.1073/pnas.93.6.2499 8637903 

  31. Gertig U. Hanisch U. K. ( 2014 ). Microglial diversity by responses and responders . Front. Cell Neurosci. 8 : 101 . 10.3389/fncel.2014.00101 24744702 

  32. Giunta B. Rezai-Zadeh K. Tan J. ( 2010 ). Impact of the CD40-CD40L dyad in Alzheimer's disease . CNS Neurol. Disord. Drug Targets 9 , 149 – 155 . 10.2174/187152710791012099 20205645 

  33. Giunti D. Parodi B. Cordano C. Uccelli A. Kerlero de Rosbo N. ( 2014 ). Can we switch microglia's phenotype to foster neuroprotection? Focus on multiple sclerosis . Immunology 141 , 328 – 339 . 10.1111/imm.12177 24116890 

  34. González H. Elgueta D. Montoya A. Pacheco R. ( 2014 ). Neuroimmune regulation of microglial activity involved in neuroinflammation and neurodegenerative diseases . J. Neuroimmunol. 274 , 1 – 13 . 10.1016/j.jneuroim.2014.07.012 25091432 

  35. Góth L. ( 1991 ). A simple method for determination of serum catalase activity and revision of reference range . Clin. Chim. Acta 196 , 143 – 151 . 10.1016/0009-8981(91)90067-M 2029780 

  36. Hammes H. P. Du X. Edelstein D. Taguchi T. Matsumura T. Ju Q. . ( 2003 ). Benfotiamine blocks three major pathways of hyperglycemic damage and prevents experimental diabetic retinopathy . Nat. Med. 9 , 294 – 299 . 10.1038/nm834 12592403 

  37. Harisa G. I. ( 2013 ). Benfotiamine enhances antioxidant defenses and protects against cisplatin-induced DNA damage in nephrotoxic rats . J. Biochem. Mol. Toxicol. 27 , 398 – 405 . 10.1002/jbt.21501 23716490 

  38. Henn A. Lund S. Hedtjärn M. Schrattenholz A. Pörzgen P. Leist M. ( 2009 ). The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation . ALTEX 26 , 83 – 94 . 19565166 

  39. Jana M. Dasgupta S. Liu X. Pahan K. ( 2002 ). Regulation of tumor necrosis factor-alpha expression by CD40 ligation in BV-2 microglial cells . J. Neurochem. 80 , 197 – 206 . 10.1046/j.0022-3042.2001.00691.x 11796758 

  40. Jana M. Liu X. Koka S. Ghosh S. Petro T. M. Pahan K. ( 2001 ). Ligation of CD40 stimulates the induction of nitric-oxide synthase in microglial cells . J. Biol. Chem. 276 , 44527 – 44533 . 10.1074/jbc.M106771200 11551948 

  41. Jekabsone A. Mander P. K. Tickler A. Sharpe M. Brown G. C. ( 2006 ). Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study . J. Neuroinflamm. 3 : 24 . 10.1186/1742-2094-3-24 16959029 

  42. Kim W. K. Ganea D. Jonakait G. M. ( 2002 ). Inhibition of microglial CD40 expression by pituitary adenylate cyclase-activating polypeptide is mediated by interleukin-10 . J. Neuroimmunol. 126 , 16 – 24 . 10.1016/S0165-5728(02)00059-0 12020953 

  43. Kraft A. D. Harry G. J. ( 2011 ). Features of microglia and neuroinflammation relevant to environmental exposure and neurotoxicity. Int. J. Environ. Res. Public Health 8 , 2980 – 3018 . 10.3390/ijerph8072980 21845170 

  44. Kumar A. Chen S. H. Kadiiska M. B. Hong J. S. Zielonka J. Kalyanaraman B. . ( 2014 ). Inducible nitric oxide synthase is key to peroxynitrite-mediated, LPS-induced protein radical formation in murine microglial BV2 cells . Free Radic. Biol. Med. 73 , 51 – 59 . 10.1016/j.freeradbiomed.2014.04.014 24746617 

  45. Li J. O W. Li W. Jiang Z. G. Ghanbari H. A. ( 2013 ). Oxidative stress and neurodegenerative disorders . Int. J. Mol. Sci. 14 , 24438 – 24475 . 10.3390/ijms141224438 24351827 

  46. Lin S. X. Lisi L. Dello Russo C. Polak P. E. Sharp A. Weinberg G. ( 2011 ). The anti-inflammatory effects of dimethyl fumarate in astrocytes involve glutathione and haem oxygenase-1 . ASN Neuro 3 , 75 – 84 . 10.1042/AN20100033 21382015 

  47. Lowry O. H. Rosebrough N. J. Farr A. L. Randall R. J. ( 1951 ). Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 , 265 – 275 . 14907713 

