[논문]Antioxidant activity and mechanism of Rhizoma Cimicifugae

Li, Xican; Lin, Jing; Gao, Yaoxiang; Han, Weijuan; Chen, Dongfeng

doi:10.1186/1752-153X-6-140

Antioxidant activity and mechanism of Rhizoma Cimicifugae 원문보기

Chemistry central journal, v.6, 2012년, pp.140 - 140

Li, Xican (School of Chinese Herbal Medicine, Guangzhou Higher Education Mega Center, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou 510006, China) , Lin, Jing (School of Chinese Herbal Medicine, Guangzhou Higher Education Mega Center, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou 510006, China) , Gao, Yaoxiang (School of Chinese Herbal Medicine, Guangzhou Higher Education Mega Center, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou 510006, China) , Han, Weijuan (School of Chinese Herbal Medicine, Guangzhou Higher Education Mega Center, Guangzhou University of Chinese Medicine, Waihuang East Road No.232, Guangzhou 510006, China) , Chen, Dongfeng (School of Basic Medical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006, China)

Abstract ▼ AI-Helper

BackgroundAs a typical Chinese herbal medicine, rhizoma Cimicifugae (RC, 升麻 in Chinese) possesses various pharmacological effects involved in antioxidant activity. However, its antioxidant activity has not been reported so far. The aim of the present study was to systematically evaluate the antioxidant ability of RC in vitro, then discuss the mechanism.MethodsFirstly, five RC extracts (i.e. petroleum ether extract PERC, ethyl acetate extract EARC, absolute ethanol extract AERC, 95% ethanol extract 95ERC, and water extract WRC) were prepared and determined by various antioxidant methods, including anti-lipidperoxidation, protection against DNA damage, ·OH scavenging, ·O2- scavenging, DPPH· (1,1-diphenyl-2-picryl-hydrazl radical) scavenging, ABTS+· (2,2’-azino-bis (3-ethylbenzo- thiazoline-6-sulfonic acid radical ion) scavenging, Cu2+-chelating, and Fe3+ reducing assays. Subsequently, we measured the chemical contents of five RC extracts, including total phenolics, total saponins, total sugars, caffeic acid, ferulic acid and isoferulic acid. Finally, we quantitatively analyzed the correlations between antioxidant levels (1/IC50 values) and chemical contents.ResultsIn the study, the antioxidant levels and chemical contents (including total phenolics, total saponins, total sugars, caffeic acid, ferulic acid and isoferulic acid) of five RC extracts were determined by various methods. In all antioxidant assays, five RC extracts increased the antioxidant levels in a dose-dependent manner. However, their antioxidant levels (IC50 values) and chemical contents significantly differed from each other. Quantitative analysis of the correlation showed that total phenolic was of significant positive correlations (average R value was 0.56) with antioxidant levels; In contrast, total sugars and total saponins had no positive correlation with antioxidant (the average R values were −0.20 and −0.26, for total sugars and total saponins, respectively); Among total phenolics, three phenolic acids (caffeic acid, ferulic acid and isoferulic acid) also displayed positive correlations (the average R values were 0.51, 0.50, and 0.51, for caffeic acid, ferulic acid and isoferulic acid, respectively).ConclusionsAs an effective antioxidant, Rhizoma Cimicifugae can protect DNA and lipids against oxidative damage. Its antioxidant ability can be responsible for its various pharmacological effects and may be mainly attributed to the existence of total phenolics, among which caffeic acid, ferulic acid and isoferulic acid are regarded as main bioactive components. Rhizoma Cimicifugae exerts its antioxidant effect through metal-chelating, and radical-scavenging which is via donating hydrogen atom (H·) and donating electron (e).

주제어

참고문헌 (30)

Leichert LI Gehrke F Gudiseva HV Blackwell T Ilbert M Walker AK Strahler JR Andrews PC Jakob U Quantifying changes in the thiol redox proteome upon oxidative stress in vivo Proc Natl Acad Sci USA 2008 105 8197 8202 10.1073/pnas.0707723105 18287020

상세보기
Halliwell B Gutteridge JM Oxygen toxicity, oxygen radicals, transition metals and disease Biochem J 1984 219 1 14 6326753

상세보기
Bhattacharjee S Deterding LJ Chatterjee S Jiang J Ehrenshaft M Lardinois O Ramirez DC Tomer KB Mason RP Site-specific radical formation in DNA induced by Cu(II)-H2O2 oxidizing system, using ESR, immuno-spin trapping, LC-MS, and MS/MS Free Radic Biol Med 2011 50 1536 1545 10.1016/j.freeradbiomed.2011.02.034 21382477

상세보기
Tao HJ Variorum of Shennong’s Classic of Materia Medica 1995 Beijing: People’s Medical Publishing House 45
Chinese Pharmacopoeia Commission The Pharmacopoeia of Chinese People’s Republic 2005 Beijing China: Chemical Industry Publishing House 50
Ye L Hu Z Du G Zhang J Dong Q Fu F Tian J Antidepressant-like effects of the extract from Cimicifuga foetida L J Ethnopharmacol http://dx.doi.org/10.1016/j.jep.2012.10.013
Kim SJ Kim MS Inhibitory effects of Cimicifugae rhizoma extracts on histamine, bradykinin and COX-2 mediated inflammatory actions Phytother Res 2000 14 596 600 10.1002/1099-1573(200012)14:8 3.0.CO;2-V 11113994