  48. Lu S. C. ( 2009 ). Regulation of glutathione synthesis . Mol. Aspects Med. 30 , 42 – 59 . 10.1016/j.mam.2008.05.005 18601945 

  49. Luo X. G. Ding J. Q. Chen S. D. ( 2010 ). Microglia in the aging brain: relevance to neurodegeneration . Mol. Neurodegener. 5 : 12 . 10.1186/1750-1326-5-12 20334662 

  50. Mander P. K. Jekabsone A. Brown G. C. ( 2006 ). Microglia proliferation is regulated by hydrogen peroxide from NADPH oxidase . J. Immunol. 176 , 1046 – 1052 . 10.4049/jimmunol.176.2.1046 16393992 

  51. Manzardo A. M. He J. Poje A. Penick E. C. Campbell J. Butler M. G. ( 2013 ). Double-blind, randomized placebo-controlled clinical trial of benfotiamine for severe alcohol dependence . Drug Alcohol Depend. 133 , 562 – 570 . 10.1016/j.drugalcdep.2013.07.035 23992649 

  52. Maral J. Puget K. Michelson A. M. ( 1977 ). Comparative study of superoxide dismutase, catalase and glutathione peroxidase levels in erythrocytes of different animals . Biochem. Biophys. Res. Commun. 77 , 1525 – 1535 . 10.1016/S0006-291X(77)80151-4 901548 

  53. Marnett L. J. Rowlinson S. W. Goodwin D. C. Kalgutkar A. S. Lanzo C. A. ( 1999 ). Arachidonic acid oxygenation by COX-1 and COX-2. Mechanisms of catalysis and inhibition . J. Biol. Chem. 274 , 22903 – 22906 . 10.1074/jbc.274.33.22903 10438452 

  54. Miljkovic D. BlaŽevski J. Petkovic F. Djedovic N. Momèilovic M. Stanisavljevic S. . ( 2015 ). A comparative analysis of multiple sclerosis-relevant anti-inflammatory properties of ethyl pyruvate and dimethyl fumarate . J. Immunol. 194 , 2493 – 2503 . 10.4049/jimmunol.1402302 25681336 

  55. Min K. J. Pyo H. K. Yang M. S. Ji K. A. Jou I. Joe E. H. ( 2004 ). Gangliosides activate microglia via protein kinase C and NADPH oxidase . Glia 48 , 197 – 206 . 10.1002/glia.20069 15390122 

  56. Morgan M. J. Liu Z. G. ( 2011 ). Crosstalk of reactive oxygen species and NF-κB signaling . Cell Res. 21 , 103 – 115 . 10.1038/cr.2010.178 21187859 

  57. Moskaug J. Ø, Carlsen, H. Myhrstad M. C. Blomhoff R. ( 2005 ). Polyphenols and glutathione synthesis regulation . Am. J. Clin. Nutr. 81 , 277S - 283S . 15640491 

  58. Navarro-Gonzálvez J. A. García-Benayas C. Arenas J. ( 1998 ). Semiautomated measurement of nitrate in biological fluids . Clin. Chem. 44 , 679 – 681 . 9510886 

  59. Pan X. Gong N. Zhao J. Yu Z. Gu F. Chen J. . ( 2010 ). Powerful beneficial effects of benfotiamine on cognitive impairment and beta-amyloid deposition in amyloid precursor protein/presenilin-1 transgenic mice . Brain 133 , 1342 – 1351 . 10.1093/brain/awq069 20385653 

  60. Park J. Choi H. Min J. S. Park S. J. Kim J. H. Park H. J. . ( 2013 ). Mitochondrial dynamics modulate the expression of pro-inflammatory mediators in microglial cells . J. Neurochem. 127 , 221 – 232 . 10.1111/jnc.12361 23815397 

  61. Qin H. Wilson C. A. Lee S. J. Zhao X. Benveniste E. N. ( 2005 ). LPS induces CD40 gene expression through the activation of NF-kappaB and STAT-1alpha in macrophages and microglia . Blood 106 , 3114 – 3122 . 10.1182/blood-2005-02-0759 16020513 

  62. Rojo A. I. McBean G. Cindric M. Egea J. López M. G. Rada P. . ( 2014 ). Redox control of microglial function: molecular mechanisms and functional significance . Antioxid. Redox Signal. 21 , 1766 – 1801 . 10.1089/ars.2013.5745 24597893 

  63. Roth T. L. Nayak D. Atanasijevic T. Koretsky A. P. Latour L. L. McGavern D. B. ( 2014 ). Transcranial amelioration of inflammation and cell death after brain injury . Nature 505 , 223 – 228 . 10.1038/nature12808 24317693 

  64. Schmid U. Stopper H. Heidland A. Schupp N. ( 2008 ). Benfotiamine exhibits direct antioxidative capacity and prevents induction of DNA damage in vitro . Diabetes Metab. Res. Rev. 24 , 371 – 377 . 10.1002/dmrr.860 18384109 

  65. Schupp N. Dette E. M. Schmid U. Bahner U. Winkler M. Heidland A. . ( 2008 ). Benfotiamine reduces genomic damage in peripheral lymphocytes of hemodialysis patients. Naunyn Schmiedebergs Arch. Pharmacol. 378 , 283 – 291 . 10.1007/s00210-008-0310-y 18509620 

  66. Siomek A. ( 2012 ). NF-κB signaling pathway and free radical impact . Acta Biochim. Pol. 59 , 323 – 331 . 22855720 

  67. Solito E. Sastre M. ( 2012 ). Microglia function in Alzheimer's disease . Front. Pharmacol . 3 : 14 . 10.3389/fphar.2012.00014 22363284 

  68. Stirban A. Negrean M. Stratmann B. Gawlowski T. Horstmann T. Götting C. . ( 2006 ). Benfotiamine prevents macro- and microvascular endothelial dysfunction and oxidative stress following a meal rich in advanced glycation end products in individuals with type 2 diabetes . Diabetes Care 29 , 2064 – 2071 . 10.2337/dc06-0531 16936154 

  69. Sun M. Zigman S. ( 1978 ). An improved spectrophotometric assay for superoxide dismutase based on epinephrine autoxidation. Anal. Biochem. 90 , 81 – 89 . 10.1016/0003-2697(78)90010-6 727489 

  70. Talalay P. Dinkova-Kostova A. T. Holtzclaw W. D. ( 2003 ). Importance of phase 2 gene regulation in protection against electrophile and reactive oxygen toxicity and carcinogenesis . Adv. Enzyme Regul. 43 , 121 – 134 . 10.1016/S0065-2571(02)00038-9 12791387 

  71. Uttara B. Singh A. V. Zamboni P. Mahajan R. T. ( 2009 ). Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options . Curr. Neuropharmacol. 7 , 65 – 74 . 10.2174/157015909787602823 19721819 

  72. Valdovinos-Flores C. Gonsebatt M. E. ( 2012 ). The role of amino acid transporters in GSH synthesis in the blood-brain barrier and central nervous system. Neurochem. Int. 61 , 405 – 414 . 10.1016/j.neuint.2012.05.019 22634224 

  73. Van der Perren A. Macchi F. Toelen J. Carlon M. S. Maris M. de Loor H. . ( 2015 ). FK506 reduces neuroinflammation and dopaminergic neurodegeneration in an α-synuclein-based rat model for Parkinson's disease . Neurobiol. Aging 36 , 1559 – 1568 . 10.1016/j.neurobiolaging.2015.01.014 25660193 

  74. Verma S. Reddy K. Balakumar P. ( 2010 ). The defensive effect of benfotiamine in sodium arsenite-induced experimental vascular endothelial dysfunction . Biol. Trace Elem. Res. 137 , 96 – 109 . 10.1007/s12011-009-8567-7 19943121 

  75. Villacara A. Kumami K. Yamamoto T. Mrsulja B. B. Spatz M. ( 1989 ). Ischemic modification of cerebrocortical membranes: 5-hydroxytryptamine receptors, fluidity, and inducible in vitro lipid peroxidation . J. Neurochem. 53 , 595 – 601 . 10.1111/j.1471-4159.1989.tb07375.x 2746237 

  76. Wilms H. Sievers J. Rickert U. Rostami-Yazdi M. Mrowietz U. Lucius R. ( 2010 ). Dimethylfumarate inhibits microglial and astrocytic inflammation by suppressing the synthesis of nitric oxide, IL-1beta, TNF-alpha and IL-6 in an in-vitro model of brain inflammation . J. Neuroinflamm. 7 : 30 . 10.1186/1742-2094-7-30 20482831 

  77. Wu S. Ren J. ( 2006 ). Benfotiamine alleviates diabetes-induced cerebral oxidative damage independent of advanced glycation end-product, tissue factor and TNF-α . Neurosci. Lett . 394 , 158 – 162 . 10.1016/j.neulet.2005.10.022 16260089 

  78. Yang N. C. Ho W. M. Chen Y. H. Hu M. L. ( 2002 ). A convenient one-step extraction of cellular ATP using boiling water for the luciferin-luciferase assay of ATP . Anal. Biochem. 306 , 323 – 327 . 10.1006/abio.2002.5698 12123672 

  79. Zhang M. An C. Gao Y. Leak R. K. Chen J. Zhang F. ( 2013 ). Emerging roles of Nrf2 and phase II antioxidant enzymes in neuroprotection . Prog. Neurobiol. 100 , 30 – 47 . 10.1016/j.pneurobio.2012.09.003 23025925 

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