상세보기
Nisslein T Freudenstein J Effects of an isopropanolic extract of Cimicifuga racemosa on urinary crosslinks and other parameters of bone quality in an ovariectomized rat model of osteoporosis J Bone Miner Metab 2003 21 370 376 10.1007/s00774-003-0431-9 14586793

상세보기
Ahn BS Yang M Jang H Lee HJ Moon C Kim JC Jung U Jo SK Jang JS Kim SH Evaluation of the antiosteoporotic potential of Cimicifuga heracleifolia in female mice Phytother Res 2012 26 663 668 10.1002/ptr.3624 21987388

상세보기
Kim M Protective effect of Cimicifuga heracleifolia ethanol extract and its constituents against gastric injury J Health Sci 2011 3 289 292
Zheng RL Huang ZY Huang ZY, Zheng RL The definition and characteristic of free radicals Free Radical Biology 2007 Beijing China: Chinese Higher Education Press 7
Dizdaroglu M Jaruga P Birinciolgu M Rodriguez H Free radical-induced damage to DNA: mechanism and measurement Free Radic Biol Med 2002 32 1102 1115 10.1016/S0891-5849(02)00826-2 12031895

상세보기
Keyer K Imlay JA Superoxide accelerates DNA damage by elevating free-iron levels Proc Natl Acad Sci USA 1996 93 13635 13640 10.1073/pnas.93.24.13635 8942986

상세보기
Han WJ Li XC Antioxidant activity of aloeswood tea in vitro Spatula DD 2012 2 43 50 10.5455/spatula.20120331054309
Torreggiani A Tamba M Trinchero A Bonora S Copper (II)-quercetin complexes in aqueous solutions: spectroscopic and kinetic properties J Mol Struct 2005 280 759 766
Bondet V Brand-Williams W Berset C Kinetics and mechanisms of antioxidant activity using the DPPH free radical method LWT- Food Sci Technol 1997 30 609 615 10.1006/fstl.1997.0240

상세보기
Brand-Williams W Cuvelier ME Berset C Use of free radical method to evaluate antioxidant activity LWT- Food Sci Technol 1995 28 25 30 10.1016/S0023-6438(95)80008-5

상세보기
Tsimogiannis DI Oreopoulou V The contribution of flavonoid C-ring on the DPPH free radical scavenging efficiency. A kinetic approach for the 3’, 4’-hydroxy substituted members Innov Food Sci Emerg 2006 7 140 146 10.1016/j.ifset.2005.09.001

상세보기
Saito S Kawabata J Effects of electron-withdrawing substituents on DPPH radical scavenging reactions of protocatechuic acid and its analogues in alcoholic solvents Tetrahedron 2005 61 8101 8108 10.1016/j.tet.2005.06.040

상세보기
Prior RL Cao G In vivo total antioxidant capacity: comparison of different analytical methods Free Radic Biol Med 1999 27 1173 1181 10.1016/S0891-5849(99)00203-8 10641708

상세보기
Liu BB Chen SH Chen SB The chemical composition and research progress of anti-tumor activity of Cimicifuga Cent South Phar 2012 10 53 58
Gülçin I Antioxidant activity of caffeic acid (3,4-dihydroxycinnamic acid) Toxicology 2006 217 213 220 10.1016/j.tox.2005.09.011 16243424

상세보기
Wang XZ Li XC Chen DF Evaluation of antioxidant activity of isoferulic acid in vitro Nat Prod Commun 2011 6 1285 1288 21941899
Li XC Wu XT Huang L Correlation between antioxidant activities and phenolic contents of Radix Angelica Sinensis (Danggui) Molecules 2009 14 5349 5361 10.3390/molecules14125349 20032898

상세보기
Wang XZ Li XC Li HR Reassessment of antioxidant activity of baicalein in vitro Asian J Pharm Biol Res 2011 1 186 194
Li XC Chen C Systematic evaluation on antioxidant of magnolol in vitro Int Res J Pure Appl Chem 2012 2 68 76
Li XC Improved pyrogallol autoxidation method: a reliable and cheap superoxide-scavenging assay suitable for all antioxidants J Agric Food Chem 2012 60 6418 6424 10.1021/jf204970r 22656066

상세보기
Li XC Wang XZ Chen DF Chen SZ Antioxidant activity and mechanism of protocatechuic acid in vitro Funct Food Health Dis 2011 1 232 244
Oyaizu M Studies on product of browning reaction prepared from glucoseamine Jpn J Nutr 1986 44 307 315 10.5264/eiyogakuzashi.44.307

상세보기
Li XC Chen DF Mai Y Wen B Wang XZ Concordance between antioxidant activities in vitro and chemical components of Radix Astragali (Huangqi) Nat Prod Res 2012 26 1050 1053 10.1080/14786419.2010.551771 21978321

상세보기

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Antioxidant activity and mechanism of Rhizoma Cimicifugae 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (30)

이 논문을 인용한 문헌

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